pSIG plasmids, MoClo-compatible vectors for efficient production of chimeric double-stranded RNAs in Escherichia coli HT115 (DE3) strain

pSIG plasmids, MoClo-compatible vectors for efficient production of chimeric double-stranded RNAs in Escherichia coli HT115 (DE3) strain

Abstract Background Spray-induced gene silencing (SIGS) is a promising strategy for controlling plant diseases caused by pests, fungi, and viruses. The method involves spraying on plant surfaces double-stranded RNAs (dsRNAs) that target pathogen genes and inhibit pathogen growth via activation of th...

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Bibliographic Details
Main Authors: Ching-Feng Wu, Li-Pang Chang, Chan Lee, Ioannis Stergiopoulos, Li-Hung Chen
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Plant Methods
Subjects:
RNA interference
Double-stranded RNA
In vivo dsRNA production
Botrytis cinerea
Escherichia coli HT115 (DE3)
Online Access:https://doi.org/10.1186/s13007-025-01413-5
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