Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camels
Our study aimed to explore the genetic variation in the Toll-like receptor 4 (TLR4) gene and establish its association with somatic cell score (SCS) and milk production traits in four Indian camel breeds namely Bikaneri, Kachchhi, Jaisalmeri and Mewari. TLR4 gene fragment of 573 bp spanning 5’ UTR,...
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2024.2331642 |
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| author | Seema Bishnoi Basanti Jyotsana Virendra Kumar Ved Prakash Rakesh Ranjan Shirish Dadarao Narnaware Urmila Pannu |
| author_facet | Seema Bishnoi Basanti Jyotsana Virendra Kumar Ved Prakash Rakesh Ranjan Shirish Dadarao Narnaware Urmila Pannu |
| author_sort | Seema Bishnoi |
| collection | DOAJ |
| description | Our study aimed to explore the genetic variation in the Toll-like receptor 4 (TLR4) gene and establish its association with somatic cell score (SCS) and milk production traits in four Indian camel breeds namely Bikaneri, Kachchhi, Jaisalmeri and Mewari. TLR4 gene fragment of 573 bp spanning 5’ UTR, exon-1 and partial intron-1 region was amplified and genotyped using the PCR-sequence based typing method. Only one SNP located at position C472T was identified. Genotyping revealed two alleles (C and T) and three genotypes: CC, CT and TT. The genotype frequencies for CC, CT and TT were 0.116, 0.326 and 0.558 and allele frequencies for C and T alleles were 0.279 and 0.721, respectively. Association study inferred that the effect of genotype on SCS, lactation yield (LY) and peak yield (PY) was non-significant however heterozygote (CT) genotypes recorded lower SCS and higher LY and PY. It can be concluded that the TLR4 gene possesses limited genetic variation, depicting polymorphism at a single locus in Indian camel breeds with a predominance of the TT genotype. The association study indicated that heterozygote animals possess better udder health and production performance, the statistical significance of which needs to be established using a large data set. |
| format | Article |
| id | doaj-art-a0984017c57d4bae97fc9e2297807e1d |
| institution | DOAJ |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-a0984017c57d4bae97fc9e2297807e1d2025-08-20T02:50:22ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2331642Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camelsSeema Bishnoi0Basanti Jyotsana1Virendra Kumar2Ved Prakash3Rakesh Ranjan4Shirish Dadarao Narnaware5Urmila Pannu6CVAS, Rajasthan University of Veterinary & Animal Sciences, Bikaner, Rajasthan, IndiaICAR – National Research Centre on Camel, Bikaner, Rajasthan, IndiaCVAS, Rajasthan University of Veterinary & Animal Sciences, Bikaner, Rajasthan, IndiaICAR – National Research Centre on Camel, Bikaner, Rajasthan, IndiaICAR – National Research Centre on Camel, Bikaner, Rajasthan, IndiaICAR – Central Coastal Agricultural Research Institute, Ela, Old Goa, Goa, IndiaCVAS, Rajasthan University of Veterinary & Animal Sciences, Bikaner, Rajasthan, IndiaOur study aimed to explore the genetic variation in the Toll-like receptor 4 (TLR4) gene and establish its association with somatic cell score (SCS) and milk production traits in four Indian camel breeds namely Bikaneri, Kachchhi, Jaisalmeri and Mewari. TLR4 gene fragment of 573 bp spanning 5’ UTR, exon-1 and partial intron-1 region was amplified and genotyped using the PCR-sequence based typing method. Only one SNP located at position C472T was identified. Genotyping revealed two alleles (C and T) and three genotypes: CC, CT and TT. The genotype frequencies for CC, CT and TT were 0.116, 0.326 and 0.558 and allele frequencies for C and T alleles were 0.279 and 0.721, respectively. Association study inferred that the effect of genotype on SCS, lactation yield (LY) and peak yield (PY) was non-significant however heterozygote (CT) genotypes recorded lower SCS and higher LY and PY. It can be concluded that the TLR4 gene possesses limited genetic variation, depicting polymorphism at a single locus in Indian camel breeds with a predominance of the TT genotype. The association study indicated that heterozygote animals possess better udder health and production performance, the statistical significance of which needs to be established using a large data set.https://www.tandfonline.com/doi/10.1080/10495398.2024.2331642Associationcamelgenepolymorphismsomatic cell scoreTLR4 |
| spellingShingle | Seema Bishnoi Basanti Jyotsana Virendra Kumar Ved Prakash Rakesh Ranjan Shirish Dadarao Narnaware Urmila Pannu Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camels Animal Biotechnology Association camel gene polymorphism somatic cell score TLR4 |
| title | Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camels |
| title_full | Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camels |
| title_fullStr | Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camels |
| title_full_unstemmed | Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camels |
| title_short | Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camels |
| title_sort | toll like receptor 4 tlr4 gene polymorphism and its association with somatic cell score and milk production traits in indian dromedary camels |
| topic | Association camel gene polymorphism somatic cell score TLR4 |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2024.2331642 |
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