Targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLD
Abstract Altered gut metabolites are important for the inflammatory progression in children with NAFLD. Fecal and plasma samples were collected from 145 subjects including 53 non-alcoholic fatty liver (NAFL), 39 nonalcoholic steatohepatitis (NASH) and 53 obese controls. We performed G350 targeted in...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-025-00706-w |
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| author | Miyang Luo Jiayou Luo Atipatsa C. Kaminga Jia Wei Wen Dai Yan Zhong Ningan Xu Xiongwei Li Haixiang Zhou Xiongfeng Pan |
| author_facet | Miyang Luo Jiayou Luo Atipatsa C. Kaminga Jia Wei Wen Dai Yan Zhong Ningan Xu Xiongwei Li Haixiang Zhou Xiongfeng Pan |
| author_sort | Miyang Luo |
| collection | DOAJ |
| description | Abstract Altered gut metabolites are important for the inflammatory progression in children with NAFLD. Fecal and plasma samples were collected from 145 subjects including 53 non-alcoholic fatty liver (NAFL), 39 nonalcoholic steatohepatitis (NASH) and 53 obese controls. We performed G350 targeted integrative metabolomics using high performance liquid chromatography mass spectrometry for fecal and plasma analysis of NAFL, NASH, and obese children. We found 9 metabolites involved in metabolic reprogramming of inflammation in NAFLD, such as lipid, carbohydrate, amino acid metabolism, and TCA cycle pathway. Moreover, 7 inflammation-related metabolites could discriminate NAFLD severity by machine learning model. This study identified three novel elevated inflammatory pathogenic metabolites and the relationship between increased inflammation, may be involved in TLR5/MYD88/NFκB pathway. These findings reveal that specific inflammatory metabolites entering the blood circulation from the gut are associated with disease severity and inflammatory pathogenesis in children with NAFLD. |
| format | Article |
| id | doaj-art-a00f16d5318d481a93aab9ddc608edb2 |
| institution | DOAJ |
| issn | 2055-5008 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-a00f16d5318d481a93aab9ddc608edb22025-08-20T03:03:38ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-07-0111111310.1038/s41522-025-00706-wTargeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLDMiyang Luo0Jiayou Luo1Atipatsa C. Kaminga2Jia Wei3Wen Dai4Yan Zhong5Ningan Xu6Xiongwei Li7Haixiang Zhou8Xiongfeng Pan9Department of Epidemiology and Health Statistics, Department of Maternal and Child Health, Xiangya School of Public Health, Central South UniversityDepartment of Epidemiology and Health Statistics, Department of Maternal and Child Health, Xiangya School of Public Health, Central South UniversityDepartment of Epidemiology and Health Statistics, Department of Maternal and Child Health, Xiangya School of Public Health, Central South UniversityDepartment of Epidemiology and Health Statistics, Department of Maternal and Child Health, Xiangya School of Public Health, Central South UniversityDepartment of Epidemiology and Health Statistics, Department of Maternal and Child Health, Xiangya School of Public Health, Central South UniversityInstitute of Children Health, Hunan Children’s HospitalInstitute of Children Health, Hunan Children’s HospitalCenter for Disease Control and Prevention of NingxiangCenter for Disease Control and Prevention of NingxiangPediatrics Research Institute of Hunan Province, Hunan Children’s Hospital, The Affiliated Children’s Hospital of Xiangya School of Medicine, Central South UniversityAbstract Altered gut metabolites are important for the inflammatory progression in children with NAFLD. Fecal and plasma samples were collected from 145 subjects including 53 non-alcoholic fatty liver (NAFL), 39 nonalcoholic steatohepatitis (NASH) and 53 obese controls. We performed G350 targeted integrative metabolomics using high performance liquid chromatography mass spectrometry for fecal and plasma analysis of NAFL, NASH, and obese children. We found 9 metabolites involved in metabolic reprogramming of inflammation in NAFLD, such as lipid, carbohydrate, amino acid metabolism, and TCA cycle pathway. Moreover, 7 inflammation-related metabolites could discriminate NAFLD severity by machine learning model. This study identified three novel elevated inflammatory pathogenic metabolites and the relationship between increased inflammation, may be involved in TLR5/MYD88/NFκB pathway. These findings reveal that specific inflammatory metabolites entering the blood circulation from the gut are associated with disease severity and inflammatory pathogenesis in children with NAFLD.https://doi.org/10.1038/s41522-025-00706-w |
| spellingShingle | Miyang Luo Jiayou Luo Atipatsa C. Kaminga Jia Wei Wen Dai Yan Zhong Ningan Xu Xiongwei Li Haixiang Zhou Xiongfeng Pan Targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLD npj Biofilms and Microbiomes |
| title | Targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLD |
| title_full | Targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLD |
| title_fullStr | Targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLD |
| title_full_unstemmed | Targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLD |
| title_short | Targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLD |
| title_sort | targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with nafld |
| url | https://doi.org/10.1038/s41522-025-00706-w |
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