Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions
Hydrogel network structures play a crucial role in determining mechanical properties and have broad applications in biomedical and industrial fields. Therefore, their rational design is essential. Herein, we developed a Schiff base-crosslinked hydrogel through the reaction of Tetra-armed polyethylen...
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| Language: | English |
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MDPI AG
2025-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/16/2/69 |
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| author | Yuanyuan Qu Jinlong Li Xin Jia Lijun Yin |
| author_facet | Yuanyuan Qu Jinlong Li Xin Jia Lijun Yin |
| author_sort | Yuanyuan Qu |
| collection | DOAJ |
| description | Hydrogel network structures play a crucial role in determining mechanical properties and have broad applications in biomedical and industrial fields. Therefore, their rational design is essential. Herein, we developed a Schiff base-crosslinked hydrogel through the reaction of Tetra-armed polyethylene glycol with aldehyde end groups (Tetra-PEG-CHO) and bovine serum albumin (BSA) under alkaline conditions. In addition, the Tetra-PEG-BSA hydrogel showed a rapid gelation time of around 11 s, much faster than that of the GLU-BSA, HT-BSA, and GDL-BSA hydrogels. It had high optical transmittance (92.92% at 600 nm) and swelling ratios superior to the other gels in different solutions, maintaining structural integrity even in denaturing environments such as guanidine hydrochloride and SDS. Mechanical tests showed superior strain at break (84.12 ± 0.76%), rupture stress (28.64 ± 1.21 kPa), and energy dissipation ability (468.0 ± 34.9 kJ·m<sup>−3</sup>), surpassing all control group hydrogels. MTT cytotoxicity assays indicated that cell viability remained >80% at lower concentrations, confirming excellent biocompatibility. These findings suggest that Tetra-PEG-BSA hydrogels may serve as effective materials for drug delivery, tissue engineering, and 3D printing. |
| format | Article |
| id | doaj-art-a02d43902ec74656a43c1c26d9f8bf6a |
| institution | DOAJ |
| issn | 2079-4983 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj-art-a02d43902ec74656a43c1c26d9f8bf6a2025-08-20T03:12:08ZengMDPI AGJournal of Functional Biomaterials2079-49832025-02-011626910.3390/jfb16020069Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional FunctionsYuanyuan Qu0Jinlong Li1Xin Jia2Lijun Yin3College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua Donglu, Haidian District, Beijing 100083, ChinaCollege of Chemistry and Materials Engineering, Beijing Technology and Business University, 11 Fucheng Road, Haidian District, Beijing 100048, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua Donglu, Haidian District, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua Donglu, Haidian District, Beijing 100083, ChinaHydrogel network structures play a crucial role in determining mechanical properties and have broad applications in biomedical and industrial fields. Therefore, their rational design is essential. Herein, we developed a Schiff base-crosslinked hydrogel through the reaction of Tetra-armed polyethylene glycol with aldehyde end groups (Tetra-PEG-CHO) and bovine serum albumin (BSA) under alkaline conditions. In addition, the Tetra-PEG-BSA hydrogel showed a rapid gelation time of around 11 s, much faster than that of the GLU-BSA, HT-BSA, and GDL-BSA hydrogels. It had high optical transmittance (92.92% at 600 nm) and swelling ratios superior to the other gels in different solutions, maintaining structural integrity even in denaturing environments such as guanidine hydrochloride and SDS. Mechanical tests showed superior strain at break (84.12 ± 0.76%), rupture stress (28.64 ± 1.21 kPa), and energy dissipation ability (468.0 ± 34.9 kJ·m<sup>−3</sup>), surpassing all control group hydrogels. MTT cytotoxicity assays indicated that cell viability remained >80% at lower concentrations, confirming excellent biocompatibility. These findings suggest that Tetra-PEG-BSA hydrogels may serve as effective materials for drug delivery, tissue engineering, and 3D printing.https://www.mdpi.com/2079-4983/16/2/69hydrogelSchiff baseBSATetra-PEGprotein |
| spellingShingle | Yuanyuan Qu Jinlong Li Xin Jia Lijun Yin Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions Journal of Functional Biomaterials hydrogel Schiff base BSA Tetra-PEG protein |
| title | Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions |
| title_full | Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions |
| title_fullStr | Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions |
| title_full_unstemmed | Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions |
| title_short | Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions |
| title_sort | schiff base crosslinked tetra peg bsa hydrogel design properties and multifunctional functions |
| topic | hydrogel Schiff base BSA Tetra-PEG protein |
| url | https://www.mdpi.com/2079-4983/16/2/69 |
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