Bisphenol A degradation by halotolerant Bacillus HQ-51-Ba from deep sea environment and its metabolic pathway
Bisphenol A (BPA), as an endocrine disruptor, has estrogenic activity and disrupts the endocrine systems of humans and animals, leading to various health problems. Bacterial degradation is considered to be a cost effective and environmentally friendly method for BPA degradation compared to other tre...
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KeAi Communications Co., Ltd.
2025-01-01
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| Series: | Environmental Chemistry and Ecotoxicology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590182625000906 |
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| author | Xinyu Li Ruixia Yang Fengmei Wang Wucai Zhao Aifeng Liu Minggang Zheng Ling Wang Yinghua Lou |
| author_facet | Xinyu Li Ruixia Yang Fengmei Wang Wucai Zhao Aifeng Liu Minggang Zheng Ling Wang Yinghua Lou |
| author_sort | Xinyu Li |
| collection | DOAJ |
| description | Bisphenol A (BPA), as an endocrine disruptor, has estrogenic activity and disrupts the endocrine systems of humans and animals, leading to various health problems. Bacterial degradation is considered to be a cost effective and environmentally friendly method for BPA degradation compared to other treatment methods. HQ-51-Ba was isolated from sediment of the deep ocean hydrothermal region in the Atlantic Ocean, and showed high degradation capacity for BPA under salinity environment. By analyzing the morphology and 16S rRNA sequence results, HQ-51-Ba was identified as Bacillus sp. Additionally, the gene of spore coat protein A (CotA) was found in complete genome sequence, which showed laccase activity for BPA degradation. Compared to other BPA-degrading bacteria, HQ-51-Ba exhibits superior viability and reproductive capacity at 7 % salinity. Under optimal conditions, it achieved complete degradation of BPA (10 mg/L) within 11 h. The functional enzyme responsible for BPA degradation was validated as intracellular. Identification of five key intermediates enabled the proposal of a BPA degradation pathway for HQ-51-Ba. Notably, intermediates 4-hydroxybenzoic acid (HBA) and 4-hydroxyacetophenone (HAP) demonstrate lower toxicity than BPA. The results of this study not only screened a salt tolerant strain of BPA degrading bacteria, but also provided a promising approach for the treatment of BPA pollution in saline wastewater. |
| format | Article |
| id | doaj-art-a105c3e2c08e4f548fd899e5a8be72bc |
| institution | DOAJ |
| issn | 2590-1826 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Environmental Chemistry and Ecotoxicology |
| spelling | doaj-art-a105c3e2c08e4f548fd899e5a8be72bc2025-08-20T03:15:54ZengKeAi Communications Co., Ltd.Environmental Chemistry and Ecotoxicology2590-18262025-01-0171362136910.1016/j.enceco.2025.06.016Bisphenol A degradation by halotolerant Bacillus HQ-51-Ba from deep sea environment and its metabolic pathwayXinyu Li0Ruixia Yang1Fengmei Wang2Wucai Zhao3Aifeng Liu4Minggang Zheng5Ling Wang6Yinghua Lou7School of Environment and Geography, Qingdao University, Qingdao 266071, ChinaSchool of Environment and Geography, Qingdao University, Qingdao 266071, ChinaTechnology Center of Qingdao Customs, Qingdao 266109, ChinaSchool of Environment and Geography, Qingdao University, Qingdao 266071, ChinaSchool of Environment and Geography, Qingdao University, Qingdao 266071, China; Corresponding author.Marine Bioresources and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, 6 XianXiaLing Road, Qingdao, Shandong, ChinaSchool of Environment and Geography, Qingdao University, Qingdao 266071, ChinaSchool of Environment and Geography, Qingdao University, Qingdao 266071, ChinaBisphenol A (BPA), as an endocrine disruptor, has estrogenic activity and disrupts the endocrine systems of humans and animals, leading to various health problems. Bacterial degradation is considered to be a cost effective and environmentally friendly method for BPA degradation compared to other treatment methods. HQ-51-Ba was isolated from sediment of the deep ocean hydrothermal region in the Atlantic Ocean, and showed high degradation capacity for BPA under salinity environment. By analyzing the morphology and 16S rRNA sequence results, HQ-51-Ba was identified as Bacillus sp. Additionally, the gene of spore coat protein A (CotA) was found in complete genome sequence, which showed laccase activity for BPA degradation. Compared to other BPA-degrading bacteria, HQ-51-Ba exhibits superior viability and reproductive capacity at 7 % salinity. Under optimal conditions, it achieved complete degradation of BPA (10 mg/L) within 11 h. The functional enzyme responsible for BPA degradation was validated as intracellular. Identification of five key intermediates enabled the proposal of a BPA degradation pathway for HQ-51-Ba. Notably, intermediates 4-hydroxybenzoic acid (HBA) and 4-hydroxyacetophenone (HAP) demonstrate lower toxicity than BPA. The results of this study not only screened a salt tolerant strain of BPA degrading bacteria, but also provided a promising approach for the treatment of BPA pollution in saline wastewater.http://www.sciencedirect.com/science/article/pii/S2590182625000906Salt-tolerant bacteriaBisphenol ABiodegradationBacillus |
| spellingShingle | Xinyu Li Ruixia Yang Fengmei Wang Wucai Zhao Aifeng Liu Minggang Zheng Ling Wang Yinghua Lou Bisphenol A degradation by halotolerant Bacillus HQ-51-Ba from deep sea environment and its metabolic pathway Environmental Chemistry and Ecotoxicology Salt-tolerant bacteria Bisphenol A Biodegradation Bacillus |
| title | Bisphenol A degradation by halotolerant Bacillus HQ-51-Ba from deep sea environment and its metabolic pathway |
| title_full | Bisphenol A degradation by halotolerant Bacillus HQ-51-Ba from deep sea environment and its metabolic pathway |
| title_fullStr | Bisphenol A degradation by halotolerant Bacillus HQ-51-Ba from deep sea environment and its metabolic pathway |
| title_full_unstemmed | Bisphenol A degradation by halotolerant Bacillus HQ-51-Ba from deep sea environment and its metabolic pathway |
| title_short | Bisphenol A degradation by halotolerant Bacillus HQ-51-Ba from deep sea environment and its metabolic pathway |
| title_sort | bisphenol a degradation by halotolerant bacillus hq 51 ba from deep sea environment and its metabolic pathway |
| topic | Salt-tolerant bacteria Bisphenol A Biodegradation Bacillus |
| url | http://www.sciencedirect.com/science/article/pii/S2590182625000906 |
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