Exploring the antimicrobial potential of the articaine derivative in oral infections

Objective Postoperative infection is one of the most common complications in dental procedures. During local anesthesia in dental treatments, the risk of postoperative infections increases if the oral mucosa is infected, the anesthesia injection site is inadequately disinfected, or the injection nee...

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Main Authors: Yongzhen Tan, Yihang Hao, Yihang Fu, Shilong Hu, Xi Yang, Yaling Tang, Bowen Ke, Xinhua Liang
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Oral Microbiology
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Online Access:https://www.tandfonline.com/doi/10.1080/20002297.2025.2502455
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author Yongzhen Tan
Yihang Hao
Yihang Fu
Shilong Hu
Xi Yang
Yaling Tang
Bowen Ke
Xinhua Liang
author_facet Yongzhen Tan
Yihang Hao
Yihang Fu
Shilong Hu
Xi Yang
Yaling Tang
Bowen Ke
Xinhua Liang
author_sort Yongzhen Tan
collection DOAJ
description Objective Postoperative infection is one of the most common complications in dental procedures. During local anesthesia in dental treatments, the risk of postoperative infections increases if the oral mucosa is infected, the anesthesia injection site is inadequately disinfected, or the injection needle and anesthetic drugs are contaminated. Thus, developing new oral local anesthetics that offer superior anesthesia, enhanced safety, and antimicrobial properties could greatly enhance their clinical value.Methods The anesthetic effects and antibacterial properties of articaine derivatives were screened using membrane chromatography techniques, animal experiments, and cellular molecular assays. Safety assessments were conducted on the selected target compounds. Additionally, the antibacterial mechanisms of the compounds were investigated through molecular dynamics simulations and cryo-electron microscopy.Results Through the screening of articaine derivatives, a novel local anesthetic, AT-15, was identified, which combines effective anesthetic properties with antibacterial activity. This compound exhibits strong pharmacological activity and high safety. Its antibacterial effect is believed to result from the disruption of bacterial cell membranes and the inhibition of topoisomerase, an enzyme essential for bacterial DNA synthesis.Conclusion AT-15 discovered in this study is a promising candidate for further development in clinical settings.
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publishDate 2025-12-01
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record_format Article
series Journal of Oral Microbiology
spelling doaj-art-a09f48fd2f584209af5e7608d750b7b02025-08-20T01:49:43ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2025.2502455Exploring the antimicrobial potential of the articaine derivative in oral infectionsYongzhen Tan0Yihang Hao1Yihang Fu2Shilong Hu3Xi Yang4Yaling Tang5Bowen Ke6Xinhua Liang7State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Anesthesiology, Laboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Anesthesiology, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, ChinaState Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Anesthesiology, Laboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaObjective Postoperative infection is one of the most common complications in dental procedures. During local anesthesia in dental treatments, the risk of postoperative infections increases if the oral mucosa is infected, the anesthesia injection site is inadequately disinfected, or the injection needle and anesthetic drugs are contaminated. Thus, developing new oral local anesthetics that offer superior anesthesia, enhanced safety, and antimicrobial properties could greatly enhance their clinical value.Methods The anesthetic effects and antibacterial properties of articaine derivatives were screened using membrane chromatography techniques, animal experiments, and cellular molecular assays. Safety assessments were conducted on the selected target compounds. Additionally, the antibacterial mechanisms of the compounds were investigated through molecular dynamics simulations and cryo-electron microscopy.Results Through the screening of articaine derivatives, a novel local anesthetic, AT-15, was identified, which combines effective anesthetic properties with antibacterial activity. This compound exhibits strong pharmacological activity and high safety. Its antibacterial effect is believed to result from the disruption of bacterial cell membranes and the inhibition of topoisomerase, an enzyme essential for bacterial DNA synthesis.Conclusion AT-15 discovered in this study is a promising candidate for further development in clinical settings.https://www.tandfonline.com/doi/10.1080/20002297.2025.2502455Local dental anestheticsarticainecell membrane chromatographyantibacterial activitysafety evaluation
spellingShingle Yongzhen Tan
Yihang Hao
Yihang Fu
Shilong Hu
Xi Yang
Yaling Tang
Bowen Ke
Xinhua Liang
Exploring the antimicrobial potential of the articaine derivative in oral infections
Journal of Oral Microbiology
Local dental anesthetics
articaine
cell membrane chromatography
antibacterial activity
safety evaluation
title Exploring the antimicrobial potential of the articaine derivative in oral infections
title_full Exploring the antimicrobial potential of the articaine derivative in oral infections
title_fullStr Exploring the antimicrobial potential of the articaine derivative in oral infections
title_full_unstemmed Exploring the antimicrobial potential of the articaine derivative in oral infections
title_short Exploring the antimicrobial potential of the articaine derivative in oral infections
title_sort exploring the antimicrobial potential of the articaine derivative in oral infections
topic Local dental anesthetics
articaine
cell membrane chromatography
antibacterial activity
safety evaluation
url https://www.tandfonline.com/doi/10.1080/20002297.2025.2502455
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