Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells
The aim of this study was to elucidate the molecular status of single human adult germ stem cells (haGSCs) and haGSC colonies, which spontaneously developed from the CD49f MACS and matrix- (collagen−/laminin+ binding-) selected fraction of enriched spermatogonia. Single-cell transcriptional profilin...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/8582526 |
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| author | Sabine Conrad Hossein Azizi Maryam Hatami Mikael Kubista Michael Bonin Jörg Hennenlotter Karl-Dietrich Sievert Thomas Skutella |
| author_facet | Sabine Conrad Hossein Azizi Maryam Hatami Mikael Kubista Michael Bonin Jörg Hennenlotter Karl-Dietrich Sievert Thomas Skutella |
| author_sort | Sabine Conrad |
| collection | DOAJ |
| description | The aim of this study was to elucidate the molecular status of single human adult germ stem cells (haGSCs) and haGSC colonies, which spontaneously developed from the CD49f MACS and matrix- (collagen−/laminin+ binding-) selected fraction of enriched spermatogonia. Single-cell transcriptional profiling by Fluidigm BioMark system of a long-term cultured haGSCs cluster in comparison to human embryonic stem cells (hESCs) and human fibroblasts (hFibs) revealed that haGSCs showed a characteristic germ- and pluripotency-associated gene expression profile with some similarities to hESCs and with a significant distinction from somatic hFibs. Genome-wide comparisons with microarray analysis confirmed that different haGSC colonies exhibited gene expression heterogeneity with more or less pluripotency. The results of this study confirm that haGSCs are adult stem cells with a specific molecular gene expression profile in vitro, related but not identical to true pluripotent stem cells. Under ES-cell conditions haGSC colonies could be selected and maintained in a partial pluripotent state at the molecular level, which may be related to their cell plasticity and potential to differentiate into cells of all germ layers. |
| format | Article |
| id | doaj-art-a0202e78d66c40f89cfdde438b859616 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a0202e78d66c40f89cfdde438b8596162025-08-20T02:20:51ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/85825268582526Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem CellsSabine Conrad0Hossein Azizi1Maryam Hatami2Mikael Kubista3Michael Bonin4Jörg Hennenlotter5Karl-Dietrich Sievert6Thomas Skutella7Sabine Conrad, P.O. Box 12 43, 72072 Tübingen, GermanyInstitute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, GermanyInstitute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, GermanyTATAA Biocenter AB, Odinsgatan 28, 41103 Göteborg, SwedenInstitute of Anthropology and Human Genetics, Microarray Facility, University Clinic, Calwerstraße 7, 72076 Tübingen, GermanyDepartment of Urology, University of Tübingen Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyDepartment of Urology, University of Tübingen Hospital, Hoppe-Seyler-Straße 3, 72076 Tübingen, GermanyInstitute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, GermanyThe aim of this study was to elucidate the molecular status of single human adult germ stem cells (haGSCs) and haGSC colonies, which spontaneously developed from the CD49f MACS and matrix- (collagen−/laminin+ binding-) selected fraction of enriched spermatogonia. Single-cell transcriptional profiling by Fluidigm BioMark system of a long-term cultured haGSCs cluster in comparison to human embryonic stem cells (hESCs) and human fibroblasts (hFibs) revealed that haGSCs showed a characteristic germ- and pluripotency-associated gene expression profile with some similarities to hESCs and with a significant distinction from somatic hFibs. Genome-wide comparisons with microarray analysis confirmed that different haGSC colonies exhibited gene expression heterogeneity with more or less pluripotency. The results of this study confirm that haGSCs are adult stem cells with a specific molecular gene expression profile in vitro, related but not identical to true pluripotent stem cells. Under ES-cell conditions haGSC colonies could be selected and maintained in a partial pluripotent state at the molecular level, which may be related to their cell plasticity and potential to differentiate into cells of all germ layers.http://dx.doi.org/10.1155/2016/8582526 |
| spellingShingle | Sabine Conrad Hossein Azizi Maryam Hatami Mikael Kubista Michael Bonin Jörg Hennenlotter Karl-Dietrich Sievert Thomas Skutella Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells Stem Cells International |
| title | Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells |
| title_full | Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells |
| title_fullStr | Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells |
| title_full_unstemmed | Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells |
| title_short | Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells |
| title_sort | expression of genes related to germ cell lineage and pluripotency in single cells and colonies of human adult germ stem cells |
| url | http://dx.doi.org/10.1155/2016/8582526 |
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