Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences
Puccinia triticina, the causal agent of wheat leaf rust, is one of the most devastating rust fungi attacking wheat worldwide. Seventy-six isolates of the wheat leaf rust pathogen from Yunnan, Sichuan, Gansu and Henan provinces, China, were tested on wheat leaf rust differentials and the population s...
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KeAi Communications Co., Ltd.
2018-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311918619239 |
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| author | Tai-guo LIU Run-jing GE Yu-tong MA Bo LIU Li GAO Wan-quan CHEN |
| author_facet | Tai-guo LIU Run-jing GE Yu-tong MA Bo LIU Li GAO Wan-quan CHEN |
| author_sort | Tai-guo LIU |
| collection | DOAJ |
| description | Puccinia triticina, the causal agent of wheat leaf rust, is one of the most devastating rust fungi attacking wheat worldwide. Seventy-six isolates of the wheat leaf rust pathogen from Yunnan, Sichuan, Gansu and Henan provinces, China, were tested on wheat leaf rust differentials and the population structure was analyzed using four presumably neutral partial sequence markers such as elongation factor-1α (EF-1α), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin (TUB) and the second largest RNA polymerase subunit (RPB2). The phenotypic diversity of Yunnan and Sichuan populations was higher than that of Gansu and Henan populations. The four populations were separated into two clusters based on the pathogenic data. A total of 12 single nucleotide polymorphisms (SNPs) and 32 haplotypes were identified among the four sequences. The 32 haplotypes were divided into two clusters in a neighbor-joining tree. Bayesian analyses also identified two clusters. Pairwise FST between populations in different regions were significantly different (P<0.05). Analysis of molecular variance (AMOVA) indicated that 68% of the total genetic variation was within populations. |
| format | Article |
| id | doaj-art-a0ff43dd86e441a788a19cc764e3a52e |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-a0ff43dd86e441a788a19cc764e3a52e2025-08-20T03:56:59ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-08-011781779178910.1016/S2095-3119(18)61923-9Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequencesTai-guo LIU0Run-jing GE1Yu-tong MA2Bo LIU3Li GAO4Wan-quan CHEN5Correspondence LIU Tai-guo, Tel: +86-10-62815618, Fax: +86-10-62815909; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaPuccinia triticina, the causal agent of wheat leaf rust, is one of the most devastating rust fungi attacking wheat worldwide. Seventy-six isolates of the wheat leaf rust pathogen from Yunnan, Sichuan, Gansu and Henan provinces, China, were tested on wheat leaf rust differentials and the population structure was analyzed using four presumably neutral partial sequence markers such as elongation factor-1α (EF-1α), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin (TUB) and the second largest RNA polymerase subunit (RPB2). The phenotypic diversity of Yunnan and Sichuan populations was higher than that of Gansu and Henan populations. The four populations were separated into two clusters based on the pathogenic data. A total of 12 single nucleotide polymorphisms (SNPs) and 32 haplotypes were identified among the four sequences. The 32 haplotypes were divided into two clusters in a neighbor-joining tree. Bayesian analyses also identified two clusters. Pairwise FST between populations in different regions were significantly different (P<0.05). Analysis of molecular variance (AMOVA) indicated that 68% of the total genetic variation was within populations.http://www.sciencedirect.com/science/article/pii/S2095311918619239population structurepolymorphismvirulencewheat leaf rustPuccinia triticina |
| spellingShingle | Tai-guo LIU Run-jing GE Yu-tong MA Bo LIU Li GAO Wan-quan CHEN Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences Journal of Integrative Agriculture population structure polymorphism virulence wheat leaf rust Puccinia triticina |
| title | Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences |
| title_full | Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences |
| title_fullStr | Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences |
| title_full_unstemmed | Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences |
| title_short | Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences |
| title_sort | population genetic structure of chinese puccinia triticina races based on multi locus sequences |
| topic | population structure polymorphism virulence wheat leaf rust Puccinia triticina |
| url | http://www.sciencedirect.com/science/article/pii/S2095311918619239 |
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