The analysis of antimicrobials epidemiological cut-off values of mycoplasma gallisepticum isolated from goose

Mycoplasma gallisepticum (MG) poses a significant threat to the goose breeding industry, with antimicrobial agents commonly employed for its treatment. However, the absence of standardized criteria for antimicrobial selection complicates clinical decision-making regarding the choice and dosage of th...

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Main Authors: Yangshuo Li, Jingyi Liang, Weihuo Li, Yixin Jia, Sheng Yuan, Yuanyuan Zhou, Yuee Hou, Nan Zhang
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125002135
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author Yangshuo Li
Jingyi Liang
Weihuo Li
Yixin Jia
Sheng Yuan
Yuanyuan Zhou
Yuee Hou
Nan Zhang
author_facet Yangshuo Li
Jingyi Liang
Weihuo Li
Yixin Jia
Sheng Yuan
Yuanyuan Zhou
Yuee Hou
Nan Zhang
author_sort Yangshuo Li
collection DOAJ
description Mycoplasma gallisepticum (MG) poses a significant threat to the goose breeding industry, with antimicrobial agents commonly employed for its treatment. However, the absence of standardized criteria for antimicrobial selection complicates clinical decision-making regarding the choice and dosage of these agents, contributing to the emergence of clinical resistance. In this study, we isolated 102 clinical strains of MG from geese in Guangdong. We determined the minimum inhibitory concentration (MIC) values for various antimicrobials frequently used in MG treatment, including danofloxacin, enrofloxacin, tilmicosin, tylosin, tylvalosin, valnemulin, tiamulin, and spectinomycin. After conducting a statistical analysis of the susceptibility test results, we employed ECOFFinder to establish the wild-type cutoff values (ECOFFs) for these antimicrobials against the MG isolates. The findings revealed a significant reduction in sensitivity among the clinical isolates to the eight tested antimicrobial agents. While valnemulin and tiamulin maintained relatively good sensitivity, enrofloxacin, danofloxacin, and spectinomycin exhibited notably diminished effectiveness. The determined ECOFFs for MG isolated from geese against the aforementioned antimicrobials were 3.2, 3.2, 0.8, 0.8, 0.08, 0.05, 0.05, and 3.2 μg/mL, respectively. This study is crucial for identifying resistant strains and investigating resistance mechanisms, in addition to providing a precise assessment of the resistance levels of MG isolated from geese in Guangdong. The insights gained from this research will serve as a valuable reference for the prevention and treatment of MG infections and will promote more effective clinical strategies regarding the use of antimicrobials.
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spelling doaj-art-a0406fa47a3b41ca87702db9be7fdd682025-08-20T02:30:59ZengElsevierPoultry Science0032-57912025-05-01104510497410.1016/j.psj.2025.104974The analysis of antimicrobials epidemiological cut-off values of mycoplasma gallisepticum isolated from gooseYangshuo Li0Jingyi Liang1Weihuo Li2Yixin Jia3Sheng Yuan4Yuanyuan Zhou5Yuee Hou6Nan Zhang7School of Animal Science and Technology, Foshan University, Foshan, PR ChinaSchool of Animal Science and Technology, Foshan University, Foshan, PR ChinaSchool of Animal Science and Technology, Foshan University, Foshan, PR ChinaSchool of Animal Science and Technology, Foshan University, Foshan, PR ChinaSchool of Animal Science and Technology, Foshan University, Foshan, PR ChinaSchool of Animal Science and Technology, Foshan University, Foshan, PR ChinaZhuhai Kerric Testing, Zhuhai, PR ChinaSchool of Animal Science and Technology, Foshan University, Foshan, PR China; Corresponding author at. School of Animal Science and Technology, Foshan University, Foshan, 528225, PR China.Mycoplasma gallisepticum (MG) poses a significant threat to the goose breeding industry, with antimicrobial agents commonly employed for its treatment. However, the absence of standardized criteria for antimicrobial selection complicates clinical decision-making regarding the choice and dosage of these agents, contributing to the emergence of clinical resistance. In this study, we isolated 102 clinical strains of MG from geese in Guangdong. We determined the minimum inhibitory concentration (MIC) values for various antimicrobials frequently used in MG treatment, including danofloxacin, enrofloxacin, tilmicosin, tylosin, tylvalosin, valnemulin, tiamulin, and spectinomycin. After conducting a statistical analysis of the susceptibility test results, we employed ECOFFinder to establish the wild-type cutoff values (ECOFFs) for these antimicrobials against the MG isolates. The findings revealed a significant reduction in sensitivity among the clinical isolates to the eight tested antimicrobial agents. While valnemulin and tiamulin maintained relatively good sensitivity, enrofloxacin, danofloxacin, and spectinomycin exhibited notably diminished effectiveness. The determined ECOFFs for MG isolated from geese against the aforementioned antimicrobials were 3.2, 3.2, 0.8, 0.8, 0.08, 0.05, 0.05, and 3.2 μg/mL, respectively. This study is crucial for identifying resistant strains and investigating resistance mechanisms, in addition to providing a precise assessment of the resistance levels of MG isolated from geese in Guangdong. The insights gained from this research will serve as a valuable reference for the prevention and treatment of MG infections and will promote more effective clinical strategies regarding the use of antimicrobials.http://www.sciencedirect.com/science/article/pii/S0032579125002135Antibiotic resistanceGooseMycoplasma gallisepticumWild-type folds
spellingShingle Yangshuo Li
Jingyi Liang
Weihuo Li
Yixin Jia
Sheng Yuan
Yuanyuan Zhou
Yuee Hou
Nan Zhang
The analysis of antimicrobials epidemiological cut-off values of mycoplasma gallisepticum isolated from goose
Poultry Science
Antibiotic resistance
Goose
Mycoplasma gallisepticum
Wild-type folds
title The analysis of antimicrobials epidemiological cut-off values of mycoplasma gallisepticum isolated from goose
title_full The analysis of antimicrobials epidemiological cut-off values of mycoplasma gallisepticum isolated from goose
title_fullStr The analysis of antimicrobials epidemiological cut-off values of mycoplasma gallisepticum isolated from goose
title_full_unstemmed The analysis of antimicrobials epidemiological cut-off values of mycoplasma gallisepticum isolated from goose
title_short The analysis of antimicrobials epidemiological cut-off values of mycoplasma gallisepticum isolated from goose
title_sort analysis of antimicrobials epidemiological cut off values of mycoplasma gallisepticum isolated from goose
topic Antibiotic resistance
Goose
Mycoplasma gallisepticum
Wild-type folds
url http://www.sciencedirect.com/science/article/pii/S0032579125002135
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