STUDY OF DENDRITIC CELL MIGRATION USING CELL-IQ ANALYSIS SYSTEM
Dendritic cells (DCs) belong to specialized pool of antigen-presenting cells with high functional plasticity and manifest with immunostimulatory or immunosuppressive potential depending on sequence and combination of microenvironment stimuli, which determine their differentiation, maturation and a...
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2018-09-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/810 |
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| author | T. L. Nekhaeva A. B. Danilova I. A. Baldueva |
| author_facet | T. L. Nekhaeva A. B. Danilova I. A. Baldueva |
| author_sort | T. L. Nekhaeva |
| collection | DOAJ |
| description | Dendritic cells (DCs) belong to specialized pool of antigen-presenting cells with high functional plasticity and manifest with immunostimulatory or immunosuppressive potential depending on sequence and combination of microenvironment stimuli, which determine their differentiation, maturation and activation. The use of antitumor DCs vaccines is based on the ability of DCs specifically activated in vitro migrate for antigen presentation to T- lymphocytes. We studied the components of the tumor microenvironment that are capable of inhibiting DCs migration. The study of the mobility of DCs in Cell-IQ experimental analytical system showed the presence of an inverse correlation of high strength between the average trajectory speed and the level of immunosuppressive factors (ISFs) in supernatants of cultured skin melanoma cells (TGFβ1, IL-10, IL-18, VEGF-A, EGF, FGF, HGF, sFASL (p<0.01). An inverse relation of high force was found between the movement angle of the DCs population and the expression of cancer testis antigens (CTAs) and other tumor- associated antigens (TAAs) on tumor cells (Melan A, tyrosinase, families of MAGE, BAGE, NY- ESO-1 (p<0.01)). The speed of DCs movement in culture system with melanoma cells #894 was 30.10±2.23 μm/h and differed from that in the presence of IL-10 1 ng/ml (10.45±0.52 μm/h), TGFβ1 10 ng/ml (14.32±0.42 μm/h), VEGF 50 ng/ml (18.7±1.89 μm/h) (p<0.05). One can assume that content of this ISFs in the blood is one of the factors determining clinical efficacy of DCs immune therapy. |
| format | Article |
| id | doaj-art-a1044c73682a4bd386da4ae8c409dc6b |
| institution | DOAJ |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2018-09-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-a1044c73682a4bd386da4ae8c409dc6b2025-08-20T03:18:26ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682018-09-01174142310.21294/1814-4861-2018-17-4-14-23556STUDY OF DENDRITIC CELL MIGRATION USING CELL-IQ ANALYSIS SYSTEMT. L. Nekhaeva0A. B. Danilova1I. A. Baldueva2National Medical Research Centre of Oncology named after N.N. Petrov, Ministry of Health of RussiaNational Medical Research Centre of Oncology named after N.N. Petrov, Ministry of Health of RussiaNational Medical Research Centre of Oncology named after N.N. Petrov, Ministry of Health of RussiaDendritic cells (DCs) belong to specialized pool of antigen-presenting cells with high functional plasticity and manifest with immunostimulatory or immunosuppressive potential depending on sequence and combination of microenvironment stimuli, which determine their differentiation, maturation and activation. The use of antitumor DCs vaccines is based on the ability of DCs specifically activated in vitro migrate for antigen presentation to T- lymphocytes. We studied the components of the tumor microenvironment that are capable of inhibiting DCs migration. The study of the mobility of DCs in Cell-IQ experimental analytical system showed the presence of an inverse correlation of high strength between the average trajectory speed and the level of immunosuppressive factors (ISFs) in supernatants of cultured skin melanoma cells (TGFβ1, IL-10, IL-18, VEGF-A, EGF, FGF, HGF, sFASL (p<0.01). An inverse relation of high force was found between the movement angle of the DCs population and the expression of cancer testis antigens (CTAs) and other tumor- associated antigens (TAAs) on tumor cells (Melan A, tyrosinase, families of MAGE, BAGE, NY- ESO-1 (p<0.01)). The speed of DCs movement in culture system with melanoma cells #894 was 30.10±2.23 μm/h and differed from that in the presence of IL-10 1 ng/ml (10.45±0.52 μm/h), TGFβ1 10 ng/ml (14.32±0.42 μm/h), VEGF 50 ng/ml (18.7±1.89 μm/h) (p<0.05). One can assume that content of this ISFs in the blood is one of the factors determining clinical efficacy of DCs immune therapy.https://www.siboncoj.ru/jour/article/view/810dendritic cellsmigrationimmunosuppressive factorscancer testis antigenscell-iq. |
| spellingShingle | T. L. Nekhaeva A. B. Danilova I. A. Baldueva STUDY OF DENDRITIC CELL MIGRATION USING CELL-IQ ANALYSIS SYSTEM Сибирский онкологический журнал dendritic cells migration immunosuppressive factors cancer testis antigens cell-iq. |
| title | STUDY OF DENDRITIC CELL MIGRATION USING CELL-IQ ANALYSIS SYSTEM |
| title_full | STUDY OF DENDRITIC CELL MIGRATION USING CELL-IQ ANALYSIS SYSTEM |
| title_fullStr | STUDY OF DENDRITIC CELL MIGRATION USING CELL-IQ ANALYSIS SYSTEM |
| title_full_unstemmed | STUDY OF DENDRITIC CELL MIGRATION USING CELL-IQ ANALYSIS SYSTEM |
| title_short | STUDY OF DENDRITIC CELL MIGRATION USING CELL-IQ ANALYSIS SYSTEM |
| title_sort | study of dendritic cell migration using cell iq analysis system |
| topic | dendritic cells migration immunosuppressive factors cancer testis antigens cell-iq. |
| url | https://www.siboncoj.ru/jour/article/view/810 |
| work_keys_str_mv | AT tlnekhaeva studyofdendriticcellmigrationusingcelliqanalysissystem AT abdanilova studyofdendriticcellmigrationusingcelliqanalysissystem AT iabaldueva studyofdendriticcellmigrationusingcelliqanalysissystem |