Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina.
ABSTRACT: Objective: To characterise four blaNDM-5-harbouring plasmids recovered in Enterobacterales isolated in Argentina. Methods: DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and...
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004363 |
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| author | Francisco González-Espinosa Vincenzo Di Pilato Luca Calabrese Elisa Costa Agustina Costa Gabriel Gutkind Daniela Cejas Marcela Radice |
| author_facet | Francisco González-Espinosa Vincenzo Di Pilato Luca Calabrese Elisa Costa Agustina Costa Gabriel Gutkind Daniela Cejas Marcela Radice |
| author_sort | Francisco González-Espinosa |
| collection | DOAJ |
| description | ABSTRACT: Objective: To characterise four blaNDM-5-harbouring plasmids recovered in Enterobacterales isolated in Argentina. Methods: DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. blaNDM-5-harbouring plasmids were compared with similar deposited plasmids using PLSDB. Results: Two plasmids belonged to incompatibility group IncFII where blaNDM-5 was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring blaNDM-5 in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing blaNDM-5 in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying blaCTX−M-15 and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM. Conclusions: This study broadens the limited understanding of blaNDM-5-harbouring plasmids in Latin America. Furthermore, it represents the first description of blaNDM-5 in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM. |
| format | Article |
| id | doaj-art-a0a74ad998b346fca661e1aeec28b914 |
| institution | OA Journals |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-a0a74ad998b346fca661e1aeec28b9142025-08-20T02:35:03ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-013922422610.1016/j.jgar.2024.10.258Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina.Francisco González-Espinosa0Vincenzo Di Pilato1Luca Calabrese2Elisa Costa3Agustina Costa4Gabriel Gutkind5Daniela Cejas6Marcela Radice7Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Ciudad Autónoma de Buenos Aires, Argentina; CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Ciudad Autónoma de Buenos Aires, ArgentinaUniversity of Genoa, Department of Surgical Sciences and Integrated Diagnostics (DISC), Genoa, Italy; IRCCS Ospedale Policlinico San Martino, Microbiology Unit, Genoa, ItalyUniversity of Genoa, Department of Surgical Sciences and Integrated Diagnostics (DISC), Genoa, ItalyUniversity of Genoa, Department of Surgical Sciences and Integrated Diagnostics (DISC), Genoa, ItalyUniversidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Ciudad Autónoma de Buenos Aires, ArgentinaUniversidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Ciudad Autónoma de Buenos Aires, Argentina; CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Ciudad Autónoma de Buenos Aires, ArgentinaUniversidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Ciudad Autónoma de Buenos Aires, Argentina; CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Ciudad Autónoma de Buenos Aires, Argentina; Corresponding author. Mailing address: Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, IBaViM, Ciudad Autónoma de Buenos Aires, Argentina.Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Ciudad Autónoma de Buenos Aires, Argentina; CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Ciudad Autónoma de Buenos Aires, ArgentinaABSTRACT: Objective: To characterise four blaNDM-5-harbouring plasmids recovered in Enterobacterales isolated in Argentina. Methods: DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. blaNDM-5-harbouring plasmids were compared with similar deposited plasmids using PLSDB. Results: Two plasmids belonged to incompatibility group IncFII where blaNDM-5 was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring blaNDM-5 in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing blaNDM-5 in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying blaCTX−M-15 and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM. Conclusions: This study broadens the limited understanding of blaNDM-5-harbouring plasmids in Latin America. Furthermore, it represents the first description of blaNDM-5 in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM.http://www.sciencedirect.com/science/article/pii/S2213716524004363NDM-5IncFIIIncFIB-IncHI1bEnterobacteralesPlasmidsLatin America |
| spellingShingle | Francisco González-Espinosa Vincenzo Di Pilato Luca Calabrese Elisa Costa Agustina Costa Gabriel Gutkind Daniela Cejas Marcela Radice Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina. Journal of Global Antimicrobial Resistance NDM-5 IncFII IncFIB-IncHI1b Enterobacterales Plasmids Latin America |
| title | Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina. |
| title_full | Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina. |
| title_fullStr | Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina. |
| title_full_unstemmed | Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina. |
| title_short | Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina. |
| title_sort | integral genomic description of blandm 5 harbouring plasmids recovered from enterobacterales in argentina |
| topic | NDM-5 IncFII IncFIB-IncHI1b Enterobacterales Plasmids Latin America |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004363 |
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