Developing Immunoniosomes (INs): Antibody and Fab conjugations of niosomal nanoparticles via UV-NBS and EDC/NHS chemistry for treating glioblastoma cells
Antibody-conjugated nanoparticles (ACNPs), particularly immunoliposomes (ILs), have gained significant attention in cancer treatment due to their enhanced efficacy and superior tissue penetration. However, their high production costs and technical challenges underscore the need for more cost-effecti...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | International Journal of Pharmaceutics: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590156725000520 |
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| author | Nilufer Cakir Hatice Oncel Aylin Ozkan Dilan Bicak Sibel Akgun Bas Nur Mustafaoglu |
| author_facet | Nilufer Cakir Hatice Oncel Aylin Ozkan Dilan Bicak Sibel Akgun Bas Nur Mustafaoglu |
| author_sort | Nilufer Cakir |
| collection | DOAJ |
| description | Antibody-conjugated nanoparticles (ACNPs), particularly immunoliposomes (ILs), have gained significant attention in cancer treatment due to their enhanced efficacy and superior tissue penetration. However, their high production costs and technical challenges underscore the need for more cost-effective alternatives. Niosomes, with their lower production costs, improved stability, and biocompatibility, have emerged as promising alternatives to liposomes in drug delivery. This study introduces immunoniosomes (INs), a novel class of antibody-conjugated niosomes, through two conjugation strategies: (i) UV-NBS, a site-specific covalent conjugation method utilizing an indole ring structure for moderate binding to the variable regions of antibodies and Fab fragments, and (ii) EDC/NHS chemistry, which conjugates antibodies to carboxylated niosomes via primary amines on lysine sidechains. Bevacizumab, a monoclonal antibody targeting VEGF and approved for the treatment of various cancers including glioblastoma multiforme (GBM), was used as a model therapeutic. Both Bevacizumab and its Fab fragment were conjugated to niosomes and evaluated in U87 glioma cells (overexpressing VEGF) and human umbilical vein endothelial cells (HUVECs) (representing normal VEGF expression). Physicochemical characterization of the conjugated niosomes confirmed hydrodynamic sizes ranging from 100 to 200 nm, neutral surface charge, and dispersity indices below 0.5—properties critical for effective cellular penetration and drug delivery. Cellular toxicity assays, conducted at a 10× dilution from commonly reported concentrations, highlighted the role of the autocrine loop in U87 glioblastoma cells. Importantly, specific Nio-Fab conjugate formulations, created through both site-specific and randomized conjugation strategies, exhibited enhanced cytotoxicity toward U87 cells while sparing healthy endothelial HUVEC cells. In summary, this research establishes novel conjugation strategies to produce stable, site-specific, and randomized antibody-niosomal conjugates with enhanced half-life and selective toxicity against GBM cells. By offering an alternative route for antibody delivery through niosomal nanocarriers, these findings open new avenues for the development of more effective GBM therapeutics, warranting further non-clinical and clinical investigations. |
| format | Article |
| id | doaj-art-a00e97da36c44897a8612daeda837299 |
| institution | DOAJ |
| issn | 2590-1567 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Pharmaceutics: X |
| spelling | doaj-art-a00e97da36c44897a8612daeda8372992025-08-20T03:08:36ZengElsevierInternational Journal of Pharmaceutics: X2590-15672025-12-011010036710.1016/j.ijpx.2025.100367Developing Immunoniosomes (INs): Antibody and Fab conjugations of niosomal nanoparticles via UV-NBS and EDC/NHS chemistry for treating glioblastoma cellsNilufer Cakir0Hatice Oncel1Aylin Ozkan2Dilan Bicak3Sibel Akgun Bas4Nur Mustafaoglu5Faculty of Engineering and Natural Sciences, Sabanci University, Tuzla 34596, Istanbul, Turkiye; ILKO, Sancaktepe 34885, Istanbul, Turkiye; Sabanci University Nanotechnology Research and Application Center (SUNUM), Tuzla 34596, Istanbul, TurkiyeILKO, Sancaktepe 34885, Istanbul, TurkiyeILKO, Sancaktepe 34885, Istanbul, TurkiyeILKO, Sancaktepe 34885, Istanbul, TurkiyeILKO, Sancaktepe 34885, Istanbul, TurkiyeFaculty of Engineering and Natural Sciences, Sabanci University, Tuzla 34596, Istanbul, Turkiye; Sabanci University