Bioreactor-Based Liquid Culture and Production of Konjac Micro-Corm
Konjac (<i>Amorphophallus konjac</i> K. Koch) has numerous health benefits, but traditional propagation is hindered by long growth periods and soil-borne diseases. This study developed a novel cell liquid culture system to directly produce micro-corms of konjac for large-scale production...
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MDPI AG
2025-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/11/3/235 |
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| author | Yuqi Sun Xian Sun Yufan Pan Changbin Liu Lingye Su Zongshen Zhang |
| author_facet | Yuqi Sun Xian Sun Yufan Pan Changbin Liu Lingye Su Zongshen Zhang |
| author_sort | Yuqi Sun |
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| description | Konjac (<i>Amorphophallus konjac</i> K. Koch) has numerous health benefits, but traditional propagation is hindered by long growth periods and soil-borne diseases. This study developed a novel cell liquid culture system to directly produce micro-corms of konjac for large-scale production. The results demonstrated significant improvements in bud induction and rooting compared to solid culture. Under MS + 1.0 mg/L 6-BA + 0.5 mg/L NAA, the induced buds per culture vessel and final fresh weight were 24.87 ± 0.06 and 6.64 ± 0.12 g, respectively, 1.95 and 1.67 times higher than those in solid culture. Rooting experiments showed that 1/2 MS + 0.5 mg/L IBA + 1.0 mg/L NAA resulted in a root length of 25.23 ± 0.04 cm and 18.12 ± 0.01 roots per vessel. Using a 5 L bioreactor for micro-corm induction led to a 2.51-fold increase in fresh weight (52.67 ± 0.01 g) after 31 days, with glucomannan production reaching 0.48 g/g <sub>(fresh weight)</sub>. The optimized culture system also significantly reduced the propagation time and increased the yield of healthy micro-corms. Bioreactor-based cultivation effectively enhances konjac induction efficiency and shortens breeding time, making it a promising approach for commercial production of konjac micro-corms and potentially improving the economic viability of konjac farming. |
| format | Article |
| id | doaj-art-a02ba4fa5f8a4cc4975379a751a757f0 |
| institution | OA Journals |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-a02ba4fa5f8a4cc4975379a751a757f02025-08-20T02:11:11ZengMDPI AGHorticulturae2311-75242025-02-0111323510.3390/horticulturae11030235Bioreactor-Based Liquid Culture and Production of Konjac Micro-CormYuqi Sun0Xian Sun1Yufan Pan2Changbin Liu3Lingye Su4Zongshen Zhang5Laboratory of Pharmaceutical Plant Cell Culture Research, School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, ChinaLaboratory of Pharmaceutical Plant Cell Culture Research, School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, ChinaLaboratory of Pharmaceutical Plant Cell Culture Research, School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, ChinaLaboratory of Pharmaceutical Plant Cell Culture Research, School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, ChinaGuangdong Provincial Key Laboratory of Silviculture Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520, ChinaLaboratory of Pharmaceutical Plant Cell Culture Research, School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, ChinaKonjac (<i>Amorphophallus konjac</i> K. Koch) has numerous health benefits, but traditional propagation is hindered by long growth periods and soil-borne diseases. This study developed a novel cell liquid culture system to directly produce micro-corms of konjac for large-scale production. The results demonstrated significant improvements in bud induction and rooting compared to solid culture. Under MS + 1.0 mg/L 6-BA + 0.5 mg/L NAA, the induced buds per culture vessel and final fresh weight were 24.87 ± 0.06 and 6.64 ± 0.12 g, respectively, 1.95 and 1.67 times higher than those in solid culture. Rooting experiments showed that 1/2 MS + 0.5 mg/L IBA + 1.0 mg/L NAA resulted in a root length of 25.23 ± 0.04 cm and 18.12 ± 0.01 roots per vessel. Using a 5 L bioreactor for micro-corm induction led to a 2.51-fold increase in fresh weight (52.67 ± 0.01 g) after 31 days, with glucomannan production reaching 0.48 g/g <sub>(fresh weight)</sub>. The optimized culture system also significantly reduced the propagation time and increased the yield of healthy micro-corms. Bioreactor-based cultivation effectively enhances konjac induction efficiency and shortens breeding time, making it a promising approach for commercial production of konjac micro-corms and potentially improving the economic viability of konjac farming.https://www.mdpi.com/2311-7524/11/3/235<i>Amorphophallus</i>bioreactor breedingliquid culture |
| spellingShingle | Yuqi Sun Xian Sun Yufan Pan Changbin Liu Lingye Su Zongshen Zhang Bioreactor-Based Liquid Culture and Production of Konjac Micro-Corm Horticulturae <i>Amorphophallus</i> bioreactor breeding liquid culture |
| title | Bioreactor-Based Liquid Culture and Production of Konjac Micro-Corm |
| title_full | Bioreactor-Based Liquid Culture and Production of Konjac Micro-Corm |
| title_fullStr | Bioreactor-Based Liquid Culture and Production of Konjac Micro-Corm |
| title_full_unstemmed | Bioreactor-Based Liquid Culture and Production of Konjac Micro-Corm |
| title_short | Bioreactor-Based Liquid Culture and Production of Konjac Micro-Corm |
| title_sort | bioreactor based liquid culture and production of konjac micro corm |
| topic | <i>Amorphophallus</i> bioreactor breeding liquid culture |
| url | https://www.mdpi.com/2311-7524/11/3/235 |
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