Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

In the present study, a total of 1102304 serum samples were collected to detected human brucellosis between the years 2012 and 2016 in Inner Mongolia. Overall, an average of 3.79% anti-Brucella positive in Inner Mongolia was presented but the range of positive rates were among 0.90 to 7.07% in 12 re...

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Main Authors: Zhi-guo Liu, Miao Wang, Na Ta, Meng-gang Fang, Jing-chuan Mi, Rui-ping Yu, Yao Luo, Xiaoan Cao, Zhen-jun Li
Format: Article
Language:English
Published: Taylor & Francis Group 2020-01-01
Series:Emerging Microbes and Infections
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Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2020.1720528
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author Zhi-guo Liu
Miao Wang
Na Ta
Meng-gang Fang
Jing-chuan Mi
Rui-ping Yu
Yao Luo
Xiaoan Cao
Zhen-jun Li
author_facet Zhi-guo Liu
Miao Wang
Na Ta
Meng-gang Fang
Jing-chuan Mi
Rui-ping Yu
Yao Luo
Xiaoan Cao
Zhen-jun Li
author_sort Zhi-guo Liu
collection DOAJ
description In the present study, a total of 1102304 serum samples were collected to detected human brucellosis between the years 2012 and 2016 in Inner Mongolia. Overall, an average of 3.79% anti-Brucella positive in Inner Mongolia was presented but the range of positive rates were among 0.90 to 7.07% in 12 regions. Seroprevalence of human brucellosis increased gradually from 2012 to 2016. However, the incidence rate of human brucellosis showed a declining trend. One hundred and seven Brucella strains were isolated and identified as B. melitensis species, and B. melitensis biovar 3 was the predominant biovar. MLVA-11 genotypes 116 was predominant and had crucial epidemiology to the human population. All 107 strains tested were sorted into 75 MLVA-16 genotypes, with 54 single genotypes representing unique isolates. This result revealed that these Brucellosis cases had epidemiologically unrelated and sporadic characteristics. The remaining 21 shared genotypes among two to four strains, confirming the occurrence of cross-infection and multiple outbreaks. Extensive genotype-events were observed between strains from this study and Kazakhstan, Mongolia, and Turkey, these countries were key members of the grassland silk road. Long-time trade in small ruminants (sheep) in these countries has possibly promoted the spread of Brucella spp. in these regions.
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spelling doaj-art-a0bb595d2dd14a01beef3d718091e3702025-08-20T03:08:32ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019126327410.1080/22221751.2020.1720528Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016Zhi-guo Liu0Miao Wang1Na Ta2Meng-gang Fang3Jing-chuan Mi4Rui-ping Yu5Yao Luo6Xiaoan Cao7Zhen-jun Li8State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaUlanqab Centre for Endemic Disease Prevention and Control, Jining, Inner MongoliaInner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Huhhot, People’s Republic of ChinaInner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Huhhot, People’s Republic of ChinaInner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Huhhot, People’s Republic of ChinaInner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Huhhot, People’s Republic of ChinaFarmer School of Business, Miami University, Oxford, OH, USAState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, People’s Republic of ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of ChinaIn the present study, a total of 1102304 serum samples were collected to detected human brucellosis between the years 2012 and 2016 in Inner Mongolia. Overall, an average of 3.79% anti-Brucella positive in Inner Mongolia was presented but the range of positive rates were among 0.90 to 7.07% in 12 regions. Seroprevalence of human brucellosis increased gradually from 2012 to 2016. However, the incidence rate of human brucellosis showed a declining trend. One hundred and seven Brucella strains were isolated and identified as B. melitensis species, and B. melitensis biovar 3 was the predominant biovar. MLVA-11 genotypes 116 was predominant and had crucial epidemiology to the human population. All 107 strains tested were sorted into 75 MLVA-16 genotypes, with 54 single genotypes representing unique isolates. This result revealed that these Brucellosis cases had epidemiologically unrelated and sporadic characteristics. The remaining 21 shared genotypes among two to four strains, confirming the occurrence of cross-infection and multiple outbreaks. Extensive genotype-events were observed between strains from this study and Kazakhstan, Mongolia, and Turkey, these countries were key members of the grassland silk road. Long-time trade in small ruminants (sheep) in these countries has possibly promoted the spread of Brucella spp. in these regions.https://www.tandfonline.com/doi/10.1080/22221751.2020.1720528SerologyBrucella melitensisMLVAmolecular epidemiologyInner Mongolia
spellingShingle Zhi-guo Liu
Miao Wang
Na Ta
Meng-gang Fang
Jing-chuan Mi
Rui-ping Yu
Yao Luo
Xiaoan Cao
Zhen-jun Li
Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016
Emerging Microbes and Infections
Serology
Brucella melitensis
MLVA
molecular epidemiology
Inner Mongolia
title Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016
title_full Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016
title_fullStr Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016
title_full_unstemmed Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016
title_short Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016
title_sort seroprevalence of human brucellosis and molecular characteristics of brucella strains in inner mongolia autonomous region of china from 2012 to 2016
topic Serology
Brucella melitensis
MLVA
molecular epidemiology
Inner Mongolia
url https://www.tandfonline.com/doi/10.1080/22221751.2020.1720528
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