Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETP
Abstract Background and objectives Several thrombin generation tests are available but few have been directly compared. Our primary aim was to investigate the correlation of two thrombin generation tests, thrombin generation assay-calibrated automated thrombogram (TGA-CAT) and INNOVANCE ETP, to fa...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2017-11-01
|
| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.mjhid.org/index.php/mjhid/article/view/3097 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850058823358742528 |
|---|---|
| author | Marcus Ljungkvist |
| author_facet | Marcus Ljungkvist |
| author_sort | Marcus Ljungkvist |
| collection | DOAJ |
| description | Abstract
Background and objectives
Several thrombin generation tests are available but few have been directly compared. Our primary aim was to investigate the correlation of two thrombin generation tests, thrombin generation assay-calibrated automated thrombogram (TGA-CAT) and INNOVANCE ETP, to factor VIII levels (FVIII:C) in a group of patients with hemophilia A.
The secondary aim was to investigate inter-laboratory variation for the TGA-CAT method.
Methods
Blood samples were taken from 45 patients with mild, moderate and severe hemophilia A. The TGA-CAT method was performed at both centers while the INNOVANCE ETP was only performed at the Stockholm center. Correlation between parameters was evaluated using Spearman´s rank correlation test. For determination of the TGA-CAT inter-laboratory variability Bland-Altman plots was used.
Results
The correlation for the INNOVANCE ETP and TGA-CAT methods with FVIII:C in persons with hemophilia (PWH) was r=0,701 and r=0,734 respectively.
The correlation between the two methods was r=0,546.
When dividing the study material into disease severity groups (mild, moderate and severe) based on FVIII levels, both methods fail to discriminate between them.
The variability of the TGA-CAT results performed at the two centers was reduced after normalization, before normalization 29% of values showed less than ±10% difference while after normalization the number increased to 41%.
Conclusions
Both methods correlate in an equal manner to FVIII:C in PWH but show a poor correlation to each other. The level of agreement for the TGA-CAT method was poor though slightly improved after normalization of data. Further improvement of standardization of these methods is warranted. |
| format | Article |
| id | doaj-art-9fea59f1cc1442eaa70ca32252b2fea0 |
| institution | DOAJ |
| issn | 2035-3006 |
| language | English |
| publishDate | 2017-11-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj-art-9fea59f1cc1442eaa70ca32252b2fea02025-08-20T02:51:02ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062017-11-0191e2017064e201706410.4084/mjhid.2017.0641719Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETPMarcus Ljungkvist0Department of Translational Medicine & Centre for Thrombosis and Haemostasis, Malmö, Lund University, SwedenAbstract Background and objectives Several thrombin generation tests are available but few have been directly compared. Our primary aim was to investigate the correlation of two thrombin generation tests, thrombin generation assay-calibrated automated thrombogram (TGA-CAT) and INNOVANCE ETP, to factor VIII levels (FVIII:C) in a group of patients with hemophilia A. The secondary aim was to investigate inter-laboratory variation for the TGA-CAT method. Methods Blood samples were taken from 45 patients with mild, moderate and severe hemophilia A. The TGA-CAT method was performed at both centers while the INNOVANCE ETP was only performed at the Stockholm center. Correlation between parameters was evaluated using Spearman´s rank correlation test. For determination of the TGA-CAT inter-laboratory variability Bland-Altman plots was used. Results The correlation for the INNOVANCE ETP and TGA-CAT methods with FVIII:C in persons with hemophilia (PWH) was r=0,701 and r=0,734 respectively. The correlation between the two methods was r=0,546. When dividing the study material into disease severity groups (mild, moderate and severe) based on FVIII levels, both methods fail to discriminate between them. The variability of the TGA-CAT results performed at the two centers was reduced after normalization, before normalization 29% of values showed less than ±10% difference while after normalization the number increased to 41%. Conclusions Both methods correlate in an equal manner to FVIII:C in PWH but show a poor correlation to each other. The level of agreement for the TGA-CAT method was poor though slightly improved after normalization of data. Further improvement of standardization of these methods is warranted.http://www.mjhid.org/index.php/mjhid/article/view/3097thrombin generation test (TGT), thrombin generation assay-calibrated automated thrombogram (TGA-CAT), INNOVANCE ETP, platelet-poor plasma (PPP), platelet-rich plasma (PRP), test of agreement, FVIII:C, hemophilia |
| spellingShingle | Marcus Ljungkvist Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETP Mediterranean Journal of Hematology and Infectious Diseases thrombin generation test (TGT), thrombin generation assay-calibrated automated thrombogram (TGA-CAT), INNOVANCE ETP, platelet-poor plasma (PPP), platelet-rich plasma (PRP), test of agreement, FVIII:C, hemophilia |
| title | Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETP |
| title_full | Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETP |
| title_fullStr | Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETP |
| title_full_unstemmed | Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETP |
| title_short | Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETP |
| title_sort | correlation to fviii c in two thrombin generation tests tga cat and innovance etp |
| topic | thrombin generation test (TGT), thrombin generation assay-calibrated automated thrombogram (TGA-CAT), INNOVANCE ETP, platelet-poor plasma (PPP), platelet-rich plasma (PRP), test of agreement, FVIII:C, hemophilia |
| url | http://www.mjhid.org/index.php/mjhid/article/view/3097 |
| work_keys_str_mv | AT marcusljungkvist correlationtofviiicintwothrombingenerationteststgacatandinnovanceetp |