Screen of propionate-producing probiotic strains in attenuating murine colitis by modulating immune response and restoring the intestinal barrier
Intestinal dysbiosis is central to inflammatory bowel disease (IBD) development. Probiotics produce propionate, a short-chain fatty acid that restores intestinal balance and reduces inflammation.Comparative genomic analysis of propionate pathway integrity was performed to nominate 41 candidate strai...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000027 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Intestinal dysbiosis is central to inflammatory bowel disease (IBD) development. Probiotics produce propionate, a short-chain fatty acid that restores intestinal balance and reduces inflammation.Comparative genomic analysis of propionate pathway integrity was performed to nominate 41 candidate strains. Metabolic analysis confirmed the propionate production of 9 strains of them. Caco-2/HT29-MTX-E12 confluence epithelial model was established to investigate the effect of the strains on intestinal cell. DSS-induced colitis in a mouse model was also included as an in vivo verification of the filtered probiotic strains.The methods successfully nominated 2 potential strains: Bifidobacterium breve XA-2103 and Pediococcus acidilactici GOLDGUT-PA0755. Both had a preferential ability to produce propionate and to reduce the effect on the intestinal barrier in the confluence cell model. Furthermore, their therapeutic effects were verified by reduced body weight loss, shortening of the colon length, Disease Activity Index score and improvement in histological analysis. |
|---|---|
| ISSN: | 1756-4646 |