Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects
Abstract Nanocatalysis coupled with photothermal therapy is a potent anti-cancer approach, yet its clinical utility is limited by low concentration of tumor substrate, redox interference, and risks of overheating normal tissues. Herein, we propose an innovative closed-loop nanozyme approach that lev...
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BMC
2025-04-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03320-2 |
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| author | Fan Yang Chunyu Yan Nannan Li Xinxiu Jiang Baojie Du Peirong Bai Liping Li Ruiping Zhang |
| author_facet | Fan Yang Chunyu Yan Nannan Li Xinxiu Jiang Baojie Du Peirong Bai Liping Li Ruiping Zhang |
| author_sort | Fan Yang |
| collection | DOAJ |
| description | Abstract Nanocatalysis coupled with photothermal therapy is a potent anti-cancer approach, yet its clinical utility is limited by low concentration of tumor substrate, redox interference, and risks of overheating normal tissues. Herein, we propose an innovative closed-loop nanozyme approach that leverages the synergistic effects of catalytic and mild photothermal therapy (mPTT) to address aforementioned challenges. The strategy features a folic acid-functionalized iron single-atom catalyst (FeNC-FA), designed to exhibit exceptional multienzymatic capabilities and an optimal photothermal response. In the system, the engineered FeNC-FA is capable of inducing reactive oxygen species (ROS) storm and depleting glutathione (GSH) in the specific tumor microenvironment (TME) to initiate ferroptosis. Concurrently, the accumulation of ROS effectively cleaves heat shock proteins (HSPs), thereby enhancing mPTT. An intriguing aspect is that the increased temperature within the TME further facilitates the conversion of H2O2 to O2, alleviating hypoxia and providing a positive feedback circuit to boost catalytic therapy. Additionally, the advanced photoacoustic (PA) imaging capabilities of FeNC-FA allow for self-monitoring of their accumulation at tumor sites, thereby guiding the mPTT process. Taken together, it provides a PA image-guided, mutually reinforced catalytic and mild photothermal synergistic tumor therapy both in vitro and in vivo. This targeted and synergistic strategy holds great promise for personalized medicine applications. |
| format | Article |
| id | doaj-art-a040aa0ceff142618bc94e1b06427a96 |
| institution | OA Journals |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-a040aa0ceff142618bc94e1b06427a962025-08-20T02:25:41ZengBMCJournal of Nanobiotechnology1477-31552025-04-0123111110.1186/s12951-025-03320-2Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effectsFan Yang0Chunyu Yan1Nannan Li2Xinxiu Jiang3Baojie Du4Peirong Bai5Liping Li6Ruiping Zhang7Basic Medical College, Research Institute of Circadian Rhythm and Disease, Academy of Medical Sciences, Shanxi Medical UniversityBasic Medical College, Research Institute of Circadian Rhythm and Disease, Academy of Medical Sciences, Shanxi Medical UniversityBasic Medical College, Research Institute of Circadian Rhythm and Disease, Academy of Medical Sciences, Shanxi Medical UniversityBasic Medical College, Research Institute of Circadian Rhythm and Disease, Academy of Medical Sciences, Shanxi Medical UniversityShanxi Bethune Hospital, Third Hospital of Shanxi Medical University, Shanxi Academy of Medical Sciences, Tongji Shanxi HospitalShanxi Bethune Hospital, Third Hospital of Shanxi Medical University, Shanxi Academy of Medical Sciences, Tongji Shanxi HospitalBasic Medical College, Research Institute of Circadian Rhythm and Disease, Academy of Medical Sciences, Shanxi Medical UniversityThe Radiology Department of Shanxi Provincial People’s Hospital, Five Hospital of Shanxi Medical UniversityAbstract Nanocatalysis coupled with photothermal therapy is a potent anti-cancer approach, yet its clinical utility is limited by low concentration of tumor substrate, redox interference, and risks of overheating normal tissues. Herein, we propose an innovative closed-loop nanozyme approach that leverages the synergistic effects of catalytic and mild photothermal therapy (mPTT) to address aforementioned challenges. The strategy features a folic acid-functionalized iron single-atom catalyst (FeNC-FA), designed to exhibit exceptional multienzymatic capabilities and an optimal photothermal response. In the system, the engineered FeNC-FA is capable of inducing reactive oxygen species (ROS) storm and depleting glutathione (GSH) in the specific tumor microenvironment (TME) to initiate ferroptosis. Concurrently, the accumulation of ROS effectively cleaves heat shock proteins (HSPs), thereby enhancing mPTT. An intriguing aspect is that the increased temperature within the TME further facilitates the conversion of H2O2 to O2, alleviating hypoxia and providing a positive feedback circuit to boost catalytic therapy. Additionally, the advanced photoacoustic (PA) imaging capabilities of FeNC-FA allow for self-monitoring of their accumulation at tumor sites, thereby guiding the mPTT process. Taken together, it provides a PA image-guided, mutually reinforced catalytic and mild photothermal synergistic tumor therapy both in vitro and in vivo. This targeted and synergistic strategy holds great promise for personalized medicine applications.https://doi.org/10.1186/s12951-025-03320-2Single-atom nanozymeNanocatalytic therapyPA imaging guided-synergistic therapyMild-temperature photothermal therapyClosed-loop strategy |
| spellingShingle | Fan Yang Chunyu Yan Nannan Li Xinxiu Jiang Baojie Du Peirong Bai Liping Li Ruiping Zhang Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects Journal of Nanobiotechnology Single-atom nanozyme Nanocatalytic therapy PA imaging guided-synergistic therapy Mild-temperature photothermal therapy Closed-loop strategy |
| title | Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects |
| title_full | Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects |
| title_fullStr | Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects |
| title_full_unstemmed | Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects |
| title_short | Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects |
| title_sort | closed loop cascade nanozyme strategy for mutually reinforced catalytic and mild temperature photothermal therapeutic effects |
| topic | Single-atom nanozyme Nanocatalytic therapy PA imaging guided-synergistic therapy Mild-temperature photothermal therapy Closed-loop strategy |
| url | https://doi.org/10.1186/s12951-025-03320-2 |
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