Heat shock transcription factor HSFB2b negatively regulates plant thermomorphogenesis in Arabidopsis
In order to explore whether heat shock transcription factor (HSF) known to be involved in plant adaptation to extreme heat stress is also involved in plant thermomorphogenesis at warm temperatures, the result of CRISPR/Cas9 gene editing, physiological and biochemical, genetic experiments, and effect...
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Zhejiang University Press
2023-02-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2022.01.112 |
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| author | YAO Ziwei SUN Jingliang LIU Jianxiang LU Haiping |
| author_facet | YAO Ziwei SUN Jingliang LIU Jianxiang LU Haiping |
| author_sort | YAO Ziwei |
| collection | DOAJ |
| description | In order to explore whether heat shock transcription factor (HSF) known to be involved in plant adaptation to extreme heat stress is also involved in plant thermomorphogenesis at warm temperatures, the result of CRISPR/Cas9 gene editing, physiological and biochemical, genetic experiments, and effector-reporter assay demonstrated that the heat shock transcription factor HSFB2b was induced by the warm temperature and played an important role in the process of plant thermomorphogenesis. Under the warm temperature (29 ℃), the Arabidopsis mutant hsfb2b exhibited a longer hypocotyl than the wild type, suggesting that HSFB2b functioned as a negative regulator in thermomorphogenesis. Subcellular localization results showed that the HSFB2b protein was localized in the nucleus. Real-time quantitative polymerase chain reaction (qRT-PCR) analysis showed that the heat shock proteins (HSPs) gene, the heat shock transcription factor HSFA2, and the jasmonic acid degradation gene ST2A were up-regulated in the wild type under the warm temperature relative to the normal temperature (22 ℃), but these genes were more up-regulated by the warm temperature in the hsfb2b mutant than that in the wild type. Furthermore, effector-reporter assay demonstrated that HSFB2b could inhibit ST2A expression by binding to the heat shock element (HSE). In conclusion, the heat shock transcription factor HSFB2b induced by the warm temperature played a negative regulatory role in the hypocotyl elongation and negatively regulated the expression of gene ST2A by recognizing the HSE in molecular mechanism, thus negatively regulated the plant thermomorphogenesis. |
| format | Article |
| id | doaj-art-a0539ca777624161bb541d1e4a4583a5 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-a0539ca777624161bb541d1e4a4583a52025-08-20T03:32:04ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552023-02-0149233010.3785/j.issn.1008-9209.2022.01.11210089209Heat shock transcription factor HSFB2b negatively regulates plant thermomorphogenesis in ArabidopsisYAO ZiweiSUN JingliangLIU JianxiangLU HaipingIn order to explore whether heat shock transcription factor (HSF) known to be involved in plant adaptation to extreme heat stress is also involved in plant thermomorphogenesis at warm temperatures, the result of CRISPR/Cas9 gene editing, physiological and biochemical, genetic experiments, and effector-reporter assay demonstrated that the heat shock transcription factor HSFB2b was induced by the warm temperature and played an important role in the process of plant thermomorphogenesis. Under the warm temperature (29 ℃), the Arabidopsis mutant hsfb2b exhibited a longer hypocotyl than the wild type, suggesting that HSFB2b functioned as a negative regulator in thermomorphogenesis. Subcellular localization results showed that the HSFB2b protein was localized in the nucleus. Real-time quantitative polymerase chain reaction (qRT-PCR) analysis showed that the heat shock proteins (HSPs) gene, the heat shock transcription factor HSFA2, and the jasmonic acid degradation gene ST2A were up-regulated in the wild type under the warm temperature relative to the normal temperature (22 ℃), but these genes were more up-regulated by the warm temperature in the hsfb2b mutant than that in the wild type. Furthermore, effector-reporter assay demonstrated that HSFB2b could inhibit ST2A expression by binding to the heat shock element (HSE). In conclusion, the heat shock transcription factor HSFB2b induced by the warm temperature played a negative regulatory role in the hypocotyl elongation and negatively regulated the expression of gene ST2A by recognizing the HSE in molecular mechanism, thus negatively regulated the plant thermomorphogenesis.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2022.01.112<italic>Arabidopsis</italic> thermomorphogenesisheat shock transcription factorjasmonic acid catabolismhypocotyl elongation |
| spellingShingle | YAO Ziwei SUN Jingliang LIU Jianxiang LU Haiping Heat shock transcription factor HSFB2b negatively regulates plant thermomorphogenesis in Arabidopsis 浙江大学学报. 农业与生命科学版 <italic>Arabidopsis</italic> thermomorphogenesis heat shock transcription factor jasmonic acid catabolism hypocotyl elongation |
| title | Heat shock transcription factor HSFB2b negatively regulates plant thermomorphogenesis in Arabidopsis |
| title_full | Heat shock transcription factor HSFB2b negatively regulates plant thermomorphogenesis in Arabidopsis |
| title_fullStr | Heat shock transcription factor HSFB2b negatively regulates plant thermomorphogenesis in Arabidopsis |
| title_full_unstemmed | Heat shock transcription factor HSFB2b negatively regulates plant thermomorphogenesis in Arabidopsis |
| title_short | Heat shock transcription factor HSFB2b negatively regulates plant thermomorphogenesis in Arabidopsis |
| title_sort | heat shock transcription factor hsfb2b negatively regulates plant thermomorphogenesis in arabidopsis |
| topic | <italic>Arabidopsis</italic> thermomorphogenesis heat shock transcription factor jasmonic acid catabolism hypocotyl elongation |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2022.01.112 |
| work_keys_str_mv | AT yaoziwei heatshocktranscriptionfactorhsfb2bnegativelyregulatesplantthermomorphogenesisinarabidopsis AT sunjingliang heatshocktranscriptionfactorhsfb2bnegativelyregulatesplantthermomorphogenesisinarabidopsis AT liujianxiang heatshocktranscriptionfactorhsfb2bnegativelyregulatesplantthermomorphogenesisinarabidopsis AT luhaiping heatshocktranscriptionfactorhsfb2bnegativelyregulatesplantthermomorphogenesisinarabidopsis |