Nanotechnology Research and Application Center (SUNUM), Tuzla 34596, Istanbul, Turkiye; Corresponding author at: Faculty of Engineering and Natural Sciences, Sabanci University, Tuzla 34596, Istanbul, Turkiye.Antibody-conjugated nanoparticles (ACNPs), particularly immunoliposomes (ILs), have gained significant attention in cancer treatment due to their enhanced efficacy and superior tissue penetration. However, their high production costs and technical challenges underscore the need for more cost-effective alternatives. Niosomes, with their lower production costs, improved stability, and biocompatibility, have emerged as promising alternatives to liposomes in drug delivery. This study introduces immunoniosomes (INs), a novel class of antibody-conjugated niosomes, through two conjugation strategies: (i) UV-NBS, a site-specific covalent conjugation method utilizing an indole ring structure for moderate binding to the variable regions of antibodies and Fab fragments, and (ii) EDC/NHS chemistry, which conjugates antibodies to carboxylated niosomes via primary amines on lysine sidechains. Bevacizumab, a monoclonal antibody targeting VEGF and approved for the treatment of various cancers including glioblastoma multiforme (GBM), was used as a model therapeutic. Both Bevacizumab and its Fab fragment were conjugated to niosomes and evaluated in U87 glioma cells (overexpressing VEGF) and human umbilical vein endothelial cells (HUVECs) (representing normal VEGF expression). Physicochemical characterization of the conjugated niosomes confirmed hydrodynamic sizes ranging from 100 to 200 nm, neutral surface charge, and dispersity indices below 0.5—properties critical for effective cellular penetration and drug delivery. Cellular toxicity assays, conducted at a 10× dilution from commonly reported concentrations, highlighted the role of the autocrine loop in U87 glioblastoma cells. Importantly, specific Nio-Fab conjugate formulations, created through both site-specific and randomized conjugation strategies, exhibited enhanced cytotoxicity toward U87 cells while sparing healthy endothelial HUVEC cells. In summary, this research establishes novel conjugation strategies to produce stable, site-specific, and randomized antibody-niosomal conjugates with enhanced half-life and selective toxicity against GBM cells. By offering an alternative route for antibody delivery through niosomal nanocarriers, these findings open new avenues for the development of more effective GBM therapeutics, warranting further non-clinical and clinical investigations.http://www.sciencedirect.com/science/article/pii/S2590156725000520Antibody-conjugated nanoparticlesBevacizumabGlioblastoma multiformeNiosomeSite-specific conjugationUV-NBS |
| spellingShingle | Nilufer Cakir Hatice Oncel Aylin Ozkan Dilan Bicak Sibel Akgun Bas Nur Mustafaoglu Developing Immunoniosomes (INs): Antibody and Fab conjugations of niosomal nanoparticles via UV-NBS and EDC/NHS chemistry for treating glioblastoma cells International Journal of Pharmaceutics: X Antibody-conjugated nanoparticles Bevacizumab Glioblastoma multiforme Niosome Site-specific conjugation UV-NBS |
| title | Developing Immunoniosomes (INs): Antibody and Fab conjugations of niosomal nanoparticles via UV-NBS and EDC/NHS chemistry for treating glioblastoma cells |
| title_full | Developing Immunoniosomes (INs): Antibody and Fab conjugations of niosomal nanoparticles via UV-NBS and EDC/NHS chemistry for treating glioblastoma cells |
| title_fullStr | Developing Immunoniosomes (INs): Antibody and Fab conjugations of niosomal nanoparticles via UV-NBS and EDC/NHS chemistry for treating glioblastoma cells |
| title_full_unstemmed | Developing Immunoniosomes (INs): Antibody and Fab conjugations of niosomal nanoparticles via UV-NBS and EDC/NHS chemistry for treating glioblastoma cells |
| title_short | Developing Immunoniosomes (INs): Antibody and Fab conjugations of niosomal nanoparticles via UV-NBS and EDC/NHS chemistry for treating glioblastoma cells |
| title_sort | developing immunoniosomes ins antibody and fab conjugations of niosomal nanoparticles via uv nbs and edc nhs chemistry for treating glioblastoma cells |
| topic | Antibody-conjugated nanoparticles Bevacizumab Glioblastoma multiforme Niosome Site-specific conjugation UV-NBS |
| url | http://www.sciencedirect.com/science/article/pii/S2590156725000520 |
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