Grapevine dieback caused by Botryosphaeriaceae species, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata in Piedmont: characterization and pathogenicity
Grapevine Trunk Diseases (GTDs) are major threats in Mediterranean countries, causing economic losses due to reduced grape yields and long-term vine productivity, as well as death of grapevines. A survey was conducted in Piedmont (Northern Italy) during 2021-2022 to investigate the species diversity...
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Firenze University Press
2023-09-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/14673 |
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| author | Greta DARDANI Laura MUGNAI Simone BUSSOTTI M. Lodovica GULLINO Vladimiro GUARNACCIA |
| author_facet | Greta DARDANI Laura MUGNAI Simone BUSSOTTI M. Lodovica GULLINO Vladimiro GUARNACCIA |
| author_sort | Greta DARDANI |
| collection | DOAJ |
| description | Grapevine Trunk Diseases (GTDs) are major threats in Mediterranean countries, causing economic losses due to reduced grape yields and long-term vine productivity, as well as death of grapevines. A survey was conducted in Piedmont (Northern Italy) during 2021-2022 to investigate the species diversity and distribution of GTD pathogens in this important Italian wine region. Morphological and multi-locus phylogenetic analyses (based on ITS, tef1, tub2, act and rpb2) identified species of Botryosphaeriaceae at high frequency, including Botryosphaeria dothidea, Diplodia mutila, Diplodia seriata and Neofusicoccum parvum. Other pathogens commonly associated with GTDs, including Eutypa lata, Fomitiporia mediterranea and Phaeomoniella chlamydospora, were also isolated. Less commonly isolated species included Neocucurbitaria juglandicola, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata. Pathogenicity tests with two representative isolates of each species were carried out using one-year-old potted grapevine cuttings (‘Barbera’). All isolates (except N. juglandicola) caused brown wood necrotic vascular discolourations on inoculated plants and were successfully re-isolated. Effects of temperature on colony growth were also assessed. For all tested isolates there was no growth at 5°C, only four isolates (Botryosphaeriaceae) grew at 35°C, and optimum growth temperatures were between 20 and 25°C. This is the first record of Paraconiothyrium brasiliense and Neocucurbitaria juglandicola associated with symptomatic grapevines in Italy. |
| format | Article |
| id | doaj-art-a0cbf40810ce49d080bde5e3f8f3cfb1 |
| institution | DOAJ |
| issn | 0031-9465 1593-2095 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj-art-a0cbf40810ce49d080bde5e3f8f3cfb12025-08-20T03:05:25ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952023-09-0162228330610.36253/phyto-1467313502Grapevine dieback caused by Botryosphaeriaceae species, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata in Piedmont: characterization and pathogenicityGreta DARDANI0https://orcid.org/0000-0002-9146-2168Laura MUGNAI1https://orcid.org/0000-0002-2508-9764Simone BUSSOTTI2M. Lodovica GULLINO3https://orcid.org/0000-0002-7706-1915Vladimiro GUARNACCIA4https://orcid.org/0000-0003-3188-7743Centre for Innovation in the Agro-Environmental Sector, AGROINNOVA, University of Torino, Largo Braccini 2, 10095 Grugliasco (TO)Department of Agricultural, Food, Environmental and Forestry Science and Technology (DAGRI), Plant Pathology and Entomology Section, University of Florence, P.le delle Cascine, 28, 50144 FirenzeAGRION, The Foundation for Research, Innovation and Technological Development of Piedmont Agriculture, 12030 Manta (CN)Centre for Innovation in the Agro-Environmental Sector, AGROINNOVA, University of Torino, Largo Braccini 2, 10095 Grugliasco (TO)Centre for Innovation in the Agro-Environmental Sector, AGROINNOVA, University of Torino, Largo Braccini 2, 10095 Grugliasco (TO)Grapevine Trunk Diseases (GTDs) are major threats in Mediterranean countries, causing economic losses due to reduced grape yields and long-term vine productivity, as well as death of grapevines. A survey was conducted in Piedmont (Northern Italy) during 2021-2022 to investigate the species diversity and distribution of GTD pathogens in this important Italian wine region. Morphological and multi-locus phylogenetic analyses (based on ITS, tef1, tub2, act and rpb2) identified species of Botryosphaeriaceae at high frequency, including Botryosphaeria dothidea, Diplodia mutila, Diplodia seriata and Neofusicoccum parvum. Other pathogens commonly associated with GTDs, including Eutypa lata, Fomitiporia mediterranea and Phaeomoniella chlamydospora, were also isolated. Less commonly isolated species included Neocucurbitaria juglandicola, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata. Pathogenicity tests with two representative isolates of each species were carried out using one-year-old potted grapevine cuttings (‘Barbera’). All isolates (except N. juglandicola) caused brown wood necrotic vascular discolourations on inoculated plants and were successfully re-isolated. Effects of temperature on colony growth were also assessed. For all tested isolates there was no growth at 5°C, only four isolates (Botryosphaeriaceae) grew at 35°C, and optimum growth temperatures were between 20 and 25°C. This is the first record of Paraconiothyrium brasiliense and Neocucurbitaria juglandicola associated with symptomatic grapevines in Italy.https://oajournals.fupress.net/index.php/pm/article/view/14673botryosphaeria diebackdiplodia mutilaneocucurbitaria juglandicolapathogenicitygrapevine trunk diseases |
| spellingShingle | Greta DARDANI Laura MUGNAI Simone BUSSOTTI M. Lodovica GULLINO Vladimiro GUARNACCIA Grapevine dieback caused by Botryosphaeriaceae species, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata in Piedmont: characterization and pathogenicity Phytopathologia Mediterranea botryosphaeria dieback diplodia mutila neocucurbitaria juglandicola pathogenicity grapevine trunk diseases |
| title | Grapevine dieback caused by Botryosphaeriaceae species, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata in Piedmont: characterization and pathogenicity |
| title_full | Grapevine dieback caused by Botryosphaeriaceae species, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata in Piedmont: characterization and pathogenicity |
| title_fullStr | Grapevine dieback caused by Botryosphaeriaceae species, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata in Piedmont: characterization and pathogenicity |
| title_full_unstemmed | Grapevine dieback caused by Botryosphaeriaceae species, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata in Piedmont: characterization and pathogenicity |
| title_short | Grapevine dieback caused by Botryosphaeriaceae species, Paraconiothyrium brasiliense, Seimatosporium vitis-viniferae and Truncatella angustata in Piedmont: characterization and pathogenicity |
| title_sort | grapevine dieback caused by botryosphaeriaceae species paraconiothyrium brasiliense seimatosporium vitis viniferae and truncatella angustata in piedmont characterization and pathogenicity |
| topic | botryosphaeria dieback diplodia mutila neocucurbitaria juglandicola pathogenicity grapevine trunk diseases |
| url | https://oajournals.fupress.net/index.php/pm/article/view/14673 |
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