Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice

Background. The spread of drug resistance is a significant issue, particularly in endemic countries with limited resources. The aim of this study was to evaluate antimalarial and antioxidant activity of B. micrantha in order to justify its use in traditional medicine. Methods. Evaluation of the in v...

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Main Authors: Tako Djimefo Alex Kevin, Yamssi Cedric, Noumedem Anangmo Christelle Nadia, Tientcheu Noutong Jemimah Sandra, Mounvera Abdel Azizi, Ngouyamsa Nsapkain Aboubakar Sidiki, Gamago Nkadeu Guy-Armand, Mbohou Nchetnkou Christian, Essangui Same Estelle Géraldine, Tankoua-Tchounda Roméo, Vincent Khan Payne, Lehman Léopold Gustave
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Journal of Parasitology Research
Online Access:http://dx.doi.org/10.1155/2024/8821019
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author Tako Djimefo Alex Kevin
Yamssi Cedric
Noumedem Anangmo Christelle Nadia
Tientcheu Noutong Jemimah Sandra
Mounvera Abdel Azizi
Ngouyamsa Nsapkain Aboubakar Sidiki
Gamago Nkadeu Guy-Armand
Mbohou Nchetnkou Christian
Essangui Same Estelle Géraldine
Tankoua-Tchounda Roméo
Vincent Khan Payne
Lehman Léopold Gustave
author_facet Tako Djimefo Alex Kevin
Yamssi Cedric
Noumedem Anangmo Christelle Nadia
Tientcheu Noutong Jemimah Sandra
Mounvera Abdel Azizi
Ngouyamsa Nsapkain Aboubakar Sidiki
Gamago Nkadeu Guy-Armand
Mbohou Nchetnkou Christian
Essangui Same Estelle Géraldine
Tankoua-Tchounda Roméo
Vincent Khan Payne
Lehman Léopold Gustave
author_sort Tako Djimefo Alex Kevin
collection DOAJ
description Background. The spread of drug resistance is a significant issue, particularly in endemic countries with limited resources. The aim of this study was to evaluate antimalarial and antioxidant activity of B. micrantha in order to justify its use in traditional medicine. Methods. Evaluation of the in vivo antimalarial activity of B. micrantha was carried out according to the model of the suppressive and curative test of Peters’ over 4 days in infected Swiss albino mice. Antioxidant parameters and stress were measured after intraperitoneal administration of 1×107 infected red blood cells. Results. At doses of 150 mg/kg, 300 mg/kg, and 600 mg/kg, administration of B. micrantha substantially produced suppression of P. berghei infection by 67.75%, 73.46%, and 78.99%, respectively, while 84.64% of the untreated group (1% DMSO) had suppression from chloroquine. The curative test significantly decreased the levels of parasitaemia and death in the treated groups. Furthermore, after B. micrantha extract was given to infected mice, a noteworthy increase in total protein, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) was observed. On the other hand, hepatic catalase (CAT) and superoxide dismutase (SOD) productions were considerably greater than that of the healthy control. Mice had considerably lower levels of nonenzymatic antioxidant markers such as glutathione, NO, and MDA showing that the liver was protected. Conclusion. The infected groups responded favorably to the ethanol extract of B. micrantha. This result justifies investigation for its use in Cameroon.
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spelling doaj-art-a0840280755e4f25b2e40ebeec1b287d2025-08-20T03:38:09ZengWileyJournal of Parasitology Research2090-00312024-01-01202410.1155/2024/8821019Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected MiceTako Djimefo Alex Kevin0Yamssi Cedric1Noumedem Anangmo Christelle Nadia2Tientcheu Noutong Jemimah Sandra3Mounvera Abdel Azizi4Ngouyamsa Nsapkain Aboubakar Sidiki5Gamago Nkadeu Guy-Armand6Mbohou Nchetnkou Christian7Essangui Same Estelle Géraldine8Tankoua-Tchounda Roméo9Vincent Khan Payne10Lehman Léopold Gustave11Department of Animal OrganismsLaboratory of Tropical and Emerging Infectious DiseasesLaboratory of Tropical and Emerging Infectious DiseasesLaboratory of Tropical and Emerging Infectious DiseasesLaboratory of Tropical and Emerging Infectious DiseasesLaboratory of Tropical and Emerging Infectious DiseasesLaboratory of Tropical and Emerging Infectious DiseasesDepartment of Animal OrganismsDepartment of Biological SciencesDepartment of Animal OrganismsLaboratory of Tropical and Emerging Infectious DiseasesDepartment of Animal OrganismsBackground. The spread of drug resistance is a significant issue, particularly in endemic countries with limited resources. The aim of this study was to evaluate antimalarial and antioxidant activity of B. micrantha in order to justify its use in traditional medicine. Methods. Evaluation of the in vivo antimalarial activity of B. micrantha was carried out according to the model of the suppressive and curative test of Peters’ over 4 days in infected Swiss albino mice. Antioxidant parameters and stress were measured after intraperitoneal administration of 1×107 infected red blood cells. Results. At doses of 150 mg/kg, 300 mg/kg, and 600 mg/kg, administration of B. micrantha substantially produced suppression of P. berghei infection by 67.75%, 73.46%, and 78.99%, respectively, while 84.64% of the untreated group (1% DMSO) had suppression from chloroquine. The curative test significantly decreased the levels of parasitaemia and death in the treated groups. Furthermore, after B. micrantha extract was given to infected mice, a noteworthy increase in total protein, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) was observed. On the other hand, hepatic catalase (CAT) and superoxide dismutase (SOD) productions were considerably greater than that of the healthy control. Mice had considerably lower levels of nonenzymatic antioxidant markers such as glutathione, NO, and MDA showing that the liver was protected. Conclusion. The infected groups responded favorably to the ethanol extract of B. micrantha. This result justifies investigation for its use in Cameroon.http://dx.doi.org/10.1155/2024/8821019
spellingShingle Tako Djimefo Alex Kevin
Yamssi Cedric
Noumedem Anangmo Christelle Nadia
Tientcheu Noutong Jemimah Sandra
Mounvera Abdel Azizi
Ngouyamsa Nsapkain Aboubakar Sidiki
Gamago Nkadeu Guy-Armand
Mbohou Nchetnkou Christian
Essangui Same Estelle Géraldine
Tankoua-Tchounda Roméo
Vincent Khan Payne
Lehman Léopold Gustave
Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice
Journal of Parasitology Research
title Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice
title_full Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice
title_fullStr Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice
title_full_unstemmed Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice
title_short Antimalarial Efficacy of Ethanol Extract of Bridelia micrantha Stem Bark against Plasmodium berghei-Infected Mice
title_sort antimalarial efficacy of ethanol extract of bridelia micrantha stem bark against plasmodium berghei infected mice
url http://dx.doi.org/10.1155/2024/8821019
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