Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats
In the present study, we investigated the potential immunomodulatory effects of heat-killed (hLR) and live Limosilactobacillus reuteri PSC102 (LR; formerly Lactobacillus reuteri PSC102) in RAW264.7 macrophage cells and Sprague–Dawley rats. RAW264.7 murine macrophage cells were stimulated with hLR an...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Veterinary Quarterly |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/01652176.2024.2344765 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850060311977000960 |
|---|---|
| author | Md. Sekendar Ali Eon-Bee Lee Yixian Quah Syed Al Jawad Sayem Muhammad Aleem Abbas Kyoungho Suk Seung-Jin Lee Seung-Chun Park |
| author_facet | Md. Sekendar Ali Eon-Bee Lee Yixian Quah Syed Al Jawad Sayem Muhammad Aleem Abbas Kyoungho Suk Seung-Jin Lee Seung-Chun Park |
| author_sort | Md. Sekendar Ali |
| collection | DOAJ |
| description | In the present study, we investigated the potential immunomodulatory effects of heat-killed (hLR) and live Limosilactobacillus reuteri PSC102 (LR; formerly Lactobacillus reuteri PSC102) in RAW264.7 macrophage cells and Sprague–Dawley rats. RAW264.7 murine macrophage cells were stimulated with hLR and LR for 24 h. Cyclophosphamide (CTX)-induced immunosuppressed Sprague–Dawley rats were orally administered with three doses of hLR (L-Low, M-Medium, and H-High) and LR for 3 weeks. The phagocytic capacity, production of nitric oxide (NO), and expression of cytokines in RAW264.7 cells were measured, and the different parameters of immunity in rats were determined. hLR and LR treatments promoted phagocytic activity and induced the production of NO and the expression of iNOS, TNF-α, IL-1β, IL-6, and Cox-2 in macrophage cells. In the in vivo experiment, hLR and LR treatments significantly increased the immune organ indices, alleviated the spleen injury, and ameliorated the number of white blood cells, granulocytes, lymphocytes, and mid-range absolute counts in immunosuppressive rats. hLR and LR increased neutrophil migration and phagocytosis, splenocyte proliferation, and T lymphocyte subsets (CD4+, CD8+, CD45RA+, and CD28+). The levels of immune factors (IL-2, IL-4, IL-6, IL-10, IL-12A, TNF-α, and IFN-γ) in the hLR and LR groups were upregulated compared with those in the CTX-treatment group. hLR and LR treatments could also modulate the gut microbiota composition, thereby increasing the relative abundance of Bacteroidetes and Firmicutes but decreasing the level of Proteobacteria. hLR and LR protected against CTX-induced adverse reactions by modulating the immune response and gut microbiota composition. Therefore, they could be used as potential immunomodulatory agents. |
| format | Article |
| id | doaj-art-a0cda1c797f142f48c77383e24fcfe82 |
| institution | DOAJ |
| issn | 0165-2176 1875-5941 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj-art-a0cda1c797f142f48c77383e24fcfe822025-08-20T02:50:37ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412024-12-0144111810.1080/01652176.2024.2344765Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated ratsMd. Sekendar Ali0Eon-Bee Lee1Yixian Quah2Syed Al Jawad Sayem3Muhammad Aleem Abbas4Kyoungho Suk5Seung-Jin Lee6Seung-Chun Park7Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaLaboratory of Veterinary Pharmacokinetics and Pharmacodynamics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaDevelopmental and Reproductive Toxicology Research Group, Korea Institute of Toxicology, Daejeon, South KoreaLaboratory of Veterinary Pharmacokinetics and Pharmacodynamics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaLaboratory of Veterinary Pharmacokinetics and Pharmacodynamics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Biomedical Science and Department of Pharmacology, School of Medicine, Brain Science and Engineering Institute, Kyungpook National University, Daegu, South KoreaDevelopmental and Reproductive Toxicology Research Group, Korea Institute of Toxicology, Daejeon, South KoreaLaboratory of Veterinary Pharmacokinetics and Pharmacodynamics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaIn the present study, we investigated the potential immunomodulatory effects of heat-killed (hLR) and live