Effects of Phytosterols on Growth Performance, Serum Indexes, and Fecal Microbiota in Finishing Pigs

Phytosterols (PSs) are widely present in plants, particularly abundant in plant oils and seeds. PSs are reported to have various biological activities, such as lowering cholesterol, alongside antioxidant and antibacterial activities. This research examined the effects of PSs in finishing pigs, inclu...

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Main Authors: Renjie Xie, Zhenxing Guo, Haiqing Gan, Dexing Hou, Guang Chen, Chao Deng, Hongkun Li, Jiajie Ouyang, Qiyu Tian, Xingguo Huang
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/9/1188
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author Renjie Xie
Zhenxing Guo
Haiqing Gan
Dexing Hou
Guang Chen
Chao Deng
Hongkun Li
Jiajie Ouyang
Qiyu Tian
Xingguo Huang
author_facet Renjie Xie
Zhenxing Guo
Haiqing Gan
Dexing Hou
Guang Chen
Chao Deng
Hongkun Li
Jiajie Ouyang
Qiyu Tian
Xingguo Huang
author_sort Renjie Xie
collection DOAJ
description Phytosterols (PSs) are widely present in plants, particularly abundant in plant oils and seeds. PSs are reported to have various biological activities, such as lowering cholesterol, alongside antioxidant and antibacterial activities. This research examined the effects of PSs in finishing pigs, including growth performance, serum biochemistry, and fecal bacteria. Two treatment groups (each treatment group consisted of five biological replicates, and each replicate comprised five pigs housed communally) were randomly assigned to the fifty finishing pigs (equally divided by sex) of “Duroc × Landrace × Yorkshire” three-way cross with 79.76 ± 1.29 (kg) body weight. The control group (CON) was given basic food, while the experimental group was given basic food containing 300 mg PS/kg (PS). Dietary PS supplementation markedly raised the levels of average daily feed intake (ADFI) and apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and gross energy (GE) in comparison to the CON (<i>p</i> < 0.05). Additionally, PSs also significantly boosted the concentrations of high-density lipoprotein cholesterol (HDL-C), total protein (TP), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), immunoglobulin G (IgG), motilin (MTL), and glucagon-like peptide-1 (GLP-1) (<i>p</i> < 0.05), and lowered the lactate dehydrogenase (LDH) level (<i>p</i> < 0.05). Both at the phyla and genus levels, the relative abundance of Firmicutes and <i>Streptococcus</i> increased significantly, and the relative abundance of <i>Acinetobacter</i> decreased significantly when adding phytosterols (<i>p</i> < 0.05). Overall, phytosterols dietary supplementation promotes immunity and antioxidant capacity in finishing pigs and boosts growth performance by improving nutrient digestibility.
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series Animals
spelling doaj-art-a08e2a4c3bc5475fa6e35b6cc91d2f062025-08-20T01:49:24ZengMDPI AGAnimals2076-26152025-04-01159118810.3390/ani15091188Effects of Phytosterols on Growth Performance, Serum Indexes, and Fecal Microbiota in Finishing PigsRenjie Xie0Zhenxing Guo1Haiqing Gan2Dexing Hou3Guang Chen4Chao Deng5Hongkun Li6Jiajie Ouyang7Qiyu Tian8Xingguo Huang9College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaGraduate School of Agriculture, Forestry and Fisheries, Kagoshima University, Kagoshima 890-0065, JapanCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaHunan Biological and Electromechanical Polytechnic, Changsha 210127, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaPhytosterols (PSs) are widely present in plants, particularly abundant in plant oils and seeds. PSs are reported to have various biological activities, such as lowering cholesterol, alongside antioxidant and antibacterial activities. This research examined the effects of PSs in finishing pigs, including growth performance, serum biochemistry, and fecal bacteria. Two treatment groups (each treatment group consisted of five biological replicates, and each replicate comprised five pigs housed communally) were randomly assigned to the fifty finishing pigs (equally divided by sex) of “Duroc × Landrace × Yorkshire” three-way cross with 79.76 ± 1.29 (kg) body weight. The control group (CON) was given basic food, while the experimental group was given basic food containing 300 mg PS/kg (PS). Dietary PS supplementation markedly raised the levels of average daily feed intake (ADFI) and apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and gross energy (GE) in comparison to the CON (<i>p</i> < 0.05). Additionally, PSs also significantly boosted the concentrations of high-density lipoprotein cholesterol (HDL-C), total protein (TP), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), immunoglobulin G (IgG), motilin (MTL), and glucagon-like peptide-1 (GLP-1) (<i>p</i> < 0.05), and lowered the lactate dehydrogenase (LDH) level (<i>p</i> < 0.05). Both at the phyla and genus levels, the relative abundance of Firmicutes and <i>Streptococcus</i> increased significantly, and the relative abundance of <i>Acinetobacter</i> decreased significantly when adding phytosterols (<i>p</i> < 0.05). Overall, phytosterols dietary supplementation promotes immunity and antioxidant capacity in finishing pigs and boosts growth performance by improving nutrient digestibility.https://www.mdpi.com/2076-2615/15/9/1188phytosterolsfattening pigsapparent digestibilityserum parametersantioxidant capacityfecal flora
spellingShingle Renjie Xie
Zhenxing Guo
Haiqing Gan
Dexing Hou
Guang Chen
Chao Deng
Hongkun Li
Jiajie Ouyang
Qiyu Tian
Xingguo Huang
Effects of Phytosterols on Growth Performance, Serum Indexes, and Fecal Microbiota in Finishing Pigs
Animals
phytosterols
fattening pigs
apparent digestibility
serum parameters
antioxidant capacity
fecal flora
title Effects of Phytosterols on Growth Performance, Serum Indexes, and Fecal Microbiota in Finishing Pigs
title_full Effects of Phytosterols on Growth Performance, Serum Indexes, and Fecal Microbiota in Finishing Pigs
title_fullStr Effects of Phytosterols on Growth Performance, Serum Indexes, and Fecal Microbiota in Finishing Pigs
title_full_unstemmed Effects of Phytosterols on Growth Performance, Serum Indexes, and Fecal Microbiota in Finishing Pigs
title_short Effects of Phytosterols on Growth Performance, Serum Indexes, and Fecal Microbiota in Finishing Pigs
title_sort effects of phytosterols on growth performance serum indexes and fecal microbiota in finishing pigs
topic phytosterols
fattening pigs
apparent digestibility
serum parameters
antioxidant capacity
fecal flora
url https://www.mdpi.com/2076-2615/15/9/1188
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