Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines
Leaf width is a key determinant of planting density and photosynthetic efficiency. In an effort to determine which genes regulate maize plant leaf width, we performed a genome-wide association study (GWAS) of 1.49 × 106 single nucleotide polymorphisms (SNPs) in 80 sequenced backbone inbred maize lin...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-08-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/14/9/1057 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850260557057228800 |
|---|---|
| author | Shi Lu Qi Wang Junqi Yin Shubo Zheng Tingting Gao Xudong Zhou Jianxin Zhang Yuexian Xing Yingjie Ma Min Wang Delong Zhou Ming Lu Wenguo Liu Piwu Wang Zhijun Zhang |
| author_facet | Shi Lu Qi Wang Junqi Yin Shubo Zheng Tingting Gao Xudong Zhou Jianxin Zhang Yuexian Xing Yingjie Ma Min Wang Delong Zhou Ming Lu Wenguo Liu Piwu Wang Zhijun Zhang |
| author_sort | Shi Lu |
| collection | DOAJ |
| description | Leaf width is a key determinant of planting density and photosynthetic efficiency. In an effort to determine which genes regulate maize plant leaf width, we performed a genome-wide association study (GWAS) of 1.49 × 106 single nucleotide polymorphisms (SNPs) in 80 sequenced backbone inbred maize lines in Jilin Province, China, based upon phenotypic leaf width data from two years. In total, 14 SNPs were identified as being significantly related to leaf width (<i>p</i> < 0.000001), with these SNPs being located on chromosomes 1, 2, 3, 5, 6, 7, 8, and 9. A total of five candidate genes were identified within a mean linkage disequilibrium (LD) distance of 9.7 kb, with a significant SNP being identified within the <i>Zm00001d044327</i> candidate gene. RNA was then isolated from 12 different inbred maize lines from this GWAS study cohort and was used to conduct qPCR analyses which revealed significant differences in <i>Zm00001d044327</i> expression among strains exhibiting significant differences in leaf width. Based on an assessment of EMS mutant lines harboring a conserved amino acid stop mutation and two non-synonymous mutations in <i>Zm00001d044327</i> that exhibited a narrow leaf width, these data suggested that <i>Zm00001d044327</i> is a key regulator of maize leaf width. |
| format | Article |
| id | doaj-art-a0fde7ea03af4fccba63e9ddcecc84dc |
| institution | OA Journals |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-a0fde7ea03af4fccba63e9ddcecc84dc2025-08-20T01:55:37ZengMDPI AGLife2075-17292024-08-01149105710.3390/life14091057Screening and Validation of Leaf Width-Related Genes in Inbred Maize LinesShi Lu0Qi Wang1Junqi Yin2Shubo Zheng3Tingting Gao4Xudong Zhou5Jianxin Zhang6Yuexian Xing7Yingjie Ma8Min Wang9Delong Zhou10Ming Lu11Wenguo Liu12Piwu Wang13Zhijun Zhang14Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Jinong Hi-tech Inc., Ltd., Kemao Street, No.303, Gongzhuling 136100, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaCollege of Agronomy, Jilin Agricultural University, Xincheng Street, No.1288, Changchun 130118, ChinaJilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, ChinaLeaf width is a key determinant of planting density and photosynthetic efficiency. In an effort to determine which genes regulate maize plant leaf width, we performed a genome-wide association study (GWAS) of 1.49 × 106 single nucleotide polymorphisms (SNPs) in 80 sequenced backbone inbred maize lines in Jilin Province, China, based upon phenotypic leaf width data from two years. In total, 14 SNPs were identified as being significantly related to leaf width (<i>p</i> < 0.000001), with these SNPs being located on chromosomes 1, 2, 3, 5, 6, 7, 8, and 9. A total of five candidate genes were identified within a mean linkage disequilibrium (LD) distance of 9.7 kb, with a significant SNP being identified within the <i>Zm00001d044327</i> candidate gene. RNA was then isolated from 12 different inbred maize lines from this GWAS study cohort and was used to conduct qPCR analyses which revealed significant differences in <i>Zm00001d044327</i> expression among strains exhibiting significant differences in leaf width. Based on an assessment of EMS mutant lines harboring a conserved amino acid stop mutation and two non-synonymous mutations in <i>Zm00001d044327</i> that exhibited a narrow leaf width, these data suggested that <i>Zm00001d044327</i> is a key regulator of maize leaf width.https://www.mdpi.com/2075-1729/14/9/1057maize (<i>Zea mays</i> L.)leaf widthGWAS<i>Zm00001d044327</i>qPCR analysisEMS mutants |
| spellingShingle | Shi Lu Qi Wang Junqi Yin Shubo Zheng Tingting Gao Xudong Zhou Jianxin Zhang Yuexian Xing Yingjie Ma Min Wang Delong Zhou Ming Lu Wenguo Liu Piwu Wang Zhijun Zhang Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines Life maize (<i>Zea mays</i> L.) leaf width GWAS <i>Zm00001d044327</i> qPCR analysis EMS mutants |
| title | Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines |
| title_full | Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines |
| title_fullStr | Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines |
| title_full_unstemmed | Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines |
| title_short | Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines |
| title_sort | screening and validation of leaf width related genes in inbred maize lines |
| topic | maize (<i>Zea mays</i> L.) leaf width GWAS <i>Zm00001d044327</i> qPCR analysis EMS mutants |
| url | https://www.mdpi.com/2075-1729/14/9/1057 |
| work_keys_str_mv | AT shilu screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT qiwang screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT junqiyin screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT shubozheng screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT tingtinggao screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT xudongzhou screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT jianxinzhang screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT yuexianxing screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT yingjiema screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT minwang screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT delongzhou screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT minglu screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT wenguoliu screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT piwuwang screeningandvalidationofleafwidthrelatedgenesininbredmaizelines AT zhijunzhang screeningandvalidationofleafwidthrelatedgenesininbredmaizelines |