Citronellol inhibits the activity of AtSRT1 to increase IAA content and signal transduction, promoting the growth of A. thaliana
Abstract Histone acetylation is a key epigenetic modification involved in plant development. Although histone deacetylase inhibitors (HDACi) are commonly studied in human diseases, their role in regulating histone deacetylation in plants remains unclear. This study explores the function of Citronell...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-02-01
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| Series: | Chemical and Biological Technologies in Agriculture |
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| Online Access: | https://doi.org/10.1186/s40538-025-00737-7 |
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| author | Jin-Rui Wen Hong-Yan Nie Hong-Xin Liao Fu-Rong Xu Xiao-Yun Liu Xian Dong |
| author_facet | Jin-Rui Wen Hong-Yan Nie Hong-Xin Liao Fu-Rong Xu Xiao-Yun Liu Xian Dong |
| author_sort | Jin-Rui Wen |
| collection | DOAJ |
| description | Abstract Histone acetylation is a key epigenetic modification involved in plant development. Although histone deacetylase inhibitors (HDACi) are commonly studied in human diseases, their role in regulating histone deacetylation in plants remains unclear. This study explores the function of Citronellol, a volatile small molecule, as a plant-derived HDACi using Arabidopsis thaliana (L.) Heynh (A. thaliana) as a model. Citronellol at concentrations of 3 and 6 mM enhanced both root development and aboveground growth. Enzyme activity assays, molecular docking, and molecular dynamics simulations showed that Citronellol binds to specific residues (PHE:64, ARG:65, MET:1, and ILE:214) of the histone deacetylase AtSRT1 in Arabidopsis, inhibiting its activity and elevating H3K9ac levels. Integrated RNA-seq and ChIP-seq analyses revealed that Citronellol increased the expression of genes linked to growth and development, including ATCTH, CPL3, IBR5, TCP4, and KUA1, through enhanced histone acetylation and activation of plant hormone signaling pathways. These findings provide new insights into the epigenetic regulation of plant growth by Citronellol, identifying it as a novel HDACi. Citronellol could serve as an effective plant growth regulator, offering valuable applications for agricultural development. Graphical Abstract |
| format | Article |
| id | doaj-art-a0a911523b9c488cb9b34d135a89cb0a |
| institution | DOAJ |
| issn | 2196-5641 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj-art-a0a911523b9c488cb9b34d135a89cb0a2025-08-20T02:48:30ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412025-02-0112111710.1186/s40538-025-00737-7Citronellol inhibits the activity of AtSRT1 to increase IAA content and signal transduction, promoting the growth of A. thalianaJin-Rui Wen0Hong-Yan Nie1Hong-Xin Liao2Fu-Rong Xu3Xiao-Yun Liu4Xian Dong5School of Chinese Materia Medica, Yunnan University of Chinese MedicineSchool of Chinese Materia Medica, Yunnan University of Chinese MedicineSchool of Chinese Materia Medica, Yunnan University of Chinese MedicineSchool of Chinese Materia Medica, Yunnan University of Chinese MedicineCollege of Life Sciences, Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Jianghan UniversitySchool of Chinese Materia Medica, Yunnan University of Chinese MedicineAbstract Histone acetylation is a key epigenetic modification involved in plant development. Although histone deacetylase inhibitors (HDACi) are commonly studied in human diseases, their role in regulating histone deacetylation in plants remains unclear. This study explores the function of Citronellol, a volatile small molecule, as a plant-derived HDACi using Arabidopsis thaliana (L.) Heynh (A. thaliana) as a model. Citronellol at concentrations of 3 and 6 mM enhanced both root development and aboveground growth. Enzyme activity assays, molecular docking, and molecular dynamics simulations showed that Citronellol binds to specific residues (PHE:64, ARG:65, MET:1, and ILE:214) of the histone deacetylase AtSRT1 in Arabidopsis, inhibiting its activity and elevating H3K9ac levels. Integrated RNA-seq and ChIP-seq analyses revealed that Citronellol increased the expression of genes linked to growth and development, including ATCTH, CPL3, IBR5, TCP4, and KUA1, through enhanced histone acetylation and activation of plant hormone signaling pathways. These findings provide new insights into the epigenetic regulation of plant growth by Citronellol, identifying it as a novel HDACi. Citronellol could serve as an effective plant growth regulator, offering valuable applications for agricultural development. Graphical Abstracthttps://doi.org/10.1186/s40538-025-00737-7Essential oilHDACiHistone deacetylasePlant hormonesPromoting mechanisms |
| spellingShingle | Jin-Rui Wen Hong-Yan Nie Hong-Xin Liao Fu-Rong Xu Xiao-Yun Liu Xian Dong Citronellol inhibits the activity of AtSRT1 to increase IAA content and signal transduction, promoting the growth of A. thaliana Chemical and Biological Technologies in Agriculture Essential oil HDACi Histone deacetylase Plant hormones Promoting mechanisms |
| title | Citronellol inhibits the activity of AtSRT1 to increase IAA content and signal transduction, promoting the growth of A. thaliana |
| title_full | Citronellol inhibits the activity of AtSRT1 to increase IAA content and signal transduction, promoting the growth of A. thaliana |
| title_fullStr | Citronellol inhibits the activity of AtSRT1 to increase IAA content and signal transduction, promoting the growth of A. thaliana |
| title_full_unstemmed | Citronellol inhibits the activity of AtSRT1 to increase IAA content and signal transduction, promoting the growth of A. thaliana |
| title_short | Citronellol inhibits the activity of AtSRT1 to increase IAA content and signal transduction, promoting the growth of A. thaliana |
| title_sort | citronellol inhibits the activity of atsrt1 to increase iaa content and signal transduction promoting the growth of a thaliana |
| topic | Essential oil HDACi Histone deacetylase Plant hormones Promoting mechanisms |
| url | https://doi.org/10.1186/s40538-025-00737-7 |
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