Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis

Abstract Over 60% of women with endometriosis experience abdominopelvic pain and broader pain manifestations, including chronic back pain, fibromyalgia, chronic fatigue, vulvodynia, and migraine. Although the imbalance of proinflammatory mediators, including the complement component C5a, is associat...

Full description

Saved in:
Bibliographic Details
Main Authors: Mustafa Titiz, Lorenzo Landini, Daniel Souza Monteiro de Araujo, Matilde Marini, Viola Seravalli, Martina Chieca, Pasquale Pensieri, Marco Montini, Gaetano De Siena, Benedetta Pasquini, Silvia Vannuccini, Luigi Francesco Iannone, Thiago M. Cunha, Giulia Brancolini, Elisa Bellantoni, Irene Scuffi, Alessandra Mastricci, Martina Tesi, Mariarosaria Di Tommaso, Felice Petraglia, Pierangelo Geppetti, Romina Nassini, Francesco De Logu
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-54486-6
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850064446190256128
author Mustafa Titiz
Lorenzo Landini
Daniel Souza Monteiro de Araujo
Matilde Marini
Viola Seravalli
Martina Chieca
Pasquale Pensieri
Marco Montini
Gaetano De Siena
Benedetta Pasquini
Silvia Vannuccini
Luigi Francesco Iannone
Thiago M. Cunha
Giulia Brancolini
Elisa Bellantoni
Irene Scuffi
Alessandra Mastricci
Martina Tesi
Mariarosaria Di Tommaso
Felice Petraglia
Pierangelo Geppetti
Romina Nassini
Francesco De Logu
author_facet Mustafa Titiz
Lorenzo Landini
Daniel Souza Monteiro de Araujo
Matilde Marini
Viola Seravalli
Martina Chieca
Pasquale Pensieri
Marco Montini
Gaetano De Siena
Benedetta Pasquini
Silvia Vannuccini
Luigi Francesco Iannone
Thiago M. Cunha
Giulia Brancolini
Elisa Bellantoni
Irene Scuffi
Alessandra Mastricci
Martina Tesi
Mariarosaria Di Tommaso
Felice Petraglia
Pierangelo Geppetti
Romina Nassini
Francesco De Logu
author_sort Mustafa Titiz
collection DOAJ
description Abstract Over 60% of women with endometriosis experience abdominopelvic pain and broader pain manifestations, including chronic back pain, fibromyalgia, chronic fatigue, vulvodynia, and migraine. Although the imbalance of proinflammatory mediators, including the complement component C5a, is associated with endometriosis-related pain, the mechanisms causing widespread pain and the C5a role remain unclear. Female mice and women with endometriosis exhibit increased plasma C5a levels and pain. We hypothesize the Schwann cells involvement in endometriotic pain. Here, we show that silencing the C5a receptor (C5aR1) in Schwann cells blocks the C5a-induced activation of the NLRP1 inflammasome and subsequent release of interleukin-1β (IL-1β). Macrophages, recruited to sciatic/trigeminal nerves by IL-1β from Schwann cells, increase oxidative stress, which activates the proalgesic TRPA1 pathway, resulting in widespread pain. These findings reveal a pathway involving Schwann cell C5aR1, NLRP1/IL-1β activation, macrophage recruitment, oxidative stress, and TRPA1 engagement, contributing to pain in a mouse model of endometriosis.
format Article
id doaj-art-9ff86e38bb9f4084816ee6adaec03d94
institution DOAJ
issn 2041-1723
language English
publishDate 2024-11-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-9ff86e38bb9f4084816ee6adaec03d942025-08-20T02:49:17ZengNature PortfolioNature Communications2041-17232024-11-0115111510.1038/s41467-024-54486-6Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosisMustafa Titiz0Lorenzo Landini1Daniel Souza Monteiro de Araujo2Matilde Marini3Viola Seravalli4Martina Chieca5Pasquale Pensieri6Marco Montini7Gaetano De Siena8Benedetta Pasquini9Silvia Vannuccini10Luigi Francesco Iannone11Thiago M. Cunha12Giulia Brancolini13Elisa Bellantoni14Irene Scuffi15Alessandra Mastricci16Martina Tesi17Mariarosaria Di Tommaso18Felice Petraglia19Pierangelo Geppetti20Romina Nassini21Francesco De Logu22Department of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Obstetrics and Gynecology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, Medical Genetics Unit, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Chemistry “U.Schiff”, University of FlorenceDepartment of Experimental and Clinical Biomedical Sciences, Obstetrics and Gynecology Unit, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceCenter for Research in Inflammatory Diseases (CRID), Department of Pharmacology, Ribeirão Preto Medical School, University of São PauloFloNext srlDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Obstetrics and Gynecology Section, University of FlorenceDepartment of Experimental and Clinical Biomedical Sciences, Obstetrics and Gynecology Unit, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceDepartment of Health Science, Clinical Pharmacology and Oncology Section, University of FlorenceAbstract Over 60% of women with endometriosis experience abdominopelvic pain and broader pain manifestations, including chronic back pain, fibromyalgia, chronic fatigue, vulvodynia, and migraine. Although the imbalance of proinflammatory mediators, including the complement component C5a, is associated with endometriosis-related pain, the mechanisms causing widespread pain and the C5a role remain unclear. Female mice and women with endometriosis exhibit increased plasma C5a levels and pain. We hypothesize the Schwann cells involvement in endometriotic pain. Here, we show that silencing the C5a receptor (C5aR1) in Schwann cells blocks the C5a-induced activation of the NLRP1 inflammasome and subsequent release of interleukin-1β (IL-1β). Macrophages, recruited to sciatic/trigeminal nerves by IL-1β from Schwann cells, increase oxidative stress, which activates the proalgesic TRPA1 pathway, resulting in widespread pain. These findings reveal a pathway involving Schwann cell C5aR1, NLRP1/IL-1β activation, macrophage recruitment, oxidative stress, and TRPA1 engagement, contributing to pain in a mouse model of endometriosis.https://doi.org/10.1038/s41467-024-54486-6
spellingShingle Mustafa Titiz
Lorenzo Landini
Daniel Souza Monteiro de Araujo
Matilde Marini
Viola Seravalli
Martina Chieca
Pasquale Pensieri
Marco Montini
Gaetano De Siena
Benedetta Pasquini
Silvia Vannuccini
Luigi Francesco Iannone
Thiago M. Cunha
Giulia Brancolini
Elisa Bellantoni
Irene Scuffi
Alessandra Mastricci
Martina Tesi
Mariarosaria Di Tommaso
Felice Petraglia
Pierangelo Geppetti
Romina Nassini
Francesco De Logu
Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
Nature Communications
title Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
title_full Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
title_fullStr Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
title_full_unstemmed Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
title_short Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
title_sort schwann cell c5ar1 co opts inflammasome nlrp1 to sustain pain in a mouse model of endometriosis
url https://doi.org/10.1038/s41467-024-54486-6
work_keys_str_mv AT mustafatitiz schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT lorenzolandini schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT danielsouzamonteirodearaujo schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT matildemarini schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT violaseravalli schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT martinachieca schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT pasqualepensieri schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT marcomontini schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT gaetanodesiena schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT benedettapasquini schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT silviavannuccini schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT luigifrancescoiannone schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT thiagomcunha schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT giuliabrancolini schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT elisabellantoni schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT irenescuffi schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT alessandramastricci schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT martinatesi schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT mariarosariaditommaso schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT felicepetraglia schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT pierangelogeppetti schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT rominanassini schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis
AT francescodelogu schwanncellc5ar1cooptsinflammasomenlrp1tosustainpaininamousemodelofendometriosis