An pH-responsive liposome for the targeted treatment of Helicobacter pylori

Objectives With the increase of Helicobacter pylori (H. pylori) drug resistance, it is increasingly difficult to cure H. pylori fundamentally. Frequent and excessive use of antibiotics can lead to disturbances in the intestinal flora and even inflammatory bowel disease, so new drugs are urgently nee...

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Main Authors: Huihua Dong, Aixing Guan, Shixian Yang, Luyao Wang, Lijuan Zhao, Hao Chen, Hongyu Wei, Peipei Luo, Yanqiang Huang
Format: Article
Language:English
Published: Elsevier 2025-10-01
Series:Materials Today Bio
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590006425007641
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author Huihua Dong
Aixing Guan
Shixian Yang
Luyao Wang
Lijuan Zhao
Hao Chen
Hongyu Wei
Peipei Luo
Yanqiang Huang
author_facet Huihua Dong
Aixing Guan
Shixian Yang
Luyao Wang
Lijuan Zhao
Hao Chen
Hongyu Wei
Peipei Luo
Yanqiang Huang
author_sort Huihua Dong
collection DOAJ
description Objectives With the increase of Helicobacter pylori (H. pylori) drug resistance, it is increasingly difficult to cure H. pylori fundamentally. Frequent and excessive use of antibiotics can lead to disturbances in the intestinal flora and even inflammatory bowel disease, so new drugs are urgently needed. Luteolin (LUT) has been found to have antimicrobial effects, but its water solubility is very low, and the antimicrobial effect is not ideal. To improve the water solubility of LUT and enhance its antibacterial activity, we encapsulated it with liposomes and coated it with chitosan quaternary ammonium salts to increase its targeting and acid responsiveness.Results Compared with LUT, H-L-NPs solubility is significantly improved, and the MIC is reduced by 1024 times, which is also effective against drug-resistant strains. The effect of in vivo treatment is significantly better than that of clinical triple therapy. In addition, H-L-NPs have the advantages of high safety and specificity, which can target the treatment of gastric H. pylori and maintain the diversity of intestinal microbiota, and H. pylori is not easy to develop drug resistance, making it a very promising anti-H. pylori lead.In conclusion, H-L-NPs greatly improved the antimicrobial activity of LUT in vivo and in vitro, and possessed the antimicrobial advantages of high targeting, acid responsiveness, specificity, and low toxicity. It is a kind of anti-H. pylori agent with a broad application prospect.
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spelling doaj-art-a10a2278ba5046f3b32edcdafcddbc802025-08-20T05:07:28ZengElsevierMaterials Today Bio2590-00642025-10-013410219410.1016/j.mtbio.2025.102194An pH-responsive liposome for the targeted treatment of Helicobacter pyloriHuihua Dong0Aixing Guan1Shixian Yang2Luyao Wang3Lijuan Zhao4Hao Chen5Hongyu Wei6Peipei Luo7Yanqiang Huang8Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise, 533000, ChinaAffiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China; Guangxi Clinical Medical Research Center for Hepatobiliary Diseases, Baise, 533000, ChinaAffiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China; Guangxi Clinical Medical Research Center for Hepatobiliary Diseases, Baise, 533000, ChinaGuangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise, 533000, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Clinical Prevention and Control Technology and Leading Drug for Microorganisms with Drug Resistance in Border Ethnic Areasn, Baise, 533000, ChinaGuangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise, 533000, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Clinical Prevention and Control Technology and Leading Drug for Microorganisms with Drug Resistance in Border Ethnic Areasn, Baise, 533000, ChinaDepartment of Pathology, Wannan Medical College, Wuhu, 241002, ChinaAffiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China; Guangxi Clinical Medical Research Center for Hepatobiliary Diseases, Baise, 533000, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Clinical Prevention and Control Technology and Leading Drug for Microorganisms with Drug Resistance in Border Ethnic Areasn, Baise, 533000, China; Corresponding author. Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China.Department of Gastroenterology, Wujin People's Hospital Affiliated to JiangSu University, Changzhou, 213004, China; Corresponding author.Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China; Guangxi Clinical Medical Research Center for Hepatobiliary Diseases, Baise, 533000, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Clinical Prevention and Control Technology and Leading Drug for Microorganisms with Drug Resistance in Border Ethnic Areasn, Baise, 533000, China; Corresponding author. Guangxi Clinical Medical Research Center for Hepatobiliary Diseases, Baise, 533000, China.Objectives With the increase of Helicobacter pylori (H. pylori) drug resistance, it is increasingly difficult to cure H. pylori fundamentally. Frequent and excessive use of antibiotics can lead to disturbances in the intestinal flora and even inflammatory bowel disease, so new drugs are urgently needed. Luteolin (LUT) has been found to have antimicrobial effects, but its water solubility is very low, and the antimicrobial effect is not ideal. To improve the water solubility of LUT and enhance its antibacterial activity, we encapsulated it with liposomes and coated it with chitosan quaternary ammonium salts to increase its targeting and acid responsiveness.Results Compared with LUT, H-L-NPs solubility is significantly improved, and the MIC is reduced by 1024 times, which is also effective against drug-resistant strains. The effect of in vivo treatment is significantly better than that of clinical triple therapy. In addition, H-L-NPs have the advantages of high safety and specificity, which can target the treatment of gastric H. pylori and maintain the diversity of intestinal microbiota, and H. pylori is not easy to develop drug resistance, making it a very promising anti-H. pylori lead.In conclusion, H-L-NPs greatly improved the antimicrobial activity of LUT in vivo and in vitro, and possessed the antimicrobial advantages of high targeting, acid responsiveness, specificity, and low toxicity. It is a kind of anti-H. pylori agent with a broad application prospect.http://www.sciencedirect.com/science/article/pii/S2590006425007641LuteolinLiposomesChitosan quaternary ammonium saltAnti-H. pyloriAcid response
spellingShingle Huihua Dong
Aixing Guan
Shixian Yang
Luyao Wang
Lijuan Zhao
Hao Chen
Hongyu Wei
Peipei Luo
Yanqiang Huang
An pH-responsive liposome for the targeted treatment of Helicobacter pylori
Materials Today Bio
Luteolin
Liposomes
Chitosan quaternary ammonium salt
Anti-H. pylori
Acid response
title An pH-responsive liposome for the targeted treatment of Helicobacter pylori
title_full An pH-responsive liposome for the targeted treatment of Helicobacter pylori
title_fullStr An pH-responsive liposome for the targeted treatment of Helicobacter pylori
title_full_unstemmed An pH-responsive liposome for the targeted treatment of Helicobacter pylori
title_short An pH-responsive liposome for the targeted treatment of Helicobacter pylori
title_sort ph responsive liposome for the targeted treatment of helicobacter pylori
topic Luteolin
Liposomes
Chitosan quaternary ammonium salt
Anti-H. pylori
Acid response
url http://www.sciencedirect.com/science/article/pii/S2590006425007641
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