LAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cells
Abstract Ovarian cancer is the most lethal tumor in female reproductive system, which seriously threatens the life and health of women. Our previous study found that LAMC3 was down-regulated in ovarian cancer tissues and correlated with ovarian cancer resistance. However, the effects of LAMC3 interf...
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| Format: | Article |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-07055-w |
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| author | Xia Liu Jun Zhang Lianping Xia Yuting Su Xiaoying Chen Cong Wang Yiqiang Ouyang Fuqiang Yin |
| author_facet | Xia Liu Jun Zhang Lianping Xia Yuting Su Xiaoying Chen Cong Wang Yiqiang Ouyang Fuqiang Yin |
| author_sort | Xia Liu |
| collection | DOAJ |
| description | Abstract Ovarian cancer is the most lethal tumor in female reproductive system, which seriously threatens the life and health of women. Our previous study found that LAMC3 was down-regulated in ovarian cancer tissues and correlated with ovarian cancer resistance. However, the effects of LAMC3 interference on drug resistance in carboplatin-resistant ovarian cancer cells remain unknown. In the present study, the effects of LAMC3 interference on drug resistance, cell proliferation, cycle, apoptosis, and ultrastructure of carboplatin-resistant ovarian cancer cells were examined. The results showed that LAMC3 interference lowered drug resistance of carboplatin-resistant ovarian cancer cells. The cell proliferation was inhibited and cell apoptosis was promoted in carboplatin-resistant ovarian cancer cells with LAMC3 interference. Besides, LAMC3 interference arrested cell cycle and affected cell ultrastructure of carboplatin-resistant ovarian cancer cells. Furthermore, transcriptome sequencing and WB verification results revealed cell cycle, autophagy, ferroptosis, lysosome related key pathway proteins may be involved in LAMC3 interference regulating drug resistance of carboplatin-resistant ovarian cancer cells. This study indicated LAMC3 interference reduced drug resistance of carboplatin-resistant ovarian cancer cells principal by affecting cell cycle, autophagy, ferroptosis, and lysosome. The present study provides a potential target for clinical treatment of drug resistance in ovarian cancer. |
| format | Article |
| id | doaj-art-a0f3a301ad0f4537a372655daa18900f |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
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| spelling | doaj-art-a0f3a301ad0f4537a372655daa18900f2025-08-20T04:01:26ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-07055-wLAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cellsXia Liu0Jun Zhang1Lianping Xia2Yuting Su3Xiaoying Chen4Cong Wang5Yiqiang Ouyang6Fuqiang Yin7Key Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry of Education, Centre for Translational Medicine, School of Preclinical Medicine, Guangxi Medical UniversityLaboratory Animal Center, Guangxi Medical UniversityKey Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry of Education, Centre for Translational Medicine, School of Preclinical Medicine, Guangxi Medical UniversityKey Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry of Education, Centre for Translational Medicine, School of Preclinical Medicine, Guangxi Medical UniversityKey Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry of Education, Centre for Translational Medicine, School of Preclinical Medicine, Guangxi Medical UniversityKey Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry of Education, Centre for Translational Medicine, School of Preclinical Medicine, Guangxi Medical UniversityLaboratory Animal Center, Guangxi Medical UniversityLife Science Institute, Guangxi Medical UniversityAbstract Ovarian cancer is the most lethal tumor in female reproductive system, which seriously threatens the life and health of women. Our previous study found that LAMC3 was down-regulated in ovarian cancer tissues and correlated with ovarian cancer resistance. However, the effects of LAMC3 interference on drug resistance in carboplatin-resistant ovarian cancer cells remain unknown. In the present study, the effects of LAMC3 interference on drug resistance, cell proliferation, cycle, apoptosis, and ultrastructure of carboplatin-resistant ovarian cancer cells were examined. The results showed that LAMC3 interference lowered drug resistance of carboplatin-resistant ovarian cancer cells. The cell proliferation was inhibited and cell apoptosis was promoted in carboplatin-resistant ovarian cancer cells with LAMC3 interference. Besides, LAMC3 interference arrested cell cycle and affected cell ultrastructure of carboplatin-resistant ovarian cancer cells. Furthermore, transcriptome sequencing and WB verification results revealed cell cycle, autophagy, ferroptosis, lysosome related key pathway proteins may be involved in LAMC3 interference regulating drug resistance of carboplatin-resistant ovarian cancer cells. This study indicated LAMC3 interference reduced drug resistance of carboplatin-resistant ovarian cancer cells principal by affecting cell cycle, autophagy, ferroptosis, and lysosome. The present study provides a potential target for clinical treatment of drug resistance in ovarian cancer.https://doi.org/10.1038/s41598-025-07055-wOvarian cancerCarboplatinDrug resistanceLAMC3 |
| spellingShingle | Xia Liu Jun Zhang Lianping Xia Yuting Su Xiaoying Chen Cong Wang Yiqiang Ouyang Fuqiang Yin LAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cells Scientific Reports Ovarian cancer Carboplatin Drug resistance LAMC3 |
| title | LAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cells |
| title_full | LAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cells |
| title_fullStr | LAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cells |
| title_full_unstemmed | LAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cells |
| title_short | LAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cells |
| title_sort | lamc3 interference reduces drug resistance of carboplatin resistant ovarian cancer cells |
| topic | Ovarian cancer Carboplatin Drug resistance LAMC3 |
| url | https://doi.org/10.1038/s41598-025-07055-w |
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