Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production
ABSTRACT Xylose is a common monosaccharide in lignocellulosic residues that Yarrowia lipolytica cannot naturally metabolise for lipid production and therefore, heterologous xylose metabolic pathways must be engineered in this yeast to facilitate its consumption. We have compared the metabolic effici...
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Wiley
2025-03-01
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| Series: | Microbial Biotechnology |
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| Online Access: | https://doi.org/10.1111/1751-7915.70127 |
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| author | Isabel De La Torre Miguel G. Acedos Juan J. Cestero Jorge Barriuso José L. García |
| author_facet | Isabel De La Torre Miguel G. Acedos Juan J. Cestero Jorge Barriuso José L. García |
| author_sort | Isabel De La Torre |
| collection | DOAJ |
| description | ABSTRACT Xylose is a common monosaccharide in lignocellulosic residues that Yarrowia lipolytica cannot naturally metabolise for lipid production and therefore, heterologous xylose metabolic pathways must be engineered in this yeast to facilitate its consumption. We have compared the metabolic efficiency of two xylose metabolic pathways by developing three recombinant Y. lipolytica strains: one harbouring a xylose reductase pathway, one with a xylose isomerase pathway, and one combining both pathways, and the strains were tested for xylose consumption and lipid production at different scales. The recombinant strain with the reductase pathway that was directly isolated in selective xylose medium showed the highest lipid yield, producing up to 12.8 g/L of lipids, or 43% of the biomass dry weight, without requiring any other xylose consumption adaptive evolution process. This strain achieved a lipid yield of 0.13 g lipids/g xylose, one of the highest yields in yeast reported so far using xylose as the sole carbon and energy source. Although the strain harbouring the isomerase pathway performed better under oxygen‐limiting conditions and led to higher lipid intracellular accumulation, it showed a lower xylose uptake and biomass production, rendering a lower yield under non‐limiting oxygen conditions. Unexpectedly, the combination of both pathways in the same strain was less effective than the use of the reductase pathway alone. |
| format | Article |
| id | doaj-art-a01b50ebe6894429a8890a0cbe60ff4a |
| institution | DOAJ |
| issn | 1751-7915 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
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| series | Microbial Biotechnology |
| spelling | doaj-art-a01b50ebe6894429a8890a0cbe60ff4a2025-08-20T02:42:34ZengWileyMicrobial Biotechnology1751-79152025-03-01183n/an/a10.1111/1751-7915.70127Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid ProductionIsabel De La Torre0Miguel G. Acedos1Juan J. Cestero2Jorge Barriuso3José L. García4Department of Biotechnology Centro de Investigaciones Biológicas Margarita Salas. Consejo Superior de Investigaciones Científicas (CSIC) Madrid SpainDepartment of Biotechnology Centro de Investigaciones Biológicas Margarita Salas. Consejo Superior de Investigaciones Científicas (CSIC) Madrid SpainDepartment of Biotechnology Centro de Investigaciones Biológicas Margarita Salas. Consejo Superior de Investigaciones Científicas (CSIC) Madrid SpainDepartment of Biotechnology Centro de Investigaciones Biológicas Margarita Salas. Consejo Superior de Investigaciones Científicas (CSIC) Madrid SpainDepartment of Biotechnology Centro de Investigaciones Biológicas Margarita Salas. Consejo Superior de Investigaciones Científicas (CSIC) Madrid SpainABSTRACT Xylose is a common monosaccharide in lignocellulosic residues that Yarrowia lipolytica cannot naturally metabolise for lipid production and therefore, heterologous xylose metabolic pathways must be engineered in this yeast to facilitate its consumption. We have compared the metabolic efficiency of two xylose metabolic pathways by developing three recombinant Y. lipolytica strains: one harbouring a xylose reductase pathway, one with a xylose isomerase pathway, and one combining both pathways, and the strains were tested for xylose consumption and lipid production at different scales. The recombinant strain with the reductase pathway that was directly isolated in selective xylose medium showed the highest lipid yield, producing up to 12.8 g/L of lipids, or 43% of the biomass dry weight, without requiring any other xylose consumption adaptive evolution process. This strain achieved a lipid yield of 0.13 g lipids/g xylose, one of the highest yields in yeast reported so far using xylose as the sole carbon and energy source. Although the strain harbouring the isomerase pathway performed better under oxygen‐limiting conditions and led to higher lipid intracellular accumulation, it showed a lower xylose uptake and biomass production, rendering a lower yield under non‐limiting oxygen conditions. Unexpectedly, the combination of both pathways in the same strain was less effective than the use of the reductase pathway alone.https://doi.org/10.1111/1751-7915.70127lignocelluloselipidsxyloseYarrowia lipolytica |
| spellingShingle | Isabel De La Torre Miguel G. Acedos Juan J. Cestero Jorge Barriuso José L. García Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production Microbial Biotechnology lignocellulose lipids xylose Yarrowia lipolytica |
| title | Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production |
| title_full | Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production |
| title_fullStr | Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production |
| title_full_unstemmed | Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production |
| title_short | Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production |
| title_sort | engineering xylose isomerase and reductase pathways in yarrowia lipolytica for efficient lipid production |
| topic | lignocellulose lipids xylose Yarrowia lipolytica |
| url | https://doi.org/10.1111/1751-7915.70127 |
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