Alterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosis

Abstract Objective The aim of this study was to investigate the alteration of drug-resistant proteins in nasopharyngeal carcinoma (NPC) after intensity-modulated radiation therapy (IMRT) with or without chemotherapy and its correlation with clinicopathologic features and short-term prognosis. Method...

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Main Authors: GaoFeng Wang, Yuan Li
Format: Article
Language:English
Published: Springer 2025-05-01
Series:Discover Oncology
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Online Access:https://doi.org/10.1007/s12672-025-02677-y
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author GaoFeng Wang
Yuan Li
author_facet GaoFeng Wang
Yuan Li
author_sort GaoFeng Wang
collection DOAJ
description Abstract Objective The aim of this study was to investigate the alteration of drug-resistant proteins in nasopharyngeal carcinoma (NPC) after intensity-modulated radiation therapy (IMRT) with or without chemotherapy and its correlation with clinicopathologic features and short-term prognosis. Methods Eligible NPC patients receiving IMRT with or without chemotherapy were studied. Tumor tissues from patients before treatment and at 2/3 of IMRT were obtained, and drug resistance proteins, P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP), lung resistance protein (LRP), and glutathione S-transferase π (GST-π) were detected. Meanwhile, the clinicopathological data of the patients were collected and statistically analyzed. The short-term prognosis of the patients was observed and recorded, and the correlation between progression-free survival (PFS) and positive drug-resistant proteins was analyzed. Results Before treatment, the positive rates of P-gp, MRP, LRP, and GST-π among the 43 NPC patients were 30.23%, 16.28%, 20.93%, and 34.88%, respectively; after treatment, the positive rates of P-gp and MRP were 65.12% and 41.86%, respectively (P-gp, P < 0.001; MRP, P = 0.009). Significantly higher positive rates of P-gp were observed only after treatment than before treatment in the IMRT-alone group (n = 18) and IMRT/chemotherapy group (n = 25). The intensity of P-gp, MRP and GST-π positive expression was increased in patients treated with IMRT/chemotherapy compared to pre-treatment. No correlation between positive expression of drug-resistant proteins and lymphatic metastasis was observed. Patients with P-gp and GST-π positivity had a more pronounced decrease in PFS. Conclusion P-gp, MRP, and GST-π are increased in NPC patients after IMRT with or without chemotherapy and are closely associated with poor short-term prognosis of patients.
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spelling doaj-art-a0f5d71d2c014193bcf434e64d415b712025-08-20T03:48:18ZengSpringerDiscover Oncology2730-60112025-05-0116111210.1007/s12672-025-02677-yAlterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosisGaoFeng Wang0Yuan Li1Department of Otorhinolaryngology, The Fourth People’s Hospital of Lin’an DistrictDepartment of Otolaryngology, The Affiliated Hospital of Hangzhou Normal University (Hangzhou Second People’s Hospital)Abstract Objective The aim of this study was to investigate the alteration of drug-resistant proteins in nasopharyngeal carcinoma (NPC) after intensity-modulated radiation therapy (IMRT) with or without chemotherapy and its correlation with clinicopathologic features and short-term prognosis. Methods Eligible NPC patients receiving IMRT with or without chemotherapy were studied. Tumor tissues from patients before treatment and at 2/3 of IMRT were obtained, and drug resistance proteins, P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP), lung resistance protein (LRP), and glutathione S-transferase π (GST-π) were detected. Meanwhile, the clinicopathological data of the patients were collected and statistically analyzed. The short-term prognosis of the patients was observed and recorded, and the correlation between progression-free survival (PFS) and positive drug-resistant proteins was analyzed. Results Before treatment, the positive rates of P-gp, MRP, LRP, and GST-π among the 43 NPC patients were 30.23%, 16.28%, 20.93%, and 34.88%, respectively; after treatment, the positive rates of P-gp and MRP were 65.12% and 41.86%, respectively (P-gp, P < 0.001; MRP, P = 0.009). Significantly higher positive rates of P-gp were observed only after treatment than before treatment in the IMRT-alone group (n = 18) and IMRT/chemotherapy group (n = 25). The intensity of P-gp, MRP and GST-π positive expression was increased in patients treated with IMRT/chemotherapy compared to pre-treatment. No correlation between positive expression of drug-resistant proteins and lymphatic metastasis was observed. Patients with P-gp and GST-π positivity had a more pronounced decrease in PFS. Conclusion P-gp, MRP, and GST-π are increased in NPC patients after IMRT with or without chemotherapy and are closely associated with poor short-term prognosis of patients.https://doi.org/10.1007/s12672-025-02677-yIntensity-modulated radiation therapyChemotherapyDrug resistanceP-gpPrognosisGST-π
spellingShingle GaoFeng Wang
Yuan Li
Alterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosis
Discover Oncology
Intensity-modulated radiation therapy
Chemotherapy
Drug resistance
P-gp
Prognosis
GST-π
title Alterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosis
title_full Alterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosis
title_fullStr Alterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosis
title_full_unstemmed Alterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosis
title_short Alterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosis
title_sort alterations of drug resistant proteins in nasopharyngeal carcinoma after intensity modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short term prognosis
topic Intensity-modulated radiation therapy
Chemotherapy
Drug resistance
P-gp
Prognosis
GST-π
url https://doi.org/10.1007/s12672-025-02677-y
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AT yuanli alterationsofdrugresistantproteinsinnasopharyngealcarcinomaafterintensitymodulatedradiationtherapywithorwithoutchemotherapyandcorrelationwithclinicopathologicfeaturesandshorttermprognosis