Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight
Bacterial leaf blight in rice is a significant disease, and understanding the effects of straw burning on pathogens and antibiotic resistance genes (ARGs) in paddy fields is crucial. Soil and rice stubble samples were collected from bacterial leaf blight outbreak areas in Ningbo City of Zhejiang Pro...
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Zhejiang University Press
2023-12-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.01.131 |
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| author | WANG Binhao CHEN Yanbo LIU Rongjie WANG Dongqun DONG Zuozhen DI Rui WANG Xiao WU Yuping |
| author_facet | WANG Binhao CHEN Yanbo LIU Rongjie WANG Dongqun DONG Zuozhen DI Rui WANG Xiao WU Yuping |
| author_sort | WANG Binhao |
| collection | DOAJ |
| description | Bacterial leaf blight in rice is a significant disease, and understanding the effects of straw burning on pathogens and antibiotic resistance genes (ARGs) in paddy fields is crucial. Soil and rice stubble samples were collected from bacterial leaf blight outbreak areas in Ningbo City of Zhejiang Province, and these samples were subjected to high-throughput metagenomic sequencing to investigate the effects of straw burning. The results indicated that the relative abundance of pathogens in the rice stubble was significantly higher than that in the soil. After straw burning, except for the significant increase in available potassium content, there were no significant changes in the other physicochemical properties of the soil. Overall, there were no significant differences in pathogen community diversity after straw burning, but straw burning led to a sharp decrease in the relative abundance of Xanthomonas oryzae in the rice stubble. The relative abundances of ARGs in the soil and rice stubble increased after straw burning, but there were no significant differences compared with those before straw burning (P＞0.05), while the relative abundance of mobile genetic elements (MGEs) in the rice stubble decreased significantly after straw burning (P＜0.05). The proportion of positively correlated links in the coexisting networks of pathogens-ARGs decreased after straw burning. Mantel analysis revealed that the water content (r=0.642, p＜0.01), pH value (r=0.582, p＜0.05), total organic carbon content (r=0.325, p＜0.05), total nitrogen content (r=0.570, p＜0.01), and available phosphorus content (r=0.311, p＜0.05) were closely related to the soil ARG subtypes; the dissolved organic carbon (DOC) content (r=0.275, p＜0.05) and available potassium content (r=0.312, p＜0.05) were correlated with the ARG subtypes, and the DOC content (r=0.201, p＜0.05) significantly affected the pathogen community structure in the rice stubble. In summary, straw burning can reduce the relative abundance of X. oryzae in the rice stubble, which suggests that it may be a viable option for preventing and controlling rice bacterial leaf blight; moreover, a reduction in the positive correlation ratio of pathogens-ARGs and in the relative abundance of MGEs after straw burning may inhibit the formation of potentially resistant pathogens. However, given the complexity of soil biological compound pollution, the effects of straw burning on pathogens and ARGs in paddy fields need to be further explored by long-term location experiments. |
| format | Article |
| id | doaj-art-a0217dc63020440b8b3e8a7888ee70db |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2023-12-01 |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-a0217dc63020440b8b3e8a7888ee70db2025-08-20T02:47:32ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552023-12-014984085210.3785/j.issn.1008-9209.2023.01.13110089209Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blightWANG BinhaoCHEN YanboLIU RongjieWANG DongqunDONG ZuozhenDI RuiWANG XiaoWU YupingBacterial leaf blight in rice is a significant disease, and understanding the effects of straw burning on pathogens and antibiotic resistance genes (ARGs) in paddy fields is crucial. Soil and rice stubble samples were collected from bacterial leaf blight outbreak areas in Ningbo City of Zhejiang Province, and these samples were subjected to high-throughput metagenomic sequencing to investigate the effects of straw burning. The results indicated that the relative abundance of pathogens in the rice stubble was significantly higher than that in the soil. After straw burning, except for the significant increase in available potassium content, there were no significant changes in the other physicochemical properties of the soil. Overall, there were no significant differences in pathogen community diversity after straw burning, but straw burning led to a sharp decrease in the relative abundance of Xanthomonas oryzae in the rice stubble. The relative abundances of ARGs in the soil and rice stubble increased after straw burning, but there were no significant differences compared with those before straw burning (P＞0.05), while the relative abundance of mobile genetic elements (MGEs) in the rice stubble decreased significantly after straw burning (P＜0.05). The proportion of positively correlated links in the coexisting networks of pathogens-ARGs decreased after straw burning. Mantel analysis revealed that the water content (r=0.642, p＜0.01), pH value (r=0.582, p＜0.05), total organic carbon content (r=0.325, p＜0.05), total nitrogen content (r=0.570, p＜0.01), and available phosphorus content (r=0.311, p＜0.05) were closely related to the soil ARG subtypes; the dissolved organic carbon (DOC) content (r=0.275, p＜0.05) and available potassium content (r=0.312, p＜0.05) were correlated with the ARG subtypes, and the DOC content (r=0.201, p＜0.05) significantly affected the pathogen community structure in the rice stubble. In summary, straw burning can reduce the relative abundance of X. oryzae in the rice stubble, which suggests that it may be a viable option for preventing and controlling rice bacterial leaf blight; moreover, a reduction in the positive correlation ratio of pathogens-ARGs and in the relative abundance of MGEs after straw burning may inhibit the formation of potentially resistant pathogens. However, given the complexity of soil biological compound pollution, the effects of straw burning on pathogens and ARGs in paddy fields need to be further explored by long-term location experiments.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.01.131straw burningbacterial leaf blightpathogensantibiotic resistance genes |
| spellingShingle | WANG Binhao CHEN Yanbo LIU Rongjie WANG Dongqun DONG Zuozhen DI Rui WANG Xiao WU Yuping Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight 浙江大学学报. 农业与生命科学版 straw burning bacterial leaf blight pathogens antibiotic resistance genes |
| title | Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight |
| title_full | Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight |
| title_fullStr | Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight |
| title_full_unstemmed | Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight |
| title_short | Effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight |
| title_sort | effects of straw burning on pathogens and antibiotic resistance genes in paddy fields with bacterial leaf blight |
| topic | straw burning bacterial leaf blight pathogens antibiotic resistance genes |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.01.131 |
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