The intestinal interferon system and specialized enterocytes as putative drivers of HIV latency

The barrier to HIV cure is the HIV reservoir, which is composed of latently infected CD4+ T cells and myeloid cells that carry stably integrated and replication-competent provirus. The gastrointestinal tract (GIT) contains a substantial part of the HIV reservoir and its immunophysiology could be esp...

Full description

Saved in:
Bibliographic Details
Main Authors: Rachel L. Creighton, Sean M. Hughes, Florian Hladik, Germán G. Gornalusse
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Immunology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1589752/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850278677276786688
author Rachel L. Creighton
Rachel L. Creighton
Sean M. Hughes
Sean M. Hughes
Florian Hladik
Florian Hladik
Florian Hladik
Germán G. Gornalusse
Germán G. Gornalusse
Germán G. Gornalusse
author_facet Rachel L. Creighton
Rachel L. Creighton
Sean M. Hughes
Sean M. Hughes
Florian Hladik
Florian Hladik
Florian Hladik
Germán G. Gornalusse
Germán G. Gornalusse
Germán G. Gornalusse
author_sort Rachel L. Creighton
collection DOAJ
description The barrier to HIV cure is the HIV reservoir, which is composed of latently infected CD4+ T cells and myeloid cells that carry stably integrated and replication-competent provirus. The gastrointestinal tract (GIT) contains a substantial part of the HIV reservoir and its immunophysiology could be especially conducive for HIV persistence and reactivation. However, the exact cellular microenvironment and molecular mechanisms that govern the renewal of provirus-harboring cells and proviral reactivation in the GIT remain unclear. In this review, we outline the evidence supporting an overarching hypothesis that interferon activity driven by specialized enterocytes creates a microenvironment that fosters proliferation of latently infected CD4+ T cells and sporadic HIV reactivation from these cells. First, we describe unique immunologic features of the gastrointestinal associated lymphoid tissue (GALT), specifically highlighting IFN activity in specialized enterocytes and potential interactions between these cells and neighboring HIV susceptible cells. Then, we will describe dysregulation of IFN signaling in HIV infection and how IFN dysregulation in the GALT may contribute to the persistence and reactivation of the latent HIV reservoir. Finally, we will speculate on the clinical implications of this hypothesis for HIV cure strategies and outline the next steps.
format Article
id doaj-art-9ff6a7168fbb4afb99784fdad55b2437
institution OA Journals
issn 1664-3224
language English
publishDate 2025-05-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Immunology
spelling doaj-art-9ff6a7168fbb4afb99784fdad55b24372025-08-20T01:49:23ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-05-011610.3389/fimmu.2025.15897521589752The intestinal interferon system and specialized enterocytes as putative drivers of HIV latencyRachel L. Creighton0Rachel L. Creighton1Sean M. Hughes2Sean M. Hughes3Florian Hladik4Florian Hladik5Florian Hladik6Germán G. Gornalusse7Germán G. Gornalusse8Germán G. Gornalusse9Department of Obstetrics and Gynecology, School of Medicine, University of Washington, Seattle, WA, United StatesVaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, United StatesDepartment of Obstetrics and Gynecology, School of Medicine, University of Washington, Seattle, WA, United StatesVaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, United StatesDepartment of Obstetrics and Gynecology, School of Medicine, University of Washington, Seattle, WA, United StatesVaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, United StatesDepartment of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, United StatesDepartment of Obstetrics and Gynecology, School of Medicine, University of Washington, Seattle, WA, United StatesVaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, United StatesDepartment of Global Health, Schools of Medicine and Public Health, University of Washington, Seattle, WA, United StatesThe barrier to HIV cure is the HIV reservoir, which is composed of latently infected CD4+ T cells and myeloid cells that carry stably integrated and replication-competent provirus. The gastrointestinal tract (GIT) contains a substantial part of the HIV reservoir and its immunophysiology could be especially conducive for HIV persistence and reactivation. However, the exact cellular microenvironment and molecular mechanisms that govern the renewal of provirus-harboring cells and proviral reactivation in the GIT remain unclear. In this review, we outline the evidence supporting an overarching hypothesis that interferon activity driven by specialized enterocytes creates a microenvironment that fosters proliferation of latently infected CD4+ T cells and sporadic HIV reactivation from these cells. First, we describe unique immunologic features of the gastrointestinal associated lymphoid tissue (GALT), specifically highlighting IFN activity in specialized enterocytes and potential interactions between these cells and neighboring HIV susceptible cells. Then, we will describe dysregulation of IFN signaling in HIV infection and how IFN dysregulation in the GALT may contribute to the persistence and reactivation of the latent HIV reservoir. Finally, we will speculate on the clinical implications of this hypothesis for HIV cure strategies and outline the next steps.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1589752/fullHIV latencyinterferonenterocytesmicrofold cell (M-cell)interferon stimulated gene (ISG)
spellingShingle Rachel L. Creighton
Rachel L. Creighton
Sean M. Hughes
Sean M. Hughes
Florian Hladik
Florian Hladik
Florian Hladik
Germán G. Gornalusse
Germán G. Gornalusse
Germán G. Gornalusse
The intestinal interferon system and specialized enterocytes as putative drivers of HIV latency
Frontiers in Immunology
HIV latency
interferon
enterocytes
microfold cell (M-cell)
interferon stimulated gene (ISG)
title The intestinal interferon system and specialized enterocytes as putative drivers of HIV latency
title_full The intestinal interferon system and specialized enterocytes as putative drivers of HIV latency
title_fullStr The intestinal interferon system and specialized enterocytes as putative drivers of HIV latency
title_full_unstemmed The intestinal interferon system and specialized enterocytes as putative drivers of HIV latency
title_short The intestinal interferon system and specialized enterocytes as putative drivers of HIV latency
title_sort intestinal interferon system and specialized enterocytes as putative drivers of hiv latency
topic HIV latency
interferon
enterocytes
microfold cell (M-cell)
interferon stimulated gene (ISG)
url https://www.frontiersin.org/articles/10.3389/fimmu.2025.1589752/full
work_keys_str_mv AT rachellcreighton theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT rachellcreighton theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT seanmhughes theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT seanmhughes theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT florianhladik theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT florianhladik theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT florianhladik theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT germanggornalusse theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT germanggornalusse theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT germanggornalusse theintestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT rachellcreighton intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT rachellcreighton intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT seanmhughes intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT seanmhughes intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT florianhladik intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT florianhladik intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT florianhladik intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT germanggornalusse intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT germanggornalusse intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency
AT germanggornalusse intestinalinterferonsystemandspecializedenterocytesasputativedriversofhivlatency