Molecular Characterization of Clinical Isolates of Methicillin-Resistant <i>Staphylococcus aureus</i> from Chonburi, Thailand
<i>Staphylococcus aureus</i> (<i>S. aureus</i>), especially methicillin-resistant <i>S. aureus</i> (MRSA), remains a major public health concern. This study reports the antimicrobial resistance profiles and molecular characteristics of 31 <i>S. aureus</i&...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/14/5/406 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850256449543864320 |
|---|---|
| author | Patcharawalai Wassanarungroj Panida Nobthai Sirigade Ruekit Apichai Srijan Prawet Sukhchat Oralak Serichantalergs John M. Crawford Brett E. Swierczewski Sidhartha Chaudhury Paphavee Lertsethtakarn |
| author_facet | Patcharawalai Wassanarungroj Panida Nobthai Sirigade Ruekit Apichai Srijan Prawet Sukhchat Oralak Serichantalergs John M. Crawford Brett E. Swierczewski Sidhartha Chaudhury Paphavee Lertsethtakarn |
| author_sort | Patcharawalai Wassanarungroj |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> (<i>S. aureus</i>), especially methicillin-resistant <i>S. aureus</i> (MRSA), remains a major public health concern. This study reports the antimicrobial resistance profiles and molecular characteristics of 31 <i>S. aureus</i> isolated during 2017–2018 from inpatient and outpatient clinical specimens from Queen Sirikit Naval Hospital (QSH) in Chonburi province, Thailand. All isolates were tested for antimicrobial susceptibility. Staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>) typing, Panton–Valentine leukocidin (<i>pvl</i>) toxin, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and staphylococcal protein A (<i>spa</i>) typing were performed. Twenty-seven isolates were confirmed to be MRSA and exhibited resistance to up to seven antibiotics classes. The main MLST type was SCC<i>mec</i> type II (51.9%) and ST764 (55.6%). Five <i>spa</i> types were identified with t045 (55.6%) as the major type. All 31 <i>S. aureus</i> isolates were grouped into seven types using PFGE with the SCC<i>mec</i>II-ST764-t045 clone being the most prevalent. Overall, our findings reveal that the <i>S. aureus</i> isolates in this study differ from previous reports in Thailand, indicating a potential shift in local strains, highlighting the need for ongoing molecular surveillance of multidrug resistance patterns of MRSA in Southeast Asia. |
| format | Article |
| id | doaj-art-a09855eb43cc45bc816de1163dd545cb |
| institution | OA Journals |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-a09855eb43cc45bc816de1163dd545cb2025-08-20T01:56:38ZengMDPI AGPathogens2076-08172025-04-0114540610.3390/pathogens14050406Molecular Characterization of Clinical Isolates of Methicillin-Resistant <i>Staphylococcus aureus</i> from Chonburi, ThailandPatcharawalai Wassanarungroj0Panida Nobthai1Sirigade Ruekit2Apichai Srijan3Prawet Sukhchat4Oralak Serichantalergs5John M. Crawford6Brett E. Swierczewski7Sidhartha Chaudhury8Paphavee Lertsethtakarn9Department of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), 315/6 Rajvithi Rd., Bangkok 10400, ThailandDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), 315/6 Rajvithi Rd., Bangkok 10400, ThailandDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), 315/6 Rajvithi Rd., Bangkok 10400, ThailandDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), 315/6 Rajvithi Rd., Bangkok 10400, ThailandQueen Sirikit Naval Hospital, Chonburi 20180, ThailandDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), 315/6 Rajvithi Rd., Bangkok 10400, ThailandUS Army Research Institute of Chemical Defense, Gunpowder, MA 21010, USAUS Army Medical Research Institute of Infectious Diseases, Frederick, MA 21702, USADepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), 315/6 Rajvithi Rd., Bangkok 10400, ThailandDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), 315/6 Rajvithi Rd., Bangkok 10400, Thailand<i>Staphylococcus aureus</i> (<i>S. aureus</i>), especially methicillin-resistant <i>S. aureus</i> (MRSA), remains a major public health concern. This study reports the antimicrobial resistance profiles and molecular characteristics of 31 <i>S. aureus</i> isolated during 2017–2018 from inpatient and outpatient clinical specimens from Queen Sirikit Naval Hospital (QSH) in Chonburi province, Thailand. All isolates were tested for antimicrobial susceptibility. Staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>) typing, Panton–Valentine leukocidin (<i>pvl</i>) toxin, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and staphylococcal protein A (<i>spa</i>) typing were performed. Twenty-seven isolates were confirmed to be MRSA and exhibited resistance to up to seven antibiotics classes. The main MLST type was SCC<i>mec</i> type II (51.9%) and ST764 (55.6%). Five <i>spa</i> types were identified with t045 (55.6%) as the major type. All 31 <i>S. aureus</i> isolates were grouped into seven types using PFGE with the SCC<i>mec</i>II-ST764-t045 clone being the most prevalent. Overall, our findings reveal that the <i>S. aureus</i> isolates in this study differ from previous reports in Thailand, indicating a potential shift in local strains, highlighting the need for ongoing molecular surveillance of multidrug resistance patterns of MRSA in Southeast Asia.https://www.mdpi.com/2076-0817/14/5/406MRSASCC<i>mec</i>MLSTPFGE<i>spa</i>PVL |
| spellingShingle | Patcharawalai Wassanarungroj Panida Nobthai Sirigade Ruekit Apichai Srijan Prawet Sukhchat Oralak Serichantalergs John M. Crawford Brett E. Swierczewski Sidhartha Chaudhury Paphavee Lertsethtakarn Molecular Characterization of Clinical Isolates of Methicillin-Resistant <i>Staphylococcus aureus</i> from Chonburi, Thailand Pathogens MRSA SCC<i>mec</i> MLST PFGE <i>spa</i> PVL |
| title | Molecular Characterization of Clinical Isolates of Methicillin-Resistant <i>Staphylococcus aureus</i> from Chonburi, Thailand |
| title_full | Molecular Characterization of Clinical Isolates of Methicillin-Resistant <i>Staphylococcus aureus</i> from Chonburi, Thailand |
| title_fullStr | Molecular Characterization of Clinical Isolates of Methicillin-Resistant <i>Staphylococcus aureus</i> from Chonburi, Thailand |
| title_full_unstemmed | Molecular Characterization of Clinical Isolates of Methicillin-Resistant <i>Staphylococcus aureus</i> from Chonburi, Thailand |
| title_short | Molecular Characterization of Clinical Isolates of Methicillin-Resistant <i>Staphylococcus aureus</i> from Chonburi, Thailand |
| title_sort | molecular characterization of clinical isolates of methicillin resistant i staphylococcus aureus i from chonburi thailand |
| topic | MRSA SCC<i>mec</i> MLST PFGE <i>spa</i> PVL |
| url | https://www.mdpi.com/2076-0817/14/5/406 |
| work_keys_str_mv | AT patcharawalaiwassanarungroj molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT panidanobthai molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT sirigaderuekit molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT apichaisrijan molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT prawetsukhchat molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT oralakserichantalergs molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT johnmcrawford molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT bretteswierczewski molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT sidharthachaudhury molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand AT paphaveelertsethtakarn molecularcharacterizationofclinicalisolatesofmethicillinresistantistaphylococcusaureusifromchonburithailand |