Effect of TRAF-interacting protein with FHA domain-containing protein A on acute kidney injury during sepsis by regulating pyroptosis through NF-κB

Effect of TRAF-interacting protein with FHA domain-containing protein A on acute kidney injury during sepsis by regulating pyroptosis through NF-κB

ObjectiveTo explore the effect of TRAF-interacting protein with FHA domain-containing protein A （TIFA） on pyroptosis of renal tubular epithelial cells and examine whether or not it operates through NF-κB signaling pathway.MethodsBlood specimens were collected from 12 septic patients with acute kidne...

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Bibliographic Details
Main Authors: Li Yi-ming, Qian Yao-yao, Suo Jin-meng, Hu Ya-nan, Peng Zhi-yong
Format: Article
Language:zho
Published: Editorial Department of Journal of Clinical Nephrology 2022-07-01
Series:Linchuang shenzangbing zazhi
Subjects:
Sepsis
Acute kidney injury
Renal tubular epithelial cell
Pyroptosis
Online Access:http://www.lcszb.com/thesisDetails#10.3969/j.issn.1671-2390.2022.07.009
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Summary:ObjectiveTo explore the effect of TRAF-interacting protein with FHA domain-containing protein A （TIFA） on pyroptosis of renal tubular epithelial cells and examine whether or not it operates through NF-κB signaling pathway.MethodsBlood specimens were collected from 12 septic patients with acute kidney injury （AKI） in intensive care unit （ICU）. The levels of creatinine and TIFA were measured. The expressions of TIFA， caspase-1 and GSDMD were detected by Western blot and mitochondrial membrane potential by JC-1. Septic model was established by cecal ligation and puncture and the mice received an intraperitoneal injection of TLR4 inhibitor for detecting renal injury and the expression of TIFA.ResultsUrinary TIFA rose after renal injury in septic patients. In renal tubular epithelial cells， a knockdown of TIFA could suppress the expressions of Caspase-1 and GSDMD and minimize the change of mitochondrial membrane potential. In septic AKI mice， an inhibition of TLR4 signaling pathway could suppress TIFA and NF-κB， reduce the production of inflammatory cytokines IL-1β and IL-18 and improve renal pathology and survival rate.ConclusionTIFA may reduce pyroptosis by inhibiting NF-κB signaling pathway in septic AKI. Targeted treatment of TIFA may improve septic AKI.
ISSN:1671-2390

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