Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine
Cryopreservation plays a critical role in animal breeding and the conservation of endangered species, but it often compromises sperm characteristics such as morphology, motility, and viability due to oxidative stress. This study explores the antioxidative effect of Mito-Tempo (MT) and Berberine (BER...
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MDPI AG
2024-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/13/11/1360 |
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| author | Lenka Kuželová Andrea Svoradová Andrej Baláži Jaromír Vašíček Vladimír Langraf Adriana Kolesárová Petr Sláma Peter Chrenek |
| author_facet | Lenka Kuželová Andrea Svoradová Andrej Baláži Jaromír Vašíček Vladimír Langraf Adriana Kolesárová Petr Sláma Peter Chrenek |
| author_sort | Lenka Kuželová |
| collection | DOAJ |
| description | Cryopreservation plays a critical role in animal breeding and the conservation of endangered species, but it often compromises sperm characteristics such as morphology, motility, and viability due to oxidative stress. This study explores the antioxidative effect of Mito-Tempo (MT) and Berberine (BER) to enhance post-thaw sperm quality in rabbits. Pooled rabbit sperm samples were supplemented with different concentrations (0.0, 0.5, 5, 10, 50 µmol/L) of MT and BER. Sperm motility was evaluated using computer-assisted semen analysis, while viability, apoptosis, reactive oxygen species (ROS) levels, acrosome integrity, and mitochondrial function were assessed through flow cytometry. The results revealed that MT at 5 and 10 µmol/L and BER at 10 µmol/L significantly improved total and progressive motility, mitochondrial activity, and sperm viability compared to the control group. Furthermore, 10 µmol/L BER enhanced acrosome integrity, while both 5 µmol/L MT and 10 µmol/L BER effectively reduced ROS levels and apoptosis. This study is the first to demonstrate the protective effects of MT and BER on rabbit sperm during cryopreservation. By mitigating oxidative stress and reducing apoptosis, these antioxidants markedly improved post-thaw sperm quality, positioning MT and BER as promising agents for improving sperm cryosurvival. |
| format | Article |
| id | doaj-art-a0bfa5ec53a34b4f8dad04b162dd482f |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-a0bfa5ec53a34b4f8dad04b162dd482f2025-08-20T02:08:00ZengMDPI AGAntioxidants2076-39212024-11-011311136010.3390/antiox13111360Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and BerberineLenka Kuželová0Andrea Svoradová1Andrej Baláži2Jaromír Vašíček3Vladimír Langraf4Adriana Kolesárová5Petr Sláma6Peter Chrenek7Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Hlohovecká 2, 951 41 Lužianky, SlovakiaResearch Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Hlohovecká 2, 951 41 Lužianky, SlovakiaResearch Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Hlohovecká 2, 951 41 Lužianky, SlovakiaResearch Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Hlohovecká 2, 951 41 Lužianky, SlovakiaDepartment of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Tr. A. Hlinku 1, 949 01 Nitra, SlovakiaAgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech RepublicResearch Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Hlohovecká 2, 951 41 Lužianky, SlovakiaCryopreservation plays a critical role in animal breeding and the conservation of endangered species, but it often compromises sperm characteristics such as morphology, motility, and viability due to oxidative stress. This study explores the antioxidative effect of Mito-Tempo (MT) and Berberine (BER) to enhance post-thaw sperm quality in rabbits. Pooled rabbit sperm samples were supplemented with different concentrations (0.0, 0.5, 5, 10, 50 µmol/L) of MT and BER. Sperm motility was evaluated using computer-assisted semen analysis, while viability, apoptosis, reactive oxygen species (ROS) levels, acrosome integrity, and mitochondrial function were assessed through flow cytometry. The results revealed that MT at 5 and 10 µmol/L and BER at 10 µmol/L significantly improved total and progressive motility, mitochondrial activity, and sperm viability compared to the control group. Furthermore, 10 µmol/L BER enhanced acrosome integrity, while both 5 µmol/L MT and 10 µmol/L BER effectively reduced ROS levels and apoptosis. This study is the first to demonstrate the protective effects of MT and BER on rabbit sperm during cryopreservation. By mitigating oxidative stress and reducing apoptosis, these antioxidants markedly improved post-thaw sperm quality, positioning MT and BER as promising agents for improving sperm cryosurvival.https://www.mdpi.com/2076-3921/13/11/1360rabbitsperm cryopreservationMito-Tempoberberinesperm qualityoxidative stress |
| spellingShingle | Lenka Kuželová Andrea Svoradová Andrej Baláži Jaromír Vašíček Vladimír Langraf Adriana Kolesárová Petr Sláma Peter Chrenek Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine Antioxidants rabbit sperm cryopreservation Mito-Tempo berberine sperm quality oxidative stress |
| title | Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine |
| title_full | Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine |
| title_fullStr | Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine |
| title_full_unstemmed | Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine |
| title_short | Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine |
| title_sort | enhancing of rabbit sperm cryopreservation with antioxidants mito tempo and berberine |
| topic | rabbit sperm cryopreservation Mito-Tempo berberine sperm quality oxidative stress |
| url | https://www.mdpi.com/2076-3921/13/11/1360 |
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