Limosilactobacillus reuteri PSC102 (LR; formerly Lactobacillus reuteri PSC102) in RAW264.7 macrophage cells and Sprague–Dawley rats. RAW264.7 murine macrophage cells were stimulated with hLR and LR for 24 h. Cyclophosphamide (CTX)-induced immunosuppressed Sprague–Dawley rats were orally administered with three doses of hLR (L-Low, M-Medium, and H-High) and LR for 3 weeks. The phagocytic capacity, production of nitric oxide (NO), and expression of cytokines in RAW264.7 cells were measured, and the different parameters of immunity in rats were determined. hLR and LR treatments promoted phagocytic activity and induced the production of NO and the expression of iNOS, TNF-α, IL-1β, IL-6, and Cox-2 in macrophage cells. In the in vivo experiment, hLR and LR treatments significantly increased the immune organ indices, alleviated the spleen injury, and ameliorated the number of white blood cells, granulocytes, lymphocytes, and mid-range absolute counts in immunosuppressive rats. hLR and LR increased neutrophil migration and phagocytosis, splenocyte proliferation, and T lymphocyte subsets (CD4+, CD8+, CD45RA+, and CD28+). The levels of immune factors (IL-2, IL-4, IL-6, IL-10, IL-12A, TNF-α, and IFN-γ) in the hLR and LR groups were upregulated compared with those in the CTX-treatment group. hLR and LR treatments could also modulate the gut microbiota composition, thereby increasing the relative abundance of Bacteroidetes and Firmicutes but decreasing the level of Proteobacteria. hLR and LR protected against CTX-induced adverse reactions by modulating the immune response and gut microbiota composition. Therefore, they could be used as potential immunomodulatory agents.https://www.tandfonline.com/doi/10.1080/01652176.2024.2344765Limosilactobacillus reuteri PSC102neutrophil migration and phagocytosissplenocyte proliferationT lymphocyte differentiationcytokinesgut microbiota |
| spellingShingle | Md. Sekendar Ali Eon-Bee Lee Yixian Quah Syed Al Jawad Sayem Muhammad Aleem Abbas Kyoungho Suk Seung-Jin Lee Seung-Chun Park Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats Veterinary Quarterly Limosilactobacillus reuteri PSC102 neutrophil migration and phagocytosis splenocyte proliferation T lymphocyte differentiation cytokines gut microbiota |
| title | Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats |
| title_full | Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats |
| title_fullStr | Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats |
| title_full_unstemmed | Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats |
| title_short | Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats |
| title_sort | modulating effects of heat killed and live limosilactobacillus reuteri psc102 on the immune response and gut microbiota of cyclophosphamide treated rats |
| topic | Limosilactobacillus reuteri PSC102 neutrophil migration and phagocytosis splenocyte proliferation T lymphocyte differentiation cytokines gut microbiota |
| url | https://www.tandfonline.com/doi/10.1080/01652176.2024.2344765 |
| work_keys_str_mv | AT mdsekendarali modulatingeffectsofheatkilledandlivelimosilactobacillusreuteripsc102ontheimmuneresponseandgutmicrobiotaofcyclophosphamidetreatedrats AT eonbeelee modulatingeffectsofheatkilledandlivelimosilactobacillusreuteripsc102ontheimmuneresponseandgutmicrobiotaofcyclophosphamidetreatedrats AT yixianquah modulatingeffectsofheatkilledandlivelimosilactobacillusreuteripsc102ontheimmuneresponseandgutmicrobiotaofcyclophosphamidetreatedrats AT syedaljawadsayem modulatingeffectsofheatkilledandlivelimosilactobacillusreuteripsc102ontheimmuneresponseandgutmicrobiotaofcyclophosphamidetreatedrats AT muhammadaleemabbas modulatingeffectsofheatkilledandlivelimosilactobacillusreuteripsc102ontheimmuneresponseandgutmicrobiotaofcyclophosphamidetreatedrats AT kyounghosuk modulatingeffectsofheatkilledandlivelimosilactobacillusreuteripsc102ontheimmuneresponseandgutmicrobiotaofcyclophosphamidetreatedrats AT seungjinlee modulatingeffectsofheatkilledandlivelimosilactobacillusreuteripsc102ontheimmuneresponseandgutmicrobiotaofcyclophosphamidetreatedrats AT seungchunpark modulatingeffectsofheatkilledandlivelimosilactobacillusreuteripsc102ontheimmuneresponseandgutmicrobiotaofcyclophosphamidetreatedrats |