Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in <i>Salmonella</i> Enteritidis
Two-component systems (TCS) of <i>Salmonella enterica</i> serovar Enteritidis are composed of a histidine kinase and a response regulator (RR) and represent a critical mechanism by which bacteria develop resistance to environmental stress. Here, we characterized the functions of RRs in T...
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2024-11-01
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| Series: | Foods |
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| author | Mengjun Hu Zhuoan Zhou Chenqi Liu Zeqiang Zhan Yan Cui Shoukui He Xianming Shi |
| author_facet | Mengjun Hu Zhuoan Zhou Chenqi Liu Zeqiang Zhan Yan Cui Shoukui He Xianming Shi |
| author_sort | Mengjun Hu |
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| description | Two-component systems (TCS) of <i>Salmonella enterica</i> serovar Enteritidis are composed of a histidine kinase and a response regulator (RR) and represent a critical mechanism by which bacteria develop resistance to environmental stress. Here, we characterized the functions of RRs in TCS in the formation of stress tolerance, motility and biofilm using twenty-six <i>S</i>. Enteritidis RR-encoding gene deletion mutants. The viability results unraveled their essential roles in resistance to elevated temperature (GlrR), pH alterations (GlrR, TctD, YedW, ArcA and YehT), high salt (PhoB, BaeR, CpxR, PhoP, UvrY and TctD), oxidative stress (PhoB, YedW, BaeR, ArcA, PhoP, UvrY, PgtA and QseB) and motility (ArcA, GlnG, PgtA, PhoB, UhpA, OmpR, UvrY and QseB) of <i>S</i>. Enteritidis. The results of the crystal violet staining, microscopy observation and Congo red binding assays demonstrated that the absence of ArcA, GlnG, PhoP, OmpR, ZraR or SsrB in <i>S</i>. Enteritidis led to a reduction in biofilms and an impairment in red/dry/rough macrocolony formation, whereas the absence of UvrY exhibited an increase in biofilms and formed a brown/smooth/sticky macrocolony. The results indicated the regulatory effects of these RRs on the production of biofilm matrix, curli fimbriae and cellulose. Our findings yielded insights into the role of TCSs, making them a promising target for combating <i>S</i>. Enteritidis. |
| format | Article |
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| issn | 2304-8158 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-a0780a4f4cc542b08dad4d607eb2962f2025-08-20T01:53:48ZengMDPI AGFoods2304-81582024-11-011322370910.3390/foods13223709Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in <i>Salmonella</i> EnteritidisMengjun Hu0Zhuoan Zhou1Chenqi Liu2Zeqiang Zhan3Yan Cui4Shoukui He5Xianming Shi6MOST-USDA Joint Research Center for Food Safety, State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaTwo-component systems (TCS) of <i>Salmonella enterica</i> serovar Enteritidis are composed of a histidine kinase and a response regulator (RR) and represent a critical mechanism by which bacteria develop resistance to environmental stress. Here, we characterized the functions of RRs in TCS in the formation of stress tolerance, motility and biofilm using twenty-six <i>S</i>. Enteritidis RR-encoding gene deletion mutants. The viability results unraveled their essential roles in resistance to elevated temperature (GlrR), pH alterations (GlrR, TctD, YedW, ArcA and YehT), high salt (PhoB, BaeR, CpxR, PhoP, UvrY and TctD), oxidative stress (PhoB, YedW, BaeR, ArcA, PhoP, UvrY, PgtA and QseB) and motility (ArcA, GlnG, PgtA, PhoB, UhpA, OmpR, UvrY and QseB) of <i>S</i>. Enteritidis. The results of the crystal violet staining, microscopy observation and Congo red binding assays demonstrated that the absence of ArcA, GlnG, PhoP, OmpR, ZraR or SsrB in <i>S</i>. Enteritidis led to a reduction in biofilms and an impairment in red/dry/rough macrocolony formation, whereas the absence of UvrY exhibited an increase in biofilms and formed a brown/smooth/sticky macrocolony. The results indicated the regulatory effects of these RRs on the production of biofilm matrix, curli fimbriae and cellulose. Our findings yielded insights into the role of TCSs, making them a promising target for combating <i>S</i>. Enteritidis.https://www.mdpi.com/2304-8158/13/22/3709<i>S</i>. EnteritidisTCSresponse regulatorenvironmental stressbiofilm matrix |
| spellingShingle | Mengjun Hu Zhuoan Zhou Chenqi Liu Zeqiang Zhan Yan Cui Shoukui He Xianming Shi Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in <i>Salmonella</i> Enteritidis Foods <i>S</i>. Enteritidis TCS response regulator environmental stress biofilm matrix |
| title | Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in <i>Salmonella</i> Enteritidis |
| title_full | Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in <i>Salmonella</i> Enteritidis |
| title_fullStr | Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in <i>Salmonella</i> Enteritidis |
| title_full_unstemmed | Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in <i>Salmonella</i> Enteritidis |
| title_short | Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm in <i>Salmonella</i> Enteritidis |
| title_sort | roles of response regulators in the two component system in the formation of stress tolerance motility and biofilm in i salmonella i enteritidis |
| topic | <i>S</i>. Enteritidis TCS response regulator environmental stress biofilm matrix |
| url | https://www.mdpi.com/2304-8158/13/22/3709 |
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