Insights into the Genome Sequence of Chromobacterium amazonense Isolated from a Tropical Freshwater Lake
Members of the genus Chromobacterium have been isolated from geographically diverse ecosystems and exhibit considerable metabolic flexibility, as well as biotechnological and pathogenic properties in some species. This study reports the draft assembly and detailed sequence analysis of Chromobacteriu...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/1062716 |
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| author | Alexandre Bueno Santos Patrícia Silva Costa Anderson Oliveira do Carmo Gabriel da Rocha Fernandes Larissa Lopes Silva Scholte Jeronimo Ruiz Evanguedes Kalapothakis Edmar Chartone-Souza Andréa Maria Amaral Nascimento |
| author_facet | Alexandre Bueno Santos Patrícia Silva Costa Anderson Oliveira do Carmo Gabriel da Rocha Fernandes Larissa Lopes Silva Scholte Jeronimo Ruiz Evanguedes Kalapothakis Edmar Chartone-Souza Andréa Maria Amaral Nascimento |
| author_sort | Alexandre Bueno Santos |
| collection | DOAJ |
| description | Members of the genus Chromobacterium have been isolated from geographically diverse ecosystems and exhibit considerable metabolic flexibility, as well as biotechnological and pathogenic properties in some species. This study reports the draft assembly and detailed sequence analysis of Chromobacterium amazonense strain 56AF. The de novo-assembled genome is 4,556,707 bp in size and contains 4294 protein-coding and 95 RNA genes, including 88 tRNA, six rRNA, and one tmRNA operon. A repertoire of genes implicated in virulence, for example, hemolysin, hemolytic enterotoxins, colicin V, lytic proteins, and Nudix hydrolases, is present. The genome also contains a collection of genes of biotechnological interest, including esterases, lipase, auxins, chitinases, phytoene synthase and phytoene desaturase, polyhydroxyalkanoates, violacein, plastocyanin/azurin, and detoxifying compounds. Importantly, unlike other Chromobacterium species, the 56AF genome contains genes for pore-forming toxin alpha-hemolysin, a type IV secretion system, among others. The analysis of the C. amazonense strain 56AF genome reveals the versatility, adaptability, and biotechnological potential of this bacterium. This study provides molecular information that may pave the way for further comparative genomics and functional studies involving Chromobacterium-related isolates and improves our understanding of the global genomic diversity of Chromobacterium species. |
| format | Article |
| id | doaj-art-9ff38173486c46af9ca58b92eed3c8aa |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-9ff38173486c46af9ca58b92eed3c8aa2025-02-03T05:53:05ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/10627161062716Insights into the Genome Sequence of Chromobacterium amazonense Isolated from a Tropical Freshwater LakeAlexandre Bueno Santos0Patrícia Silva Costa1Anderson Oliveira do Carmo2Gabriel da Rocha Fernandes3Larissa Lopes Silva Scholte4Jeronimo Ruiz5Evanguedes Kalapothakis6Edmar Chartone-Souza7Andréa Maria Amaral Nascimento8Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilDepartamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilDepartamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilCentro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, BrazilCentro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, BrazilCentro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, BrazilDepartamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilDepartamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilDepartamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilMembers of the genus Chromobacterium have been isolated from geographically diverse ecosystems and exhibit considerable metabolic flexibility, as well as biotechnological and pathogenic properties in some species. This study reports the draft assembly and detailed sequence analysis of Chromobacterium amazonense strain 56AF. The de novo-assembled genome is 4,556,707 bp in size and contains 4294 protein-coding and 95 RNA genes, including 88 tRNA, six rRNA, and one tmRNA operon. A repertoire of genes implicated in virulence, for example, hemolysin, hemolytic enterotoxins, colicin V, lytic proteins, and Nudix hydrolases, is present. The genome also contains a collection of genes of biotechnological interest, including esterases, lipase, auxins, chitinases, phytoene synthase and phytoene desaturase, polyhydroxyalkanoates, violacein, plastocyanin/azurin, and detoxifying compounds. Importantly, unlike other Chromobacterium species, the 56AF genome contains genes for pore-forming toxin alpha-hemolysin, a type IV secretion system, among others. The analysis of the C. amazonense strain 56AF genome reveals the versatility, adaptability, and biotechnological potential of this bacterium. This study provides molecular information that may pave the way for further comparative genomics and functional studies involving Chromobacterium-related isolates and improves our understanding of the global genomic diversity of Chromobacterium species.http://dx.doi.org/10.1155/2018/1062716 |
| spellingShingle | Alexandre Bueno Santos Patrícia Silva Costa Anderson Oliveira do Carmo Gabriel da Rocha Fernandes Larissa Lopes Silva Scholte Jeronimo Ruiz Evanguedes Kalapothakis Edmar Chartone-Souza Andréa Maria Amaral Nascimento Insights into the Genome Sequence of Chromobacterium amazonense Isolated from a Tropical Freshwater Lake International Journal of Genomics |
| title | Insights into the Genome Sequence of Chromobacterium amazonense Isolated from a Tropical Freshwater Lake |
| title_full | Insights into the Genome Sequence of Chromobacterium amazonense Isolated from a Tropical Freshwater Lake |
| title_fullStr | Insights into the Genome Sequence of Chromobacterium amazonense Isolated from a Tropical Freshwater Lake |
| title_full_unstemmed | Insights into the Genome Sequence of Chromobacterium amazonense Isolated from a Tropical Freshwater Lake |
| title_short | Insights into the Genome Sequence of Chromobacterium amazonense Isolated from a Tropical Freshwater Lake |
| title_sort | insights into the genome sequence of chromobacterium amazonense isolated from a tropical freshwater lake |
| url | http://dx.doi.org/10.1155/2018/1062716 |
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