Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome
Acute coronary syndrome (ACS) can be triggered by the presence of inflammatory factors which promote the activation of immune cells by costimulatory molecules such as CD40 and its ligand CD40L. Environmental and genetic factors are involved in the etiology of the ACS. The aim of this study was to ex...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/8063983 |
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| author | D. E. Martínez-Fernández J. R. Padilla-Gutiérrez F. Casillas-Muñoz Emmanuel Valdés-Alvarado Brenda Parra-Reyna Maricela Aceves-Ramírez J. F. Muñoz-Valle U. Zalapa Flores J. C. Chávez Herrera Y. Valle |
| author_facet | D. E. Martínez-Fernández J. R. Padilla-Gutiérrez F. Casillas-Muñoz Emmanuel Valdés-Alvarado Brenda Parra-Reyna Maricela Aceves-Ramírez J. F. Muñoz-Valle U. Zalapa Flores J. C. Chávez Herrera Y. Valle |
| author_sort | D. E. Martínez-Fernández |
| collection | DOAJ |
| description | Acute coronary syndrome (ACS) can be triggered by the presence of inflammatory factors which promote the activation of immune cells by costimulatory molecules such as CD40 and its ligand CD40L. Environmental and genetic factors are involved in the etiology of the ACS. The aim of this study was to explore the gene and protein expression associated with CD40 and CD40L genetic variants in ACS patients from the western Mexican population. A total of 620 individuals from western Mexico were recruited: 320 ACS patients and 300 individuals without a history of ischemic cardiopathy were evaluated. The genotype was determined using TaqMan SNP genotyping assays. CD40 and CD40L expressions at the mRNA level were quantified using TaqMan Gene Expression Assays. Soluble protein isoforms were measured by enzyme-linked immunosorbent assay. We did not find evidence of association between CD40 (rs1883832, rs4810485, and rs11086998) and CD40L (rs3092952 and rs3092920) genetic variants and susceptibility to ACS, although rs1883832 and rs4810485 were significantly associated with high sCD40 plasma levels. Plasma levels of sCD40L can be affected by gender and the clinical spectrum of acute coronary syndrome. Our results do not suggest a functional role of CD40 and CD40L genetic variants in ACS. However, they could reflect the inflammatory process and platelet activation in ACS patients, even when they are under pharmacological therapy. Due to the important roles of the CD40-CD40L system in the pathogenesis of ACS, longitudinal studies are required to determine if soluble levels of CD40 and CD40L could be clinically useful markers of a recurrent cardiovascular event after an ACS. |
| format | Article |
| id | doaj-art-a1028553e78646ed88154b0086834313 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-a1028553e78646ed88154b00868343132025-02-03T01:32:04ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/80639838063983Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary SyndromeD. E. Martínez-Fernández0J. R. Padilla-Gutiérrez1F. Casillas-Muñoz2Emmanuel Valdés-Alvarado3Brenda Parra-Reyna4Maricela Aceves-Ramírez5J. F. Muñoz-Valle6U. Zalapa Flores7J. C. Chávez Herrera8Y. Valle9Instituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Sierra Mojada #950, Colonia Independencia, C.P, 44340 Guadalajara, Jalisco, MexicoInstituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Sierra Mojada #950, Colonia Independencia, C.P, 44340 Guadalajara, Jalisco, MexicoInstituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Sierra Mojada #950, Colonia Independencia, C.P, 44340 Guadalajara, Jalisco, MexicoInstituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Sierra Mojada #950, Colonia Independencia, C.P, 44340 Guadalajara, Jalisco, MexicoInstituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Sierra Mojada #950, Colonia Independencia, C.P, 44340 Guadalajara, Jalisco, MexicoInstituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Sierra Mojada #950, Colonia Independencia, C.P, 44340 Guadalajara, Jalisco, MexicoInstituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Sierra Mojada #950, Colonia Independencia, C.P, 44340 Guadalajara, Jalisco, MexicoEspecialidad en Cardiología, Unidad Médica de Alta Especialidad, Centro Médico Nacional de Occidente (CMNO), Departamento de Cardiología, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MexicoEspecialidad en Cardiología, Unidad Médica de Alta Especialidad, Centro Médico Nacional de Occidente (CMNO), Departamento de Cardiología, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MexicoInstituto de Investigación en Ciencias Biomédicas (IICB), Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Sierra Mojada #950, Colonia Independencia, C.P, 44340 Guadalajara, Jalisco, MexicoAcute coronary syndrome (ACS) can be triggered by the presence of inflammatory factors which promote the activation of immune cells by costimulatory molecules such as CD40 and its ligand CD40L. Environmental and genetic factors are involved in the etiology of the ACS. The aim of this study was to explore the gene and protein expression associated with CD40 and CD40L genetic variants in ACS patients from the western Mexican population. A total of 620 individuals from western Mexico were recruited: 320 ACS patients and 300 individuals without a history of ischemic cardiopathy were evaluated. The genotype was determined using TaqMan SNP genotyping assays. CD40 and CD40L expressions at the mRNA level were quantified using TaqMan Gene Expression Assays. Soluble protein isoforms were measured by enzyme-linked immunosorbent assay. We did not find evidence of association between CD40 (rs1883832, rs4810485, and rs11086998) and CD40L (rs3092952 and rs3092920) genetic variants and susceptibility to ACS, although rs1883832 and rs4810485 were significantly associated with high sCD40 plasma levels. Plasma levels of sCD40L can be affected by gender and the clinical spectrum of acute coronary syndrome. Our results do not suggest a functional role of CD40 and CD40L genetic variants in ACS. However, they could reflect the inflammatory process and platelet activation in ACS patients, even when they are under pharmacological therapy. Due to the important roles of the CD40-CD40L system in the pathogenesis of ACS, longitudinal studies are required to determine if soluble levels of CD40 and CD40L could be clinically useful markers of a recurrent cardiovascular event after an ACS.http://dx.doi.org/10.1155/2019/8063983 |
| spellingShingle | D. E. Martínez-Fernández J. R. Padilla-Gutiérrez F. Casillas-Muñoz Emmanuel Valdés-Alvarado Brenda Parra-Reyna Maricela Aceves-Ramírez J. F. Muñoz-Valle U. Zalapa Flores J. C. Chávez Herrera Y. Valle Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome Journal of Immunology Research |
| title | Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome |
| title_full | Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome |
| title_fullStr | Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome |
| title_full_unstemmed | Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome |
| title_short | Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome |
| title_sort | analysis of genetic variation in cd40 and cd40l relationship with mrna relative expression and soluble proteins in acute coronary syndrome |
| url | http://dx.doi.org/10.1155/2019/8063983 |
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