New Chaetoglobosins with Fungicidal Activity from <i>Chaetomium</i> sp. UJN-EF006 Endophytic in <i>Vaccinium bracteatum</i>
Nine chaetoglobosins (<b>1</b>–<b>9</b>) including five previously undescribed ones (<b>1</b>–<b>5</b>) were obtained from the culture broth of an endophytic fungus (<i>Chaetomium</i> sp. UJN-EF006) isolated from the leaves of <i>Vacc...
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2025-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/11/7/511 |
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| author | Luo-Jing Wang Zong-Yan Ma Xin-Ling Wang Kai-Le Wang Tong Zhang Rui-Ying Han Jun-Jiang Li Jie Bao Yin-Yin Wang Hua Zhang |
| author_facet | Luo-Jing Wang Zong-Yan Ma Xin-Ling Wang Kai-Le Wang Tong Zhang Rui-Ying Han Jun-Jiang Li Jie Bao Yin-Yin Wang Hua Zhang |
| author_sort | Luo-Jing Wang |
| collection | DOAJ |
| description | Nine chaetoglobosins (<b>1</b>–<b>9</b>) including five previously undescribed ones (<b>1</b>–<b>5</b>) were obtained from the culture broth of an endophytic fungus (<i>Chaetomium</i> sp. UJN-EF006) isolated from the leaves of <i>Vaccinium bracteatum</i>. The structures of these fungal metabolites were elucidated by spectroscopic methods including mass spectroscopy, nuclear magnetic resonance, single crystal X-ray crystallography, and electronic circular dichroism. To accelerate the development of novel fungicides, all of the isolated chaetoglobosins were evaluated for their antifungal activity against two crop pathogens, <i>Botrytis cinerea</i> and <i>Sclerotinia sclerotiorum</i>. The assay results revealed that chaetoglobosins <b>2</b>, <b>6</b>, <b>7</b>, and <b>9</b> possessed a significant fungicidal effect against <i>B. cinerea</i>, with EC<sub>50</sub> values all below 10 μg/mL. Particularly, the most potent compound, <b>7</b>, was 175- and 96-fold as active as the commercially available fungicides carbendazim (EC<sub>50</sub> 70.11 μg/mL) and azoxystrobin (EC<sub>50</sub> 39.02 μg/mL), respectively. A further observation under scanning electron microscope indicated that compound <b>2</b> could markedly impair the fungal hyphae of <i>B. cinerea</i>. The study demonstrates that the chaetoglobosins had excellent in vitro antifungal activities against <i>B. cinerea</i>. |
| format | Article |
| id | doaj-art-a05806c587c74bf7bf5b701fca756c05 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-a05806c587c74bf7bf5b701fca756c052025-08-20T03:35:28ZengMDPI AGJournal of Fungi2309-608X2025-07-0111751110.3390/jof11070511New Chaetoglobosins with Fungicidal Activity from <i>Chaetomium</i> sp. UJN-EF006 Endophytic in <i>Vaccinium bracteatum</i>Luo-Jing Wang0Zong-Yan Ma1Xin-Ling Wang2Kai-Le Wang3Tong Zhang4Rui-Ying Han5Jun-Jiang Li6Jie Bao7Yin-Yin Wang8Hua Zhang9School of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaNine chaetoglobosins (<b>1</b>–<b>9</b>) including five previously undescribed ones (<b>1</b>–<b>5</b>) were obtained from the culture broth of an endophytic fungus (<i>Chaetomium</i> sp. UJN-EF006) isolated from the leaves of <i>Vaccinium bracteatum</i>. The structures of these fungal metabolites were elucidated by spectroscopic methods including mass spectroscopy, nuclear magnetic resonance, single crystal X-ray crystallography, and electronic circular dichroism. To accelerate the development of novel fungicides, all of the isolated chaetoglobosins were evaluated for their antifungal activity against two crop pathogens, <i>Botrytis cinerea</i> and <i>Sclerotinia sclerotiorum</i>. The assay results revealed that chaetoglobosins <b>2</b>, <b>6</b>, <b>7</b>, and <b>9</b> possessed a significant fungicidal effect against <i>B. cinerea</i>, with EC<sub>50</sub> values all below 10 μg/mL. Particularly, the most potent compound, <b>7</b>, was 175- and 96-fold as active as the commercially available fungicides carbendazim (EC<sub>50</sub> 70.11 μg/mL) and azoxystrobin (EC<sub>50</sub> 39.02 μg/mL), respectively. A further observation under scanning electron microscope indicated that compound <b>2</b> could markedly impair the fungal hyphae of <i>B. cinerea</i>. The study demonstrates that the chaetoglobosins had excellent in vitro antifungal activities against <i>B. cinerea</i>.https://www.mdpi.com/2309-608X/11/7/511<i>Chaetomium</i> sp.chaetoglobosinphytopathogenfungicide<i>Botrytis cinerea</i><i>Sclerotinia sclerotiorum</i> |
| spellingShingle | Luo-Jing Wang Zong-Yan Ma Xin-Ling Wang Kai-Le Wang Tong Zhang Rui-Ying Han Jun-Jiang Li Jie Bao Yin-Yin Wang Hua Zhang New Chaetoglobosins with Fungicidal Activity from <i>Chaetomium</i> sp. UJN-EF006 Endophytic in <i>Vaccinium bracteatum</i> Journal of Fungi <i>Chaetomium</i> sp. chaetoglobosin phytopathogen fungicide <i>Botrytis cinerea</i> <i>Sclerotinia sclerotiorum</i> |
| title | New Chaetoglobosins with Fungicidal Activity from <i>Chaetomium</i> sp. UJN-EF006 Endophytic in <i>Vaccinium bracteatum</i> |
| title_full | New Chaetoglobosins with Fungicidal Activity from <i>Chaetomium</i> sp. UJN-EF006 Endophytic in <i>Vaccinium bracteatum</i> |
| title_fullStr | New Chaetoglobosins with Fungicidal Activity from <i>Chaetomium</i> sp. UJN-EF006 Endophytic in <i>Vaccinium bracteatum</i> |
| title_full_unstemmed | New Chaetoglobosins with Fungicidal Activity from <i>Chaetomium</i> sp. UJN-EF006 Endophytic in <i>Vaccinium bracteatum</i> |
| title_short | New Chaetoglobosins with Fungicidal Activity from <i>Chaetomium</i> sp. UJN-EF006 Endophytic in <i>Vaccinium bracteatum</i> |
| title_sort | new chaetoglobosins with fungicidal activity from i chaetomium i sp ujn ef006 endophytic in i vaccinium bracteatum i |
| topic | <i>Chaetomium</i> sp. chaetoglobosin phytopathogen fungicide <i>Botrytis cinerea</i> <i>Sclerotinia sclerotiorum</i> |
| url | https://www.mdpi.com/2309-608X/11/7/511 |
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