Investigation of Anticancer Peptides Derived from <i>Arca</i> Species Using In Silico Analysis

This study employed an integrated in silico approach to identify and characterize anticancer peptides (ACPs) derived from <i>Arca</i> species. Using a comprehensive bioinformatics pipeline (BIOPEP, ToxinPred, ProtParam, ChemDraw, SwissTargetPrediction, and I-TASSER), we screened hydrolyz...

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Bibliographic Details
Main Authors: Jixu Wu, Xiuhua Zhang, Yuting Jin, Man Zhang, Rongmin Yu, Liyan Song, Fei Liu, Jianhua Zhu
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/7/1640
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Summary:This study employed an integrated in silico approach to identify and characterize anticancer peptides (ACPs) derived from <i>Arca</i> species. Using a comprehensive bioinformatics pipeline (BIOPEP, ToxinPred, ProtParam, ChemDraw, SwissTargetPrediction, and I-TASSER), we screened hydrolyzed bioactive peptides from <i>Arca</i> species, identifying seventeen novel peptide candidates. Subsequent in vitro validation revealed three peptides (KW, WQIWYK, KGKWQIWYKSL) with significant anticancer activity, demonstrating both high biosafety and clinical potential. Our findings highlight <i>Arca</i> species proteins as a valuable source of therapeutic ACPs and establish bioinformatics as an efficient strategy for rapid discovery of bioactive peptides. This approach combines computational prediction with experimental validation, offering a robust framework for developing novel peptide-based therapeutics.
ISSN:1420-3049