Innovative determination of phytohormones in Aloe vera
IntroductionAloe vera is widely known for its therapeutic properties, but concerns regarding the levels of phytohormones and their potential impact on human health highlight the need for advanced analytical techniques. This study aims to develop and validate a sensitive method for the determination...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2024.1490639/full |
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| author | Muhammad K. Hakeem Meera Maraqa Sampath K. Elangovan Sampath K. Elangovan Esam Eldin Saeed Ajay Kumar Mishra Khaled M. Hazzouri Iltaf Shah Iltaf Shah Khaled M. A. Amiri |
| author_facet | Muhammad K. Hakeem Meera Maraqa Sampath K. Elangovan Sampath K. Elangovan Esam Eldin Saeed Ajay Kumar Mishra Khaled M. Hazzouri Iltaf Shah Iltaf Shah Khaled M. A. Amiri |
| author_sort | Muhammad K. Hakeem |
| collection | DOAJ |
| description | IntroductionAloe vera is widely known for its therapeutic properties, but concerns regarding the levels of phytohormones and their potential impact on human health highlight the need for advanced analytical techniques. This study aims to develop and validate a sensitive method for the determination of six key phytohormones in Aloe vera using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).MethodsA validated LC-MS/MS method was optimized for the determination and quantification of six phytohormones in Aloe vera: Abscisic Acid (ABA), Salicylic Acid (SA), Indole-3-Acetic Acid (I3AA), Gibberellic Acid (GA), 6-Benzylaminopurine (6BAP), and Isopentenyladenine (ISA). The sample extraction process and mobile phase composition were optimized to enhance chromatographic separation and mass spectrometry sensitivity. A C-18 column was used for separation, and a triple quadrupole mass spectrometer was employed for quantification. The method’s performance was assessed in terms of linearity, sensitivity, and limits of detection.ResultsThe LC-MS/MS method exhibited excellent linearity (R2 > 0.99) and low limits of detection for all six phytohormones. Four of the six analytes were identified as predominant in Aloe vera. Quantitative analysis showed that ABA was the most abundant phytohormone, with a median concentration of 8.39 ng/mL, followed by I3AA (4.32 ng/mL), SA (3.16 ng/mL), and GA (1.55 ng/mL).DiscussionThis study provides a comprehensive and validated LC-MS/MS method for profiling phytohormones in Aloe vera. The results underscore the significant role of ABA, I3AA, SA, and GA in the plant's hormonal profile, offering a valuable tool for the analysis of phytohormonal content in Aloe vera and other plant species. The method is particularly beneficial for addressing health-related concerns regarding the presence and concentration of phytohormones in Aloe vera. |
| format | Article |
| id | doaj-art-a01ca7f1d4e9470f98ae5635c61fbad3 |
| institution | Kabale University |
| issn | 2296-2646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Chemistry |
| spelling | doaj-art-a01ca7f1d4e9470f98ae5635c61fbad32025-01-20T11:36:32ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462025-01-011210.3389/fchem.2024.14906391490639Innovative determination of phytohormones in Aloe veraMuhammad K. Hakeem0Meera Maraqa1Sampath K. Elangovan2Sampath K. Elangovan3Esam Eldin Saeed4Ajay Kumar Mishra5Khaled M. Hazzouri6Iltaf Shah7Iltaf Shah8Khaled M. A. Amiri9Department of Chemistry, College of Science, United Arab Emirates University (UAEU), Al Ain, United Arab EmiratesDepartment of Chemistry, College of Science, United Arab Emirates University (UAEU), Al Ain, United Arab EmiratesDepartment of Chemistry, College of Science, United Arab Emirates University (UAEU), Al Ain, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Chemistry, College of Science, United Arab Emirates University (UAEU), Al Ain, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, United Arab EmiratesIntroductionAloe vera is widely known for its therapeutic properties, but concerns regarding the levels of phytohormones and their potential impact on human health highlight the need for advanced analytical techniques. This study aims to develop and validate a sensitive method for the determination of six key phytohormones in Aloe vera using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).MethodsA validated LC-MS/MS method was optimized for the determination and quantification of six phytohormones in Aloe vera: Abscisic Acid (ABA), Salicylic Acid (SA), Indole-3-Acetic Acid (I3AA), Gibberellic Acid (GA), 6-Benzylaminopurine (6BAP), and Isopentenyladenine (ISA). The sample extraction process and mobile phase composition were optimized to enhance chromatographic separation and mass spectrometry sensitivity. A C-18 column was used for separation, and a triple quadrupole mass spectrometer was employed for quantification. The method’s performance was assessed in terms of linearity, sensitivity, and limits of detection.ResultsThe LC-MS/MS method exhibited excellent linearity (R2 > 0.99) and low limits of detection for all six phytohormones. Four of the six analytes were identified as predominant in Aloe vera. Quantitative analysis showed that ABA was the most abundant phytohormone, with a median concentration of 8.39 ng/mL, followed by I3AA (4.32 ng/mL), SA (3.16 ng/mL), and GA (1.55 ng/mL).DiscussionThis study provides a comprehensive and validated LC-MS/MS method for profiling phytohormones in Aloe vera. The results underscore the significant role of ABA, I3AA, SA, and GA in the plant's hormonal profile, offering a valuable tool for the analysis of phytohormonal content in Aloe vera and other plant species. The method is particularly beneficial for addressing health-related concerns regarding the presence and concentration of phytohormones in Aloe vera.https://www.frontiersin.org/articles/10.3389/fchem.2024.1490639/fullLC-MS/MSAloe veraphytohormonesliquid chromatography mass spectrometryplant hormonemethod validation |
| spellingShingle | Muhammad K. Hakeem Meera Maraqa Sampath K. Elangovan Sampath K. Elangovan Esam Eldin Saeed Ajay Kumar Mishra Khaled M. Hazzouri Iltaf Shah Iltaf Shah Khaled M. A. Amiri Innovative determination of phytohormones in Aloe vera Frontiers in Chemistry LC-MS/MS Aloe vera phytohormones liquid chromatography mass spectrometry plant hormone method validation |
| title | Innovative determination of phytohormones in Aloe vera |
| title_full | Innovative determination of phytohormones in Aloe vera |
| title_fullStr | Innovative determination of phytohormones in Aloe vera |
| title_full_unstemmed | Innovative determination of phytohormones in Aloe vera |
| title_short | Innovative determination of phytohormones in Aloe vera |
| title_sort | innovative determination of phytohormones in aloe vera |
| topic | LC-MS/MS Aloe vera phytohormones liquid chromatography mass spectrometry plant hormone method validation |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2024.1490639/full |
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