Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods
Several assays have been frequently used to estimate antioxidant capacities including ABTS•+, DPPH, and FRAP assays. Xanthohumol (XN), the major prenylated flavonoid contained in beer, witnessed various reports on its antioxidant capacity. We systematically evaluated the antioxidant activity of XN u...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/249485 |
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| author | Xiu-Li Zhang Yong-Dong Zhang Tao Wang Hong-Yun Guo Qi-Ming Liu Hai-Xiang Su |
| author_facet | Xiu-Li Zhang Yong-Dong Zhang Tao Wang Hong-Yun Guo Qi-Ming Liu Hai-Xiang Su |
| author_sort | Xiu-Li Zhang |
| collection | DOAJ |
| description | Several assays have been frequently used to estimate antioxidant capacities including ABTS•+, DPPH, and FRAP assays. Xanthohumol (XN), the major prenylated flavonoid contained in beer, witnessed various reports on its antioxidant capacity. We systematically evaluated the antioxidant activity of XN using three systems, 2,2,-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+) scavenging assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assays, and ferric reducing antioxidant power (FRAP) assays. The results are expressed as Trolox equivalent antioxidant capacity (TEAC). The TEAC of XN was 0.32±0.09 μmol·l−1 by the ABTS assay and 0.27±0.04 μmol·l−1 by the FRAP. Meanwhile, the XN did not show obviously scavenging effect on DPPH radical reaction system. These results showed that different methods in the evaluation of compound antioxidant capicity, there may be a different conclusion. |
| format | Article |
| id | doaj-art-a05577d3874f44b6b5e57aa0e37e79e2 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-a05577d3874f44b6b5e57aa0e37e79e22025-08-20T03:38:08ZengWileyJournal of Chemistry2090-90632090-90712014-01-01201410.1155/2014/249485249485Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical MethodsXiu-Li Zhang0Yong-Dong Zhang1Tao Wang2Hong-Yun Guo3Qi-Ming Liu4Hai-Xiang Su5Gansu Academic Institute for Medical Research, Lanzhou, Gansu 730050, ChinaGansu Academic Institute for Medical Research, Lanzhou, Gansu 730050, ChinaGansu Academic Institute for Medical Research, Lanzhou, Gansu 730050, ChinaGansu Academic Institute for Medical Research, Lanzhou, Gansu 730050, ChinaYumen Tuopu Scientific and Technological Development Co., Ltd., Yumen, Gansu 735000, ChinaGansu Academic Institute for Medical Research, Lanzhou, Gansu 730050, ChinaSeveral assays have been frequently used to estimate antioxidant capacities including ABTS•+, DPPH, and FRAP assays. Xanthohumol (XN), the major prenylated flavonoid contained in beer, witnessed various reports on its antioxidant capacity. We systematically evaluated the antioxidant activity of XN using three systems, 2,2,-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+) scavenging assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assays, and ferric reducing antioxidant power (FRAP) assays. The results are expressed as Trolox equivalent antioxidant capacity (TEAC). The TEAC of XN was 0.32±0.09 μmol·l−1 by the ABTS assay and 0.27±0.04 μmol·l−1 by the FRAP. Meanwhile, the XN did not show obviously scavenging effect on DPPH radical reaction system. These results showed that different methods in the evaluation of compound antioxidant capicity, there may be a different conclusion.http://dx.doi.org/10.1155/2014/249485 |
| spellingShingle | Xiu-Li Zhang Yong-Dong Zhang Tao Wang Hong-Yun Guo Qi-Ming Liu Hai-Xiang Su Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods Journal of Chemistry |
| title | Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods |
| title_full | Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods |
| title_fullStr | Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods |
| title_full_unstemmed | Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods |
| title_short | Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods |
| title_sort | evaluation on antioxidant effect of xanthohumol by different antioxidant capacity analytical methods |
| url | http://dx.doi.org/10.1155/2014/249485 |
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