Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approach
This study systematically explored the role of NEDD8 in pediatric acute myeloid leukemia (AML) through patient sample analysis, database mining, and in vitro experiments. Our results demonstrated that NEDD8 was significantly overexpressed in newly diagnosed pediatric AML patients and was associated...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Hematology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2025.2478650 |
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| author | Jian Sun Cui Liu Guangli Yang Qian Li Yang An Yin Zhu Pingping Zhang Yaning Guan Chang Peng Zuochen Du Pei Huang Yan Chen |
| author_facet | Jian Sun Cui Liu Guangli Yang Qian Li Yang An Yin Zhu Pingping Zhang Yaning Guan Chang Peng Zuochen Du Pei Huang Yan Chen |
| author_sort | Jian Sun |
| collection | DOAJ |
| description | This study systematically explored the role of NEDD8 in pediatric acute myeloid leukemia (AML) through patient sample analysis, database mining, and in vitro experiments. Our results demonstrated that NEDD8 was significantly overexpressed in newly diagnosed pediatric AML patients and was associated with poor survival outcomes. Functional enrichment analysis of the TARGET database further revealed a strong correlation between NEDD8 and cancer-related pathways. In vitro experiments showed that NEDD8 knockdown significantly inhibited the proliferation of AML cells (THP-1 and MV4-11) and induced cell cycle arrest. Collectively, these findings highlight the critical role of NEDD8 in pediatric AML pathogenesis and suggest its potential as both a prognostic biomarker and a therapeutic target. |
| format | Article |
| id | doaj-art-9fd8dd6fb766470ca91592afb1efa73c |
| institution | OA Journals |
| issn | 1607-8454 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hematology |
| spelling | doaj-art-9fd8dd6fb766470ca91592afb1efa73c2025-08-20T02:07:16ZengTaylor & Francis GroupHematology1607-84542025-12-0130110.1080/16078454.2025.2478650Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approachJian Sun0Cui Liu1Guangli Yang2Qian Li3Yang An4Yin Zhu5Pingping Zhang6Yaning Guan7Chang Peng8Zuochen Du9Pei Huang10Yan Chen11Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaDepartment of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, People’s Republic of ChinaThis study systematically explored the role of NEDD8 in pediatric acute myeloid leukemia (AML) through patient sample analysis, database mining, and in vitro experiments. Our results demonstrated that NEDD8 was significantly overexpressed in newly diagnosed pediatric AML patients and was associated with poor survival outcomes. Functional enrichment analysis of the TARGET database further revealed a strong correlation between NEDD8 and cancer-related pathways. In vitro experiments showed that NEDD8 knockdown significantly inhibited the proliferation of AML cells (THP-1 and MV4-11) and induced cell cycle arrest. Collectively, these findings highlight the critical role of NEDD8 in pediatric AML pathogenesis and suggest its potential as both a prognostic biomarker and a therapeutic target.https://www.tandfonline.com/doi/10.1080/16078454.2025.2478650Acute myeloid leukemiapediatricNEDD8prognosiscell cycle |
| spellingShingle | Jian Sun Cui Liu Guangli Yang Qian Li Yang An Yin Zhu Pingping Zhang Yaning Guan Chang Peng Zuochen Du Pei Huang Yan Chen Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approach Hematology Acute myeloid leukemia pediatric NEDD8 prognosis cell cycle |
| title | Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approach |
| title_full | Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approach |
| title_fullStr | Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approach |
| title_full_unstemmed | Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approach |
| title_short | Targeting NEDD8 in pediatric acute myeloid leukemia: an integrated bioinformatics and experimental approach |
| title_sort | targeting nedd8 in pediatric acute myeloid leukemia an integrated bioinformatics and experimental approach |
| topic | Acute myeloid leukemia pediatric NEDD8 prognosis cell cycle |
| url | https://www.tandfonline.com/doi/10.1080/16078454.2025.2478650 |
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