A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine
Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/9404068 |
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| author | Imran Khan K. M. Taufiqur Rahman S. M. Saad Us Siraj Mahbubul Karim Abdul Muktadir Arpan Maheshwari Md Azizul Kabir Zebun Nahar Mohammad Mainul Ahasan |
| author_facet | Imran Khan K. M. Taufiqur Rahman S. M. Saad Us Siraj Mahbubul Karim Abdul Muktadir Arpan Maheshwari Md Azizul Kabir Zebun Nahar Mohammad Mainul Ahasan |
| author_sort | Imran Khan |
| collection | DOAJ |
| description | Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd., Bangladesh. A quantitative rocket immunoelectrophoresis assay was employed to determine the polysaccharide contents of each serogroup. The calculated distribution coefficient values of serogroups A, C, W, and Y were found to be 0.26±0.16, 0.21±0.11, 0.21±0.11, and 0.14±0.12, respectively, and met the requirements of British Pharmacopeia. The method was proved to be robust for determining the distribution coefficient values which is an obligatory requirement for vaccine lot release. |
| format | Article |
| id | doaj-art-a0adafc5dad1450a859ef99e9792f17b |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-a0adafc5dad1450a859ef99e9792f17b2025-02-03T01:02:11ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792016-01-01201610.1155/2016/94040689404068A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide VaccineImran Khan0K. M. Taufiqur Rahman1S. M. Saad Us Siraj2Mahbubul Karim3Abdul Muktadir4Arpan Maheshwari5Md Azizul Kabir6Zebun Nahar7Mohammad Mainul Ahasan8Incepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshIncepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshIncepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshIncepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshIncepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshIncepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshIncepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshIncepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshIncepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341, BangladeshMolecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd., Bangladesh. A quantitative rocket immunoelectrophoresis assay was employed to determine the polysaccharide contents of each serogroup. The calculated distribution coefficient values of serogroups A, C, W, and Y were found to be 0.26±0.16, 0.21±0.11, 0.21±0.11, and 0.14±0.12, respectively, and met the requirements of British Pharmacopeia. The method was proved to be robust for determining the distribution coefficient values which is an obligatory requirement for vaccine lot release.http://dx.doi.org/10.1155/2016/9404068 |
| spellingShingle | Imran Khan K. M. Taufiqur Rahman S. M. Saad Us Siraj Mahbubul Karim Abdul Muktadir Arpan Maheshwari Md Azizul Kabir Zebun Nahar Mohammad Mainul Ahasan A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine International Journal of Analytical Chemistry |
| title | A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine |
| title_full | A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine |
| title_fullStr | A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine |
| title_full_unstemmed | A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine |
| title_short | A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine |
| title_sort | high throughput size exclusion chromatography method to determine the molecular size distribution of meningococcal polysaccharide vaccine |
| url | http://dx.doi.org/10.1155/2016/9404068 |
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