5‐HT6 receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia

Abstract Cognitive deficits in schizophrenia severely compromise quality of life and are poorly controlled by current antipsychotics. While 5‐HT6 receptor blockade holds special promise, molecular substrates underlying their control of cognition remain unclear. Using a proteomic strategy, we show th...

Full description

Saved in:
Bibliographic Details
Main Authors: Julie Meffre, Séverine Chaumont‐Dubel, Clotilde Mannoury la Cour, Florence Loiseau, David J. G. Watson, Anne Dekeyne, Martial Séveno, Jean‐Michel Rivet, Florence Gaven, Paul Déléris, Denis Hervé, Kevin C. F. Fone, Joël Bockaert, Mark J. Millan, Philippe Marin
Format: Article
Language:English
Published: Springer Nature 2012-10-01
Series:EMBO Molecular Medicine
Subjects:
Online Access:https://doi.org/10.1002/emmm.201201410
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850179082193469440
author Julie Meffre
Séverine Chaumont‐Dubel
Clotilde Mannoury la Cour
Florence Loiseau
David J. G. Watson
Anne Dekeyne
Martial Séveno
Jean‐Michel Rivet
Florence Gaven
Paul Déléris
Denis Hervé
Kevin C. F. Fone
Joël Bockaert
Mark J. Millan
Philippe Marin
author_facet Julie Meffre
Séverine Chaumont‐Dubel
Clotilde Mannoury la Cour
Florence Loiseau
David J. G. Watson
Anne Dekeyne
Martial Séveno
Jean‐Michel Rivet
Florence Gaven
Paul Déléris
Denis Hervé
Kevin C. F. Fone
Joël Bockaert
Mark J. Millan
Philippe Marin
author_sort Julie Meffre
collection DOAJ
description Abstract Cognitive deficits in schizophrenia severely compromise quality of life and are poorly controlled by current antipsychotics. While 5‐HT6 receptor blockade holds special promise, molecular substrates underlying their control of cognition remain unclear. Using a proteomic strategy, we show that 5‐HT6 receptors physically interact with several proteins of the mammalian target of rapamycin (mTOR) pathway, including mTOR. Further, 5‐HT6 receptor activation increased mTOR signalling in rodent prefrontal cortex (PFC). Linking this signalling event to cognitive impairment, the mTOR inhibitor rapamycin prevented deficits in social cognition and novel object discrimination induced by 5‐HT6 agonists. In two developmental models of schizophrenia, specifically neonatal phencyclidine treatment and post‐weaning isolation rearing, the activity of mTOR was enhanced in the PFC, and rapamycin, like 5‐HT6 antagonists, reversed these cognitive deficits. These observations suggest that recruitment of mTOR by prefrontal 5‐HT6 receptors contributes to the perturbed cognition in schizophrenia, offering new vistas for its therapeutic control.
format Article
id doaj-art-a0066cfd997d434495adbb9a5a7bd59e
institution OA Journals
issn 1757-4676
1757-4684
language English
publishDate 2012-10-01
publisher Springer Nature
record_format Article
series EMBO Molecular Medicine
spelling doaj-art-a0066cfd997d434495adbb9a5a7bd59e2025-08-20T02:18:35ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842012-10-014101043105610.1002/emmm.2012014105‐HT6 receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophreniaJulie Meffre0Séverine Chaumont‐Dubel1Clotilde Mannoury la Cour2Florence Loiseau3David J. G. Watson4Anne Dekeyne5Martial Séveno6Jean‐Michel Rivet7Florence Gaven8Paul Déléris9Denis Hervé10Kevin C. F. Fone11Joël Bockaert12Mark J. Millan13Philippe Marin14CNRS, UMR‐5203, Institut de Génomique FonctionnelleCNRS, UMR‐5203, Institut de Génomique FonctionnelleInstitut de Recherches ServierInstitut de Recherches ServierSchool of Biomedical Sciences, Medical School, Queen's Medical Centre, The University of NottinghamInstitut de Recherches ServierCNRS, UMR‐5203, Institut de Génomique FonctionnelleInstitut de Recherches ServierCNRS, UMR‐5203, Institut de Génomique FonctionnelleCNRS, UMR‐5203, Institut de Génomique FonctionnelleInstitut du Fer à Moulin, INSERM, UMRS‐839, UPMCSchool of Biomedical Sciences, Medical School, Queen's Medical Centre, The University of NottinghamCNRS, UMR‐5203, Institut de Génomique FonctionnelleInstitut de Recherches ServierCNRS, UMR‐5203, Institut de Génomique FonctionnelleAbstract Cognitive deficits in schizophrenia severely compromise quality of life and are poorly controlled by current antipsychotics. While 5‐HT6 receptor blockade holds special promise, molecular substrates underlying their control of cognition remain unclear. Using a proteomic strategy, we show that 5‐HT6 receptors physically interact with several proteins of the mammalian target of rapamycin (mTOR) pathway, including mTOR. Further, 5‐HT6 receptor activation increased mTOR signalling in rodent prefrontal cortex (PFC). Linking this signalling event to cognitive impairment, the mTOR inhibitor rapamycin prevented deficits in social cognition and novel object discrimination induced by 5‐HT6 agonists. In two developmental models of schizophrenia, specifically neonatal phencyclidine treatment and post‐weaning isolation rearing, the activity of mTOR was enhanced in the PFC, and rapamycin, like 5‐HT6 antagonists, reversed these cognitive deficits. These observations suggest that recruitment of mTOR by prefrontal 5‐HT6 receptors contributes to the perturbed cognition in schizophrenia, offering new vistas for its therapeutic control.https://doi.org/10.1002/emmm.2012014105‐HT6 receptorcognitionmTORC1proteomicsschizophrenia
spellingShingle Julie Meffre
Séverine Chaumont‐Dubel
Clotilde Mannoury la Cour
Florence Loiseau
David J. G. Watson
Anne Dekeyne
Martial Séveno
Jean‐Michel Rivet
Florence Gaven
Paul Déléris
Denis Hervé
Kevin C. F. Fone
Joël Bockaert
Mark J. Millan
Philippe Marin
5‐HT6 receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia
EMBO Molecular Medicine
5‐HT6 receptor
cognition
mTORC1
proteomics
schizophrenia
title 5‐HT6 receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia
title_full 5‐HT6 receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia
title_fullStr 5‐HT6 receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia
title_full_unstemmed 5‐HT6 receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia
title_short 5‐HT6 receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia
title_sort 5 ht6 receptor recruitment of mtor as a mechanism for perturbed cognition in schizophrenia
topic 5‐HT6 receptor
cognition
mTORC1
proteomics
schizophrenia
url https://doi.org/10.1002/emmm.201201410
work_keys_str_mv AT juliemeffre 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT severinechaumontdubel 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT clotildemannourylacour 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT florenceloiseau 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT davidjgwatson 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT annedekeyne 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT martialseveno 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT jeanmichelrivet 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT florencegaven 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT pauldeleris 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT denisherve 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT kevincffone 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT joelbockaert 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT markjmillan 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia
AT philippemarin 5ht6receptorrecruitmentofmtorasamechanismforperturbedcognitioninschizophrenia