Diamond-like carbon films over reconstructive TMJ prosthetic materials: Effects in the cytotoxicity, chemical and mechanical properties

Increasingly more young patients have been submitted to reconstruction of the Temporomandibular Joint (TMJ), so, the prostheses must to present more functional longevity. Objective: To evaluate the effect of diamond-like carbon film (DLC) over titanium alloy (Ti6Al4V) and polyethylene (UHWPE) sample...

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Main Authors: Alecsandro de Moura Silva, Viviane Maria Gonçalves de Figueiredo, Renata Falchete do Prado, Gabriela de Fátima Santanta-Melo, Milagros del Valle El Abras Ankha, Luana Marotta Reis de Vasconcellos, Argemiro Soares da Silva Sobrinho, Alexandre Luiz Souto Borges, Lafayette Nogueira Junior
Format: Article
Language:English
Published: Elsevier 2019-07-01
Series:Journal of Oral Biology and Craniofacial Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2212426819300892
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author Alecsandro de Moura Silva
Viviane Maria Gonçalves de Figueiredo
Renata Falchete do Prado
Gabriela de Fátima Santanta-Melo
Milagros del Valle El Abras Ankha
Luana Marotta Reis de Vasconcellos
Argemiro Soares da Silva Sobrinho
Alexandre Luiz Souto Borges
Lafayette Nogueira Junior
author_facet Alecsandro de Moura Silva
Viviane Maria Gonçalves de Figueiredo
Renata Falchete do Prado
Gabriela de Fátima Santanta-Melo
Milagros del Valle El Abras Ankha
Luana Marotta Reis de Vasconcellos
Argemiro Soares da Silva Sobrinho
Alexandre Luiz Souto Borges
Lafayette Nogueira Junior
author_sort Alecsandro de Moura Silva
collection DOAJ
description Increasingly more young patients have been submitted to reconstruction of the Temporomandibular Joint (TMJ), so, the prostheses must to present more functional longevity. Objective: To evaluate the effect of diamond-like carbon film (DLC) over titanium alloy (Ti6Al4V) and polyethylene (UHWPE) samples, their mechanical and chemical properties and cellular cytotoxicity. Methods: Titanium and UHWPE specimens, with 2.5 cm in diameter and 2 mm thickness were coated through plasma enhanced chemical vapor deposition (PECVD) with DLC or DLC doped with silver (DLC-Ag). Scanning electron microscopy (SEM) morphological analysis, Energy-dispersive spectroscopy (EDS) chemical analysis, scratching test, mechanical fatigue test, surface roughness analysis, and cellular cytotoxicity were performed. Data were statistically analyzed using one-way ANOVA (p < 0.05) or two-way ANOVA and multiple comparison Tukey test. Results: In the SEM analysis, morphological differences were observed on substrates after DLC deposition. The film chemically modified the substrate surfaces, according to the EDS analysis. The initial critical load failure occurred at 6.1 N for DLC and 9.7 N for the DLC-Ag film. The DLC film deposition over the polyethylene promoted a decrease in the polymer's damaged area after mechanical fatigue cycling. The cytotoxicity analysis demonstrated less biocompatibility in experimental groups, when compared to control, however, increased biocompatibility was observed, at 10 days, in all groups. Conclusion: The diamond-like carbon coating enhanced the chemical and mechanical properties from substrates, however modified biological interaction course of the titanium alloy (Ti6Al4V) and polyethylene (UHWPE) samples. Parameters for film deposition remain to be improved in order to obtain best biocompatibility.
