HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells
Summary: Herpes simplex virus type 1 (HSV-1) significantly impairs dendritic cell (DC) function, ultimately eliciting the death of these cells. Here, we sought to assess whether HSV-1 modulates lipid metabolism in mouse DCs as a mechanism of immune evasion. For this, we performed RT-qPCR gene arrays...
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| Language: | English |
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Elsevier
2025-05-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225007023 |
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| author | Mónica A. Farías Felipe A. Cancino Areli J. Navarro Luisa F. Duarte Abel A. Soto Eduardo I. Tognarelli Maximiliano J. Ramm Bárbara N. Alarcón-Zapata José Cordero Sergio San Martín Cristian Agurto-Muñoz Angello Retamal-Díaz Claudia A. Riedel Nelson P. Barrera Luis Bustamante Susan M. Bueno Alexis M. Kalergis Pablo A. González |
| author_facet | Mónica A. Farías Felipe A. Cancino Areli J. Navarro Luisa F. Duarte Abel A. Soto Eduardo I. Tognarelli Maximiliano J. Ramm Bárbara N. Alarcón-Zapata José Cordero Sergio San Martín Cristian Agurto-Muñoz Angello Retamal-Díaz Claudia A. Riedel Nelson P. Barrera Luis Bustamante Susan M. Bueno Alexis M. Kalergis Pablo A. González |
| author_sort | Mónica A. Farías |
| collection | DOAJ |
| description | Summary: Herpes simplex virus type 1 (HSV-1) significantly impairs dendritic cell (DC) function, ultimately eliciting the death of these cells. Here, we sought to assess whether HSV-1 modulates lipid metabolism in mouse DCs as a mechanism of immune evasion. For this, we performed RT-qPCR gene arrays with ingenuity pathway analysis (IPA), RNA sequencing (RNA-seq) and gene set enrichment analysis (GSEA), confocal microscopy, transmission electron microscopy, ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) analysis, pharmacological inhibition of eight lipid-metabolism-related enzymes in HSV-1-infected DCs, co-cultures between virus-specific transgenic CD4+ and CD8+ T cells and HSV-1-infected DCs, and in vivo assays with mice. We found that HSV-1 significantly alters lipid metabolism in DCs and induces lipid droplet (LD) accumulation in these cells. Pharmacological inhibition of two particular lipid metabolism enzymes was found to partially restore DC function. Overall, these results suggest that lipid metabolism plays an important role in the impairment of DC function by HSV-1. |
| format | Article |
| id | doaj-art-a0075b9b76224c2390bbe5366d4a69c9 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-a0075b9b76224c2390bbe5366d4a69c92025-08-20T03:51:59ZengElsevieriScience2589-00422025-05-0128511244110.1016/j.isci.2025.112441HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cellsMónica A. Farías0Felipe A. Cancino1Areli J. Navarro2Luisa F. Duarte3Abel A. Soto4Eduardo I. Tognarelli5Maximiliano J. Ramm6Bárbara N. Alarcón-Zapata7José Cordero8Sergio San Martín9Cristian Agurto-Muñoz10Angello Retamal-Díaz11Claudia A. Riedel12Nelson P. Barrera13Luis Bustamante14Susan M. Bueno15Alexis M. Kalergis16Pablo A. González17Millennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileDepartamento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Concepción, ChileDepartamento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Concepción, ChileFacultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileGrupo Interdisciplinario de Biotecnología Marina (GIBMAR), Centro de Biotecnología, Universidad de Concepción, Concepción, ChileGrupo Interdisciplinario de Biotecnología Marina (GIBMAR), Centro de Biotecnología, Universidad de Concepción, Concepción, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Departamento de Biotecnología, Facultad de Ciencias del Mar y de Recursos Biológicos, Universidad de Antofagasta, Antofagasta, Chile; Centro de Investigación en Inmunología y Biotecnología Biomédica de Antofagasta, Hospital Clínico Universidad de Antofagasta, Antofagasta, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Centro de Investigación para la Resiliencia a Pandemias, Facultad Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileFacultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileDepartamento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Concepción, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile; Departamento de Endocrinología, Facultad de Medicina, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, ChileMillennium Institute on Immunology and Immunotherapy, Chile; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile; Corresponding authorSummary: Herpes simplex virus type 1 (HSV-1) significantly impairs dendritic cell (DC) function, ultimately eliciting the death of these cells. Here, we sought to assess whether HSV-1 modulates lipid metabolism in mouse DCs as a mechanism of immune evasion. For this, we performed RT-qPCR gene arrays with ingenuity pathway analysis (IPA), RNA sequencing (RNA-seq) and gene set enrichment analysis (GSEA), confocal microscopy, transmission electron microscopy, ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) analysis, pharmacological inhibition of eight lipid-metabolism-related enzymes in HSV-1-infected DCs, co-cultures between virus-specific transgenic CD4+ and CD8+ T cells and HSV-1-infected DCs, and in vivo assays with mice. We found that HSV-1 significantly alters lipid metabolism in DCs and induces lipid droplet (LD) accumulation in these cells. Pharmacological inhibition of two particular lipid metabolism enzymes was found to partially restore DC function. Overall, these results suggest that lipid metabolism plays an important role in the impairment of DC function by HSV-1.http://www.sciencedirect.com/science/article/pii/S2589004225007023Natural sciencesBiological sciencesBiochemistryImmunologyMicrobiologyCell biology |
| spellingShingle | Mónica A. Farías Felipe A. Cancino Areli J. Navarro Luisa F. Duarte Abel A. Soto Eduardo I. Tognarelli Maximiliano J. Ramm Bárbara N. Alarcón-Zapata José Cordero Sergio San Martín Cristian Agurto-Muñoz Angello Retamal-Díaz Claudia A. Riedel Nelson P. Barrera Luis Bustamante Susan M. Bueno Alexis M. Kalergis Pablo A. González HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells iScience Natural sciences Biological sciences Biochemistry Immunology Microbiology Cell biology |
| title | HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells |
| title_full | HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells |
| title_fullStr | HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells |
| title_full_unstemmed | HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells |
| title_short | HSV-1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells |
| title_sort | hsv 1 alters lipid metabolism and induces lipid droplet accumulation in functionally impaired mouse dendritic cells |
| topic | Natural sciences Biological sciences Biochemistry Immunology Microbiology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225007023 |
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