Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier
Intestinal dysbiosis is central to inflammatory bowel disease (IBD) development. Probiotics produce propionate, a short-chain fatty acid that restores intestinal balance and reduces inflammation.Comparative genomic analysis of propionate pathway integrity was performed to nominate 41 candidate strai...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000027 |
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| author | Ting Shu Xizi Song Silu Zhang Xiaoli Zhou Zhizhu Zhang Pengfei Wang Hu Canying Jie Pan Xiaoshuang Dai Yan Tan Wu Xiyang Huicui Meng Guoxun Xiao Huaijie Hao |
| author_facet | Ting Shu Xizi Song Silu Zhang Xiaoli Zhou Zhizhu Zhang Pengfei Wang Hu Canying Jie Pan Xiaoshuang Dai Yan Tan Wu Xiyang Huicui Meng Guoxun Xiao Huaijie Hao |
| author_sort | Ting Shu |
| collection | DOAJ |
| description | Intestinal dysbiosis is central to inflammatory bowel disease (IBD) development. Probiotics produce propionate, a short-chain fatty acid that restores intestinal balance and reduces inflammation.Comparative genomic analysis of propionate pathway integrity was performed to nominate 41 candidate strains. Metabolic analysis confirmed the propionate production of 9 strains of them. Caco-2/HT29-MTX-E12 confluence epithelial model was established to investigate the effect of the strains on intestinal cell. DSS-induced colitis in a mouse model was also included as an in vivo verification of the filtered probiotic strains.The methods successfully nominated 2 potential strains: Bifidobacterium breve XA-2103 and Pediococcus acidilactici GOLDGUT-PA0755. Both had a preferential ability to produce propionate and to reduce the effect on the intestinal barrier in the confluence cell model. Furthermore, their therapeutic effects were verified by reduced body weight loss, shortening of the colon length, Disease Activity Index score and improvement in histological analysis. |
| format | Article |
| id | doaj-art-a0ebb7d80a0344da8883fae558ee69da |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-a0ebb7d80a0344da8883fae558ee69da2025-02-09T04:59:53ZengElsevierJournal of Functional Foods1756-46462025-02-01125106660Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrierTing Shu0Xizi Song1Silu Zhang2Xiaoli Zhou3Zhizhu Zhang4Pengfei Wang5Hu Canying6Jie Pan7Xiaoshuang Dai8Yan Tan9Wu Xiyang10Huicui Meng11Guoxun Xiao12Huaijie Hao13Wonderlab Innovation Centre for Healthcare, Shenzhen Porshealth Bioengineering Co., Ltd, Shenzhen 518000, ChinaShenzhen Xbiome Biotech Co., Ltd., Shenzhen 518000, ChinaWonderlab Innovation Centre for Healthcare, Shenzhen Porshealth Bioengineering Co., Ltd, Shenzhen 518000, ChinaWonderlab Innovation Centre for Healthcare, Shenzhen Porshealth Bioengineering Co., Ltd, Shenzhen 518000, ChinaWonderlab Innovation Centre for Healthcare, Shenzhen Porshealth Bioengineering Co., Ltd, Shenzhen 518000, ChinaSun Yat-sen memorial hospital, Sun Yat-sen University, Guangzhou 510235, ChinaShenzhen Xbiome Biotech Co., Ltd., Shenzhen 518000, ChinaShenzhen Xbiome Biotech Co., Ltd., Shenzhen 518000, ChinaShenzhen Xbiome Biotech Co., Ltd., Shenzhen 518000, ChinaShenzhen Xbiome Biotech Co., Ltd., Shenzhen 518000, ChinaDepartment of Food Science and Engineering, Jinan University, Guangzhou 510632, ChinaSchool of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-Sen University, Shenzhen, Guangdong 518107, ChinaWonderlab Innovation Centre for Healthcare, Shenzhen Porshealth Bioengineering Co., Ltd, Shenzhen 518000, China; Corresponding authors.Shenzhen Xbiome Biotech Co., Ltd., Shenzhen 518000, China; Corresponding authors.Intestinal dysbiosis is central to inflammatory bowel disease (IBD) development. Probiotics produce propionate, a short-chain fatty acid that restores intestinal balance and reduces inflammation.Comparative genomic analysis of propionate pathway integrity was performed to nominate 41 candidate strains. Metabolic analysis confirmed the propionate production of 9 strains of them. Caco-2/HT29-MTX-E12 confluence epithelial model was established to investigate the effect of the strains on intestinal cell. DSS-induced colitis in a mouse model was also included as an in vivo verification of the filtered probiotic strains.The methods successfully nominated 2 potential strains: Bifidobacterium breve XA-2103 and Pediococcus acidilactici GOLDGUT-PA0755. Both had a preferential ability to produce propionate and to reduce the effect on the intestinal barrier in the confluence cell model. Furthermore, their therapeutic effects were verified by reduced body weight loss, shortening of the colon length, Disease Activity Index score and improvement in histological analysis.http://www.sciencedirect.com/science/article/pii/S1756464625000027Inflammatory bowel diseasePropionateProbiotics screeningCaco-2/HT29-MTX-E12 confluence epithelial modelBifidobacterium brevePediococcus acidilactici |
| spellingShingle | Ting Shu Xizi Song Silu Zhang Xiaoli Zhou Zhizhu Zhang Pengfei Wang Hu Canying Jie Pan Xiaoshuang Dai Yan Tan Wu Xiyang Huicui Meng Guoxun Xiao Huaijie Hao Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier Journal of Functional Foods Inflammatory bowel disease Propionate Probiotics screening Caco-2/HT29-MTX-E12 confluence epithelial model Bifidobacterium breve Pediococcus acidilactici |
| title | Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier |
| title_full | Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier |
| title_fullStr | Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier |
| title_full_unstemmed | Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier |
| title_short | Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier |
| title_sort | screen of propionate producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier |
| topic | Inflammatory bowel disease Propionate Probiotics screening Caco-2/HT29-MTX-E12 confluence epithelial model Bifidobacterium breve Pediococcus acidilactici |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000027 |
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