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spelling doaj-art-a0c5b0b9087d47e1b867e2a0c52498392024-11-23T06:27:48ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682019-07-0193201207Diamond-like carbon films over reconstructive TMJ prosthetic materials: Effects in the cytotoxicity, chemical and mechanical propertiesAlecsandro de Moura Silva0Viviane Maria Gonçalves de Figueiredo1Renata Falchete do Prado2Gabriela de Fátima Santanta-Melo3Milagros del Valle El Abras Ankha4Luana Marotta Reis de Vasconcellos5Argemiro Soares da Silva Sobrinho6Alexandre Luiz Souto Borges7Lafayette Nogueira Junior8Department of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Sao Jose dos Campos, BrazilDepartment of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Sao Jose dos Campos, BrazilDepartment of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Sao Jose dos Campos, BrazilDepartment of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University - UNESP, BrazilDepartment of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University - UNESP, BrazilDepartment of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University - UNESP, BrazilDepartment of Physic, Technical Institute Aerospace (ITA), Sao Jose dos Campos, BrazilDepartment of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Sao Jose dos Campos, BrazilDepartment of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Sao Jose dos Campos, Brazil; Corresponding author. Department of Dental Materials and Prosthodontics, Institute of Science and Technology of São José dos Campos, São Paulo State University (UNESP), Avenida Engenheiro Francisco Jose Longo - Jardim Sao Dimas, 777, 12245-000, Sao Jose dos Campos - SP, Brazil.Increasingly more young patients have been submitted to reconstruction of the Temporomandibular Joint (TMJ), so, the prostheses must to present more functional longevity. Objective: To evaluate the effect of diamond-like carbon film (DLC) over titanium alloy (Ti6Al4V) and polyethylene (UHWPE) samples, their mechanical and chemical properties and cellular cytotoxicity. Methods: Titanium and UHWPE specimens, with 2.5 cm in diameter and 2 mm thickness were coated through plasma enhanced chemical vapor deposition (PECVD) with DLC or DLC doped with silver (DLC-Ag). Scanning electron microscopy (SEM) morphological analysis, Energy-dispersive spectroscopy (EDS) chemical analysis, scratching test, mechanical fatigue test, surface roughness analysis, and cellular cytotoxicity were performed. Data were statistically analyzed using one-way ANOVA (p < 0.05) or two-way ANOVA and multiple comparison Tukey test. Results: In the SEM analysis, morphological differences were observed on substrates after DLC deposition. The film chemically modified the substrate surfaces, according to the EDS analysis. The initial critical load failure occurred at 6.1 N for DLC and 9.7 N for the DLC-Ag film. The DLC film deposition over the polyethylene promoted a decrease in the polymer's damaged area after mechanical fatigue cycling. The cytotoxicity analysis demonstrated less biocompatibility in experimental groups, when compared to control, however, increased biocompatibility was observed, at 10 days, in all groups. Conclusion: The diamond-like carbon coating enhanced the chemical and mechanical properties from substrates, however modified biological interaction course of the titanium alloy (Ti6Al4V) and polyethylene (UHWPE) samples. Parameters for film deposition remain to be improved in order to obtain best biocompatibility.http://www.sciencedirect.com/science/article/pii/S2212426819300892TitaniumPolyethylenePlasma enhanced chemical vapor depositionDiamond like carbon
spellingShingle Alecsandro de Moura Silva
Viviane Maria Gonçalves de Figueiredo
Renata Falchete do Prado
Gabriela de Fátima Santanta-Melo
Milagros del Valle El Abras Ankha
Luana Marotta Reis de Vasconcellos
Argemiro Soares da Silva Sobrinho
Alexandre Luiz Souto Borges
Lafayette Nogueira Junior
Diamond-like carbon films over reconstructive TMJ prosthetic materials: Effects in the cytotoxicity, chemical and mechanical properties
Journal of Oral Biology and Craniofacial Research
Titanium
Polyethylene
Plasma enhanced chemical vapor deposition
Diamond like carbon
title Diamond-like carbon films over reconstructive TMJ prosthetic materials: Effects in the cytotoxicity, chemical and mechanical properties
title_full Diamond-like carbon films over reconstructive TMJ prosthetic materials: Effects in the cytotoxicity, chemical and mechanical properties
title_fullStr Diamond-like carbon films over reconstructive TMJ prosthetic materials: Effects in the cytotoxicity, chemical and mechanical properties
title_full_unstemmed Diamond-like carbon films over reconstructive TMJ prosthetic materials: Effects in the cytotoxicity, chemical and mechanical properties
title_short Diamond-like carbon films over reconstructive TMJ prosthetic materials: Effects in the cytotoxicity, chemical and mechanical properties
title_sort diamond like carbon films over reconstructive tmj prosthetic materials effects in the cytotoxicity chemical and mechanical properties
topic Titanium
Polyethylene
Plasma enhanced chemical vapor deposition
Diamond like carbon
url http://www.sciencedirect.com/science/article/pii/S2212426819300892
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