Rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a Japanese population

Abstract Atrial fibrillation (AF) is the most prevalent arrhythmia in the world and can cause serious complications such as stroke or heart failure. Paroxysmal atrial fibrillation (PAF), a subtype of AF, accounts for approximately 25% of AF cases and is estimated to affect approximately 30 million p...

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Main Authors: Kanji Tabata, Takeaki Sudo, Yuki Nagata, Kensuke Ihara, Ken Asada, Atsuhiro Kinoshita, Yasuaki Tanaka, Yasuteru Yamauchi, Takeshi Sasaki, Hitoshi Hachiya, Yasushi Imai, Hideo Fujita, Tetsuo Sasano, Tetsushi Furukawa, Takanori Iwata, Toshihiro Tanaka
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-97794-7
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author Kanji Tabata
Takeaki Sudo
Yuki Nagata
Kensuke Ihara
Ken Asada
Atsuhiro Kinoshita
Yasuaki Tanaka
Yasuteru Yamauchi
Takeshi Sasaki
Hitoshi Hachiya
Yasushi Imai
Hideo Fujita
Tetsuo Sasano
Tetsushi Furukawa
Takanori Iwata
Toshihiro Tanaka
author_facet Kanji Tabata
Takeaki Sudo
Yuki Nagata
Kensuke Ihara
Ken Asada
Atsuhiro Kinoshita
Yasuaki Tanaka
Yasuteru Yamauchi
Takeshi Sasaki
Hitoshi Hachiya
Yasushi Imai
Hideo Fujita
Tetsuo Sasano
Tetsushi Furukawa
Takanori Iwata
Toshihiro Tanaka
author_sort Kanji Tabata
collection DOAJ
description Abstract Atrial fibrillation (AF) is the most prevalent arrhythmia in the world and can cause serious complications such as stroke or heart failure. Paroxysmal atrial fibrillation (PAF), a subtype of AF, accounts for approximately 25% of AF cases and is estimated to affect approximately 30 million people worldwide. Despite extensive genetic research on AF, the genetic factors involved in PAF in East Asian (EAS) populations remain unidentified. The aim of our study was to identify genetic factors associated with PAF in the Japanese population, contributing to our understanding of the genetic architecture of AF in Japanese populations. We conducted whole-exome sequencing on a cohort of 1176 PAF individuals and 1172 non-PAF control subjects in a Japanese population. We processed the sequencing data in accordance with the best practices outlined in the Genome Analysis Toolkit (GATK) and conducted gene-based association tests under three variant grouping strategies (masks) using the burden test, SKAT, and SKAT-O. We then performed a meta-analysis of the resulting P-values, which revealed that four genes—ZNF785, SMPD3, GFRA4, and LGALS1—were significantly associated with PAF, representing novel findings. These findings provide new insights into PAF pathogenesis and suggest potential biomarkers for early detection.
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spelling doaj-art-a02b51e1b215455b970fbe45ca03b3ab2025-08-20T03:18:41ZengNature PortfolioScientific Reports2045-23222025-04-0115111010.1038/s41598-025-97794-7Rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a Japanese populationKanji Tabata0Takeaki Sudo1Yuki Nagata2Kensuke Ihara3Ken Asada4Atsuhiro Kinoshita5Yasuaki Tanaka6Yasuteru Yamauchi7Takeshi Sasaki8Hitoshi Hachiya9Yasushi Imai10Hideo Fujita11Tetsuo Sasano12Tetsushi Furukawa13Takanori Iwata14Toshihiro Tanaka15Department of Human Genetics and Disease Diversity, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo)Department of Educational Media Development, Institute of Science Tokyo (Science Tokyo)Department of Human Genetics and Disease Diversity, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo)Department of Bio-Informational Pharmacology, Medicine Research Institute, Institute of Science Tokyo (Science Tokyo)Cancer Translational Research Team, RIKEN Center for Advanced Intelligence ProjectDepartment of Educational Media Development, Institute of Science Tokyo (Science Tokyo)Department of Cardiology, Yokosuka Kyosai HospitalDepartment of Cardiology, Yokohama City Minato Red Cross HospitalDepartment of Cardiology, Heart Rhythm Center, National Hospital Organization Disaster Medical CenterCardiology Division, Cardiovascular Center, Tsuchiura Kyodo General HospitalDivision of Clinical Pharmacology, Department of Pharmacology, Jichi Medical UniversityDivision of Cardiovascular Medicine, Saitama Medical Center, Jichi Medical UniversityDepartment of Cardiovascular Medicine, Institute of Science Tokyo (Science Tokyo)Department of Bio-Informational Pharmacology, Medicine Research Institute, Institute of Science Tokyo (Science Tokyo)Department of Periodontology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo)Department of Human Genetics and Disease Diversity, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo)Abstract Atrial fibrillation (AF) is the most prevalent arrhythmia in the world and can cause serious complications such as stroke or heart failure. Paroxysmal atrial fibrillation (PAF), a subtype of AF, accounts for approximately 25% of AF cases and is estimated to affect approximately 30 million people worldwide. Despite extensive genetic research on AF, the genetic factors involved in PAF in East Asian (EAS) populations remain unidentified. The aim of our study was to identify genetic factors associated with PAF in the Japanese population, contributing to our understanding of the genetic architecture of AF in Japanese populations. We conducted whole-exome sequencing on a cohort of 1176 PAF individuals and 1172 non-PAF control subjects in a Japanese population. We processed the sequencing data in accordance with the best practices outlined in the Genome Analysis Toolkit (GATK) and conducted gene-based association tests under three variant grouping strategies (masks) using the burden test, SKAT, and SKAT-O. We then performed a meta-analysis of the resulting P-values, which revealed that four genes—ZNF785, SMPD3, GFRA4, and LGALS1—were significantly associated with PAF, representing novel findings. These findings provide new insights into PAF pathogenesis and suggest potential biomarkers for early detection.https://doi.org/10.1038/s41598-025-97794-7Paroxysmal atrial fibrillationExome sequencingGene-based testsJapanese population
spellingShingle Kanji Tabata
Takeaki Sudo
Yuki Nagata
Kensuke Ihara
Ken Asada
Atsuhiro Kinoshita
Yasuaki Tanaka
Yasuteru Yamauchi
Takeshi Sasaki
Hitoshi Hachiya
Yasushi Imai
Hideo Fujita
Tetsuo Sasano
Tetsushi Furukawa
Takanori Iwata
Toshihiro Tanaka
Rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a Japanese population
Scientific Reports
Paroxysmal atrial fibrillation
Exome sequencing
Gene-based tests
Japanese population
title Rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a Japanese population
title_full Rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a Japanese population
title_fullStr Rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a Japanese population
title_full_unstemmed Rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a Japanese population
title_short Rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a Japanese population
title_sort rare genetic variants involved in increased risk of paroxysmal atrial fibrillation in a japanese population
topic Paroxysmal atrial fibrillation
Exome sequencing
Gene-based tests
Japanese population
url https://doi.org/10.1038/s41598-025-97794-7
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