Investigating the Activities of <i>CAF20</i> and <i>ECM32</i> in the Regulation of <i>PGM2</i> mRNA Translation
Translation is a fundamental process in biology, and understanding its mechanisms is crucial to comprehending cellular functions and diseases. The regulation of this process is closely linked to the structure of mRNA, as these regions prove vital to modulating translation efficiency and control. Thu...
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2024-10-01
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| author | Mustafa Al-gafari Sasi Kumar Jagadeesan Thomas David Daniel Kazmirchuk Sarah Takallou Jiashu Wang Maryam Hajikarimlou Nishka Beersing Ramessur Waleed Darwish Calvin Bradbury-Jost Houman Moteshareie Kamaledin B. Said Bahram Samanfar Ashkan Golshani |
| author_facet | Mustafa Al-gafari Sasi Kumar Jagadeesan Thomas David Daniel Kazmirchuk Sarah Takallou Jiashu Wang Maryam Hajikarimlou Nishka Beersing Ramessur Waleed Darwish Calvin Bradbury-Jost Houman Moteshareie Kamaledin B. Said Bahram Samanfar Ashkan Golshani |
| author_sort | Mustafa Al-gafari |
| collection | DOAJ |
| description | Translation is a fundamental process in biology, and understanding its mechanisms is crucial to comprehending cellular functions and diseases. The regulation of this process is closely linked to the structure of mRNA, as these regions prove vital to modulating translation efficiency and control. Thus, identifying and investigating these fundamental factors that influence the processing and unwinding of structured mRNAs would be of interest due to the widespread impact in various fields of biology. To this end, we employed a computational approach and identified genes that may be involved in the translation of structured mRNAs. The approach is based on the enrichment of interactions and co-expression of genes with those that are known to influence translation and helicase activity. The in silico prediction found <i>CAF20</i> and <i>ECM32</i> to be highly ranked candidates that may play a role in unwinding mRNA. The activities of neither <i>CAF20</i> nor <i>ECM32</i> have previously been linked to the translation of <i>PGM2</i> mRNA or other structured mRNAs. Our follow-up investigations with these two genes provided evidence of their participation in the translation of <i>PGM2</i> mRNA and several other synthetic structured mRNAs. |
| format | Article |
| id | doaj-art-9fdf2e76641d4848a67883058eaf0dfa |
| institution | OA Journals |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-9fdf2e76641d4848a67883058eaf0dfa2025-08-20T01:53:40ZengMDPI AGBiology2079-77372024-10-01131188410.3390/biology13110884Investigating the Activities of <i>CAF20</i> and <i>ECM32</i> in the Regulation of <i>PGM2</i> mRNA TranslationMustafa Al-gafari0Sasi Kumar Jagadeesan1Thomas David Daniel Kazmirchuk2Sarah Takallou3Jiashu Wang4Maryam Hajikarimlou5Nishka Beersing Ramessur6Waleed Darwish7Calvin Bradbury-Jost8Houman Moteshareie9Kamaledin B. Said10Bahram Samanfar11Ashkan Golshani12Department of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaOttawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1N 6N5, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaTranslation is a fundamental process in biology, and understanding its mechanisms is crucial to comprehending cellular functions and diseases. The regulation of this process is closely linked to the structure of mRNA, as these regions prove vital to modulating translation efficiency and control. Thus, identifying and investigating these fundamental factors that influence the processing and unwinding of structured mRNAs would be of interest due to the widespread impact in various fields of biology. To this end, we employed a computational approach and identified genes that may be involved in the translation of structured mRNAs. The approach is based on the enrichment of interactions and co-expression of genes with those that are known to influence translation and helicase activity. The in silico prediction found <i>CAF20</i> and <i>ECM32</i> to be highly ranked candidates that may play a role in unwinding mRNA. The activities of neither <i>CAF20</i> nor <i>ECM32</i> have previously been linked to the translation of <i>PGM2</i> mRNA or other structured mRNAs. Our follow-up investigations with these two genes provided evidence of their participation in the translation of <i>PGM2</i> mRNA and several other synthetic structured mRNAs.https://www.mdpi.com/2079-7737/13/11/884functional genomicslithium chloridemRNA helicaseyeasttranslation regulationstructured mRNA |
| spellingShingle | Mustafa Al-gafari Sasi Kumar Jagadeesan Thomas David Daniel Kazmirchuk Sarah Takallou Jiashu Wang Maryam Hajikarimlou Nishka Beersing Ramessur Waleed Darwish Calvin Bradbury-Jost Houman Moteshareie Kamaledin B. Said Bahram Samanfar Ashkan Golshani Investigating the Activities of <i>CAF20</i> and <i>ECM32</i> in the Regulation of <i>PGM2</i> mRNA Translation Biology functional genomics lithium chloride mRNA helicase yeast translation regulation structured mRNA |
| title | Investigating the Activities of <i>CAF20</i> and <i>ECM32</i> in the Regulation of <i>PGM2</i> mRNA Translation |
| title_full | Investigating the Activities of <i>CAF20</i> and <i>ECM32</i> in the Regulation of <i>PGM2</i> mRNA Translation |
| title_fullStr | Investigating the Activities of <i>CAF20</i> and <i>ECM32</i> in the Regulation of <i>PGM2</i> mRNA Translation |
| title_full_unstemmed | Investigating the Activities of <i>CAF20</i> and <i>ECM32</i> in the Regulation of <i>PGM2</i> mRNA Translation |
| title_short | Investigating the Activities of <i>CAF20</i> and <i>ECM32</i> in the Regulation of <i>PGM2</i> mRNA Translation |
| title_sort | investigating the activities of i caf20 i and i ecm32 i in the regulation of i pgm2 i mrna translation |
| topic | functional genomics lithium chloride mRNA helicase yeast translation regulation structured mRNA |
| url | https://www.mdpi.com/2079-7737/13/11/884 |
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