Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss
In East Africa, passion fruit woodiness disease is caused by potyviruses, among which are Cowpea Aphid-Borne Mosaic Virus (CABMV) and Uganda Passiflora Virus (UPV). Previous studies suggest that synergistic interaction of viruses causes mild or severe outcomes of the disease. However, mixed infectio...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Advances in Agriculture |
| Online Access: | http://dx.doi.org/10.1155/2020/8876498 |
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| author | Lucy J. Kiptui Faith J. Toroitich Dora C. Kilalo Meshack Obonyo |
| author_facet | Lucy J. Kiptui Faith J. Toroitich Dora C. Kilalo Meshack Obonyo |
| author_sort | Lucy J. Kiptui |
| collection | DOAJ |
| description | In East Africa, passion fruit woodiness disease is caused by potyviruses, among which are Cowpea Aphid-Borne Mosaic Virus (CABMV) and Uganda Passiflora Virus (UPV). Previous studies suggest that synergistic interaction of viruses causes mild or severe outcomes of the disease. However, mixed infections of these viruses have not been documented. The aim of this study was to determine the effects of the interaction between two CABMV isolates causing passion fruit woodiness disease in Kenya. Healthy plants were mechanically inoculated at six-leaf stage. Four treatments were used: CABMV isolate 1, CABMV isolate 2, mixture of the two isolates, and noninoculated control. The test plants were maintained in a screen house, and data on symptom severity and rate of disease development were recorded. ELISA test was conducted to confirm virus presence and distribution. The results indicate that the CABMV isolates used are systemic. There were various differences in disease progression, which was faster in the sweet passion variety than in the purple variety. However, the latter had a higher disease severity. Coinfected plants expressed severe symptoms compared to the singly infected ones, indicating synergistic interaction between the viral isolates. This implies that coinfection has adverse impact resulting in higher economic losses. |
| format | Article |
| id | doaj-art-9fffc14074d74cc5bf77fe5455a8d7b6 |
| institution | Kabale University |
| issn | 2356-654X 2314-7539 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Agriculture |
| spelling | doaj-art-9fffc14074d74cc5bf77fe5455a8d7b62025-08-20T03:38:27ZengWileyAdvances in Agriculture2356-654X2314-75392020-01-01202010.1155/2020/88764988876498Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis JussLucy J. Kiptui0Faith J. Toroitich1Dora C. Kilalo2Meshack Obonyo3Department of Biological Sciences, Egerton University, Njoro, KenyaDepartment of Biological Sciences, Egerton University, Njoro, KenyaDepartment of Plant Science and Crop Protection, University of Nairobi, Nairobi, KenyaDepartment of Biochemistry and Molecular Biology, Egerton University, Njoro, KenyaIn East Africa, passion fruit woodiness disease is caused by potyviruses, among which are Cowpea Aphid-Borne Mosaic Virus (CABMV) and Uganda Passiflora Virus (UPV). Previous studies suggest that synergistic interaction of viruses causes mild or severe outcomes of the disease. However, mixed infections of these viruses have not been documented. The aim of this study was to determine the effects of the interaction between two CABMV isolates causing passion fruit woodiness disease in Kenya. Healthy plants were mechanically inoculated at six-leaf stage. Four treatments were used: CABMV isolate 1, CABMV isolate 2, mixture of the two isolates, and noninoculated control. The test plants were maintained in a screen house, and data on symptom severity and rate of disease development were recorded. ELISA test was conducted to confirm virus presence and distribution. The results indicate that the CABMV isolates used are systemic. There were various differences in disease progression, which was faster in the sweet passion variety than in the purple variety. However, the latter had a higher disease severity. Coinfected plants expressed severe symptoms compared to the singly infected ones, indicating synergistic interaction between the viral isolates. This implies that coinfection has adverse impact resulting in higher economic losses.http://dx.doi.org/10.1155/2020/8876498 |
| spellingShingle | Lucy J. Kiptui Faith J. Toroitich Dora C. Kilalo Meshack Obonyo Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss Advances in Agriculture |
| title | Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss |
| title_full | Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss |
| title_fullStr | Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss |
| title_full_unstemmed | Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss |
| title_short | Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss |
| title_sort | interaction between cowpea aphid borne mosaic virus isolates and its effect on passion fruit woodiness disease on passiflora edulis sims and passiflora ligularis juss |
| url | http://dx.doi.org/10.1155/2020/8876498 